Restriction map for
pFN21AB7544
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaII AviII AvrII BalI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfaNI SfiI SfuI SmlI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI Tru9I Tsp45I
Tsp509I TspRI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AhdI 433 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 624 GGCGCGCC GG^CGCGCC
AspEI 433 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 478 GGATCC G^GATCC
BanI 580 GGCGCC G^G[CT][GA]CC
BanII 116 GGGCTC G[GA]GC[CT]^C
BbeI 580 GGCGCC GGCGC^C
BfaI 332 CTAG C^TAG
BmyI 116 GGGCTC G[AGT]GC[ACT]^C
BsaI 426 GGTCTC GGTCTC(1/5)
BsgI 17 CTGCAC GTGCAG(16/14)
BsmAI 427 GTCTC GTCTC(1/5)
BsmI 90 GCATTC GAATGC(1/-1)
Bsp1286I 116 GGGCTC G[AGT]GC[ACT]^C
BssSI 454 CTCGTG CACGAG(-5/-1)
BstF5I 444 GGATG GGATG(2/0)
DsaI 317 CCACGG C^C[GA][CT]GG
Eam1105I 433 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 444 GGATG GGATG(9/13)
HaeII 580 GGCGCC [GA]GCGC^[CT]
HphI 740 TCACC GGTGA(8/7)
KasI 580 GGCGCC G^GCGCC
MaeI 332 CTAG C^TAG
NaeI 583 GCCGGC GCC^GGC
NarI 580 GGCGCC GG^CGCC
NdeI 356 CATATG CA^TATG
NgoMIV 583 GCCGGC G^CCGGC
NheI 331 GCTAGC G^CTAGC
NspI 548 ACATGC [GA]CATG^[CT]
SbfI 253 CCTGCAGG CCTGCA^GG
SfoI 580 GGCGCC GGC^GCC
SgrAI 582 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 291 CCCGGG CCC^GGG
Sse8387I 253 CCTGCAGG CCTGCA^GG
TfiI 106 GATTC G^A[AT]TC
XmaCI 291 CCCGGG C^CCGGG
XmaI 291 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaI 291 CCCGGG C^[CT]CG[GA]G
466 CCCGAG
BseRI 600 CTCCTC GAGGAG(10/8)
388 GAGGAG
BsoBI 291 CCCGGG C^[CT]CG[GA]G
466 CCCGAG
BsrFI 583 GCCGGC [GA]^CCGG[CT]
709 ACCGGC
BstYI 478 GGATCC [GA]^GATC[CT]
716 AGATCC
Cfr10I 583 GCCGGC [GA]^CCGG[CT]
709 ACCGGC
DpnII 479 GATC ^GATC
717 GATC
FauI 307 CCCGC CCCGC(4/6)
542 GCGGG
MboI 479 GATC ^GATC
717 GATC
MflI 478 GGATCC [GA]^GATC[CT]
716 AGATCC
NdeII 479 GATC ^GATC
717 GATC
NlaIII 549 CATG CATG^
663 CATG
PflMI 336 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCAGAAGCTGG
PstI 254 CTGCAG CTGCA^G
770 CTGCAG
PvuII 42 CAGCTG CAG^CTG
81 CAGCTG
Sau3AI 479 GATC ^GATC
717 GATC
Van91I 336 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCAGAAGCTGG
XhoII 478 GGATCC [GA]^GATC[CT]
716 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI BbsI BbvI BcnI
BglI BpmI BpuAI BsaHI BsaJI
BsiYI BslI BsmFI BsrI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV PleI RsaI
Sau96I ScrFI SfcI TaqI TseI
TthHB8I