Restriction map for
pFN21AE3528
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 2310 GTCTAC GT^[AC][GT]AC
Alw44I 1661 GTGCAC G^TGCAC
AocI 2725 CCTCAGG CC^T[ACGT]AGG
ApaLI 1661 GTGCAC G^TGCAC
AvaI 670 CTCGGG C^[CT]CG[GA]G
BamHI 2451 GGATCC G^GATCC
BanII 2499 GAGCTC G[GA]GC[CT]^C
BclI 2006 TGATCA T^GATCA
BglII 1419 AGATCT A^GATCT
BlpI 156 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 156 GCTGAGC GC^T[ACGT]AGC
BsmFI 952 GTCCC GGGAC(10/14)
BsoBI 670 CTCGGG C^[CT]CG[GA]G
Bsp1407I 528 TGTACA T^GTACA
BspHI 1119 TCATGA T^CATGA
BspLU11I 465 ACATGT A^CATGT
BsrDI 1622 GCAATG GCAATG(2/0)
BsrGI 528 TGTACA T^GTACA
BstEII 793 GGTGACC G^GT[ACGT]ACC
BstPI 793 GGTGACC G^GT[ACGT]ACC
BstXI 42 CCAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2725 CCTCAGG CC^T[ACGT]AGG
CelII 156 GCTGAGC GC^T[ACGT]AGC
DraI 1429 TTTAAA TTT^AAA
DraII 1514 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 955 CCATGG C^C[GA][CT]GG
Eco81I 2725 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1514 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 793 GGTGACC G^GT[ACGT]ACC
EcoRI 1410 GAATTC G^AATTC
EcoRV 3 GATATC GAT^ATC
EcoT22I 1228 ATGCAT ATGCA^T
FbaI 2006 TGATCA T^GATCA
HindIII 1316 AAGCTT A^AGCTT
MslI 1248 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 955 CCATGG C^CATGG
NsiI 1228 ATGCAT ATGCA^T
PpuMI 1514 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 847 CTGCAG CTGCA^G
RcaI 1119 TCATGA T^CATGA
SacI 2499 GAGCTC GAGCT^C
SapI 1438 GAAGAGC GCTCTTC(1/4)
SspBI 528 TGTACA T^GTACA
SspI 1089 AATATT AAT^ATT
TaqI 2033 TCGA T^CGA
TthHB8I 2033 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AflIII 236 ACGTGT A^C[GA][CT]GT
465 ACATGT
AlwNI 841 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1937 CAGTGGCTG
AspI 203 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2116 GACAGTGTC
BbsI 45 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BpuAI 45 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BseRI 2215 GAGGAG GAGGAG(10/8)
2665 GAGGAG
BsgI 1372 CTGCAC GTGCAG(16/14)
1877 CTGCAC
BsmI 888 GAATGC GAATGC(1/-1)
1797 GAATGC
BspMI 212 ACCTGC ACCTGC(4/8)
1370 ACCTGC
MaeII 236 ACGT A^CGT
2438 ACGT
MwoI 843 GCAACTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1622 GCAATGAAAGC
PflFI 203 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2116 GACAGTGTC
StuI 2462 AGGCCT AGG^CCT
2552 AGGCCT
Tth111I 203 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2116 GACAGTGTC
XcmI 1760 CCAGTGATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1818 CCAGAGAGACTGTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
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AcsI AfaI AluI AlwI ApoI
Asp700I AspHI AvaII BanI BbvI
BfaI BmyI BpmI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI