Restriction map for
pFN21AE3067
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BciVI BclI BfaI BfrI BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MseI
MslI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SnaBI SpeI Sse8387I
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 607 CGCG CG^CG
AflIII 330 ACACGT A^C[GA][CT]GT
AocI 148 CCTGAGG CC^T[ACGT]AGG
Asp700I 201 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 292 GTGCTC G[AT]GC[AT]^C
BbvCI 571 GCTGAGG CCTCAGC(-5/-2)
BbvI 654 GCTGC GCAGC(8/12)
BcgI 423 CGAGGAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 499 GCCGGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 313 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC
BsaAI 331 CACGTA [CT]AC^GT[GA]
BsgI 380 CTGCAC GTGCAG(16/14)
BsiEI 501 CGGTCG CG[GA][CT]^CG
BsiHKAI 292 GTGCTC G[AT]GC[AT]^C
Bsp1407I 47 TGTACA T^GTACA
BsrGI 47 TGTACA T^GTACA
BstEII 132 GGTCACC G^GT[ACGT]ACC
BstPI 132 GGTCACC G^GT[ACGT]ACC
BstUI 607 CGCG CG^CG
Bsu36I 148 CCTGAGG CC^T[ACGT]AGG
CelII 313 GCTGAGC GC^T[ACGT]AGC
DrdI 124 GACCTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 148 CCTGAGG CC^T[ACGT]AGG
EcoO65I 132 GGTCACC G^GT[ACGT]ACC
HgaI 284 GCGTC GACGC(5/10)
HincII 286 GTCAAC GT[CT]^[GA]AC
HindII 286 GTCAAC GT[CT]^[GA]AC
HindIII 527 AAGCTT A^AGCTT
HinfI 376 GAGTC G^A[ACGT]TC
MboII 248 TCTTC GAAGA(8/7)
MspA1I 483 CAGCGG C[AC]G^C[GT]G
MvnI 607 CGCG CG^CG
NaeI 472 GCCGGC GCC^GGC
NcoI 581 CCATGG C^CATGG
NdeI 161 CATATG CA^TATG
NgoMIV 472 GCCGGC G^CCGGC
NspI 594 GCATGC [GA]CATG^[CT]
PleI 376 GAGTC GAGTC(4/5)
SgrAI 471 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 561 CCCGGG CCC^GGG
SmlI 295 CTCAAG C^T[CT][GA]AG
SphI 594 GCATGC GCATG^C
SspBI 47 TGTACA T^GTACA
TaqI 140 TCGA T^CGA
TseI 654 GCTGC G^C[AT]GC
TthHB8I 140 TCGA T^CGA
XmaCI 561 CCCGGG C^CCGGG
XmaI 561 CCCGGG C^CCGGG
XmnI 201 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 265 GGCGCC G[GA]^CG[CT]C
469 GGCGCC
AfaI 48 GTAC GT^AC
334 GTAC
AlwI 360 GATCC GGATC(4/5)
459 GATCC
BalI 512 TGGCCA TGG^CCA
584 TGGCCA
BanI 265 GGCGCC G^G[CT][GA]CC
469 GGCGCC
BbeI 265 GGCGCC GGCGC^C
469 GGCGCC
BcnI 561 CCCGG CC^[GC]GG
562 CCGGG
BglII 245 AGATCT A^GATCT
278 AGATCT
BpmI 127 CTCCAG CTGGAG(16/14)
167 CTGGAG
BsaHI 265 GGCGCC G[GA]^CG[CT]C
469 GGCGCC
BsrFI 472 GCCGGC [GA]^CCGG[CT]
499 GCCGGT
BsrI 305 ACTGG ACTGG(1/-1)
515 CCAGT
BstF5I 36 CATCC GGATG(2/0)
324 CATCC
Cfr10I 472 GCCGGC [GA]^CCGG[CT]
499 GCCGGT
DsaI 462 CCACGG C^C[GA][CT]GG
581 CCATGG
EcoT14I 224 CCAAGG C^C[AT][AT]GG
581 CCATGG
FokI 36 CATCC GGATG(9/13)
324 CATCC
Hin1I 265 GGCGCC G[GA]^CG[CT]C
469 GGCGCC
KasI 265 GGCGCC G^GCGCC
469 GGCGCC
MaeIII 58 GTGAC ^GT[ACGT]AC
133 GTCAC
MluNI 512 TGGCCA TGG^CCA
584 TGGCCA
MscI 512 TGGCCA TGG^CCA
584 TGGCCA
NarI 265 GGCGCC GG^CGCC
469 GGCGCC
NciI 561 CCCGG CC^[GC]GG
562 CCGGG
PflMI 79 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
160 CCATATGCTGG
RsaI 48 GTAC GT^AC
334 GTAC
SfaNI 89 GCATC GCATC(5/9)
323 GCATC
SfoI 265 GGCGCC GGC^GCC
469 GGCGCC
StyI 224 CCAAGG C^C[AT][AT]GG
581 CCATGG
Tsp45I 58 GTGAC ^GT[GC]AC
133 GTCAC
Van91I 79 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
160 CCATATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI ApaI AvaI AvaII
BanII BmyI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MboI MflI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
TspRI XhoII