Restriction map for
pFN21AE5131
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I TseI Tsp509I Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 76 GTAC GT^AC
AlwI 99 GGATC GGATC(4/5)
AlwNI 67 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 399 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 247 CTCGAG C^[CT]CG[GA]G
AviII 233 TGCGCA TGC^GCA
BcnI 6 CCGGG CC^[GC]GG
BsiYI 216 CCAAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 216 CCAAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 247 CTCGAG C^[CT]CG[GA]G
BspMI 66 GCAGGT ACCTGC(4/8)
BsrFI 126 GCCGGC [GA]^CCGG[CT]
BstYI 99 GGATCT [GA]^GATC[CT]
Cfr10I 126 GCCGGC [GA]^CCGG[CT]
DpnII 100 GATC ^GATC
DsaI 167 CCGCGG C^C[GA][CT]GG
EcoT14I 374 CCAAGG C^C[AT][AT]GG
FspI 233 TGCGCA TGC^GCA
HphI 183 TCACC GGTGA(8/7)
KspI 167 CCGCGG CCGC^GG
MboI 100 GATC ^GATC
MflI 99 GGATCT [GA]^GATC[CT]
MspA1I 167 CCGCGG C[AC]G^C[GT]G
NaeI 126 GCCGGC GCC^GGC
NciI 6 CCGGG CC^[GC]GG
NdeII 100 GATC ^GATC
NgoMIV 126 GCCGGC G^CCGGC
PaeR7I 247 CTCGAG C^TCGAG
PflMI 216 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 68 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 76 GTAC GT^AC
SacII 167 CCGCGG CCGC^GG
Sau3AI 100 GATC ^GATC
SfiI 162 GGCCACCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 230 GCATGC GCATG^C
StyI 374 CCAAGG C^C[AT][AT]GG
TaqI 248 TCGA T^CGA
Tsp45I 155 GTCAC ^GT[GC]AC
TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 248 TCGA T^CGA
Van91I 216 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 374 CCAAGGAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 247 CTCGAG C^TCGAG
XhoII 99 GGATCT [GA]^GATC[CT]
XmnI 399 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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BglI 49 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
163 GCCACCGCGGC
BsaI 262 GAGACC GGTCTC(1/5)
286 GAGACC
BsmAI 262 GAGAC GTCTC(1/5)
286 GAGAC
BsrI 138 ACTGG ACTGG(1/-1)
390 CCAGT
BstNI 72 CCTGG CC^[AT]GG
122 CCTGG
DraII 68 AGGTCCT [GA]G^G[ACGT]CC[CT]
251 AGGCCCC
EcoO109I 68 AGGTCCT [GA]G^G[ACGT]CC[CT]
251 AGGCCCC
EcoRII 72 CCTGG ^CC[AT]GG
122 CCTGG
FauI 195 CCCGC CCCGC(4/6)
130 GCGGG
MaeIII 90 GTTAC ^GT[ACGT]AC
155 GTCAC
MvaI 72 CCTGG CC^[AT]GG
122 CCTGG
NlaIII 231 CATG CATG^
315 CATG
NlaIV 60 GGAGCC GG[ACGT]^[ACGT]CC
252 GGCCCC
NspI 230 GCATGC [GA]CATG^[CT]
314 ACATGC
SmlI 247 CTCGAG C^T[CT][GA]AG
325 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AluI AvaII BsaJI
BssKI BstUI Cac8I CfoI Cfr13I
EaeI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MnlI
MspI MvnI MwoI Sau96I ScrFI