Restriction map for
pFN21AE2174
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 141 TCCGGA T^CCGGA
AcsI 283 AAATTC [GA]^AATT[CT]
AcyI 532 GACGCC G[GA]^CG[CT]C
AhdI 391 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 564 GATCC GGATC(4/5)
ApaI 337 GGGCCC GGGCC^C
ApoI 283 AAATTC [GA]^AATT[CT]
AspEI 391 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 610 GAGCAC G[AT]GC[AT]^C
AspI 538 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 414 CCTCAGC CCTCAGC(-5/-2)
BsaHI 532 GACGCC G[GA]^CG[CT]C
BsaI 43 GAGACC GGTCTC(1/5)
BseAI 141 TCCGGA T^CCGGA
BsiHKAI 610 GAGCAC G[AT]GC[AT]^C
BsmBI 769 GAGACG CGTCTC(1/5)
BspEI 141 TCCGGA T^CCGGA
BsrFI 551 GCCGGT [GA]^CCGG[CT]
BsrI 443 CCAGT ACTGG(1/-1)
BstXI 341 CCAGGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 551 GCCGGT [GA]^CCGG[CT]
DraIII 616 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 689 TGGCCG [CT]^GGCC[GA]
Eam1105I 391 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 532 GACGCC G[GA]^CG[CT]C
HinfI 397 GAGTC G^A[ACGT]TC
HphI 821 TCACC GGTGA(8/7)
MroI 141 TCCGGA T^CCGGA
NspI 593 GCATGT [GA]CATG^[CT]
PaeR7I 730 CTCGAG C^TCGAG
PflFI 538 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 615 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 397 GAGTC GAGTC(4/5)
PstI 223 CTGCAG CTGCA^G
SapI 798 GAAGAGC GCTCTTC(1/4)
ScaI 734 AGTACT AGT^ACT
SfaNI 233 GCATC GCATC(5/9)
SfcI 223 CTGCAG C^T[GA][CT]AG
SmaI 509 CCCGGG CCC^GGG
StuI 383 AGGCCT AGG^CCT
Tsp509I 284 AATT ^AATT
Tth111I 538 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 615 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 730 CTCGAG C^TCGAG
XmaCI 509 CCCGGG C^CCGGG
XmaI 509 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 599 CGCG CG^CG
619 CGCG
AfaI 555 GTAC GT^AC
735 GTAC
BanII 111 GAGCCC G[GA]GC[CT]^C
337 GGGCCC
BcnI 509 CCCGG CC^[GC]GG
510 CCGGG
BfaI 85 CTAG C^TAG
479 CTAG
BglII 53 AGATCT A^GATCT
95 AGATCT
BpmI 205 CTGGAG CTGGAG(16/14)
244 CTGGAG
BsaWI 8 ACCGGA [AT]^CCGG[AT]
141 TCCGGA
BsgI 193 CTGCAC GTGCAG(16/14)
784 GTGCAG
BsmAI 43 GAGAC GTCTC(1/5)
769 GAGAC
BsmFI 257 GGGAC GGGAC(10/14)
356 GGGAC
BstUI 599 CGCG CG^CG
619 CGCG
DraII 239 AGGACCT [GA]G^G[ACGT]CC[CT]
542 GGGTCCT
DsaI 363 CCGTGG C^C[GA][CT]GG
635 CCGTGG
EcoO109I 239 AGGACCT [GA]G^G[ACGT]CC[CT]
542 GGGTCCT
EcoT14I 503 CCAAGG C^C[AT][AT]GG
716 CCAAGG
FauI 740 CCCGC CCCGC(4/6)
600 GCGGG
MaeI 85 CTAG C^TAG
479 CTAG
MvnI 599 CGCG CG^CG
619 CGCG
NciI 509 CCCGG CC^[GC]GG
510 CCGGG
NlaIII 198 CATG CATG^
594 CATG
PpuMI 239 AGGACCT [GA]G^G[AT]CC[CT]
542 GGGTCCT
PvuII 190 CAGCTG CAG^CTG
274 CAGCTG
RsaI 555 GTAC GT^AC
735 GTAC
StyI 503 CCAAGG C^C[AT][AT]GG
716 CCAAGG
TspRI 319 CACTG CA[GC]TG[ACGT][ACGT]^
444 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AvaI AvaII BanI
BbvI BmyI BsaJI BseRI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI Ksp632I
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SmlI TaqI
TseI TthHB8I XhoII