Restriction map for
pFN21AE5146
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaBI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DrdI DsaI EarI Eco47III EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfaNI SfcI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tsp509I TspRI Tth111I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccI 223 GTCTAC GT^[AC][GT]AC
AcyI 153 GGCGCC G[GA]^CG[CT]C
AfaI 336 GTAC GT^AC
AgeI 310 ACCGGT A^CCGGT
AhdI 398 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 138 GGATC GGATC(4/5)
AlwNI 142 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 276 CCTGAGG CC^T[ACGT]AGG
AspEI 398 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 118 CCCGGG C^[CT]CG[GA]G
AvaII 179 GGACC G^G[AT]CC
BalI 354 TGGCCA TGG^CCA
BanI 153 GGCGCC G^G[CT][GA]CC
BbeI 153 GGCGCC GGCGC^C
BbsI 243 GAAGAC GAAGAC(2/6)
BpmI 88 CTGGAG CTGGAG(16/14)
BpuAI 243 GAAGAC GAAGAC(2/6)
BsaAI 286 TACGTG [CT]AC^GT[GA]
BsaHI 153 GGCGCC G[GA]^CG[CT]C
BsaI 91 GAGACC GGTCTC(1/5)
BsiEI 392 CGGCCG CG[GA][CT]^CG
BsmAI 91 GAGAC GTCTC(1/5)
BsoBI 118 CCCGGG C^[CT]CG[GA]G
BspLU11I 20 ACATGT A^CATGT
BsrFI 310 ACCGGT [GA]^CCGG[CT]
BstAPI 188 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 313 GGTGACC G^GT[ACGT]ACC
BstPI 313 GGTGACC G^GT[ACGT]ACC
Bsu36I 276 CCTGAGG CC^T[ACGT]AGG
Cfr10I 310 ACCGGT [GA]^CCGG[CT]
DraIII 283 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 392 CGGCCG C^GGCCG
Eam1105I 398 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 392 CGGCCG C^GGCCG
Eco52I 392 CGGCCG C^GGCCG
Eco81I 276 CCTGAGG CC^T[ACGT]AGG
EcoNI 42 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 313 GGTGACC G^GT[ACGT]ACC
HgaI 398 GACGC GACGC(5/10)
Hin1I 153 GGCGCC G[GA]^CG[CT]C
HinfI 211 GAGTC G^A[ACGT]TC
HphI 313 GGTGA GGTGA(8/7)
KasI 153 GGCGCC G^GCGCC
MaeIII 314 GTGAC ^GT[ACGT]AC
MboII 243 GAAGA GAAGA(8/7)
MluNI 354 TGGCCA TGG^CCA
MscI 354 TGGCCA TGG^CCA
MseI 347 TTAA T^TAA
NarI 153 GGCGCC GG^CGCC
NlaIII 21 CATG CATG^
NspI 20 ACATGT [GA]CATG^[CT]
PinAI 310 ACCGGT A^CCGGT
PleI 211 GAGTC GAGTC(4/5)
PpuMI 178 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 336 GTAC GT^AC
ScaI 335 AGTACT AGT^ACT
SfoI 153 GGCGCC GGC^GCC
SgrAI 309 CACCGGTG C[GA]^CCGG[CT]G
SmaI 118 CCCGGG CCC^GGG
SmlI 331 CTCAAG C^T[CT][GA]AG
TaqI 17 TCGA T^CGA
Tru9I 347 TTAA T^TAA
Tsp45I 314 GTGAC ^GT[GC]AC
TthHB8I 17 TCGA T^CGA
XcmI 164 CCAACGGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 118 CCCGGG C^CCGGG
XmaI 118 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 20 ACATGT A^C[GA][CT]GT
287 ACGTGT
AluI 5 AGCT AG^CT
239 AGCT
BcnI 118 CCCGG CC^[GC]GG
119 CCGGG
BsaWI 249 ACCGGA [AT]^CCGG[AT]
310 ACCGGT
BseRI 228 CTCCTC GAGGAG(10/8)
106 GAGGAG
BstF5I 273 CATCC GGATG(2/0)
252 GGATG
DdeI 43 CTGAG C^T[ACGT]AG
277 CTGAG
DpnII 66 GATC ^GATC
139 GATC
DraII 170 GGGGCCT [GA]G^G[ACGT]CC[CT]
178 GGGACCC
EaeI 354 TGGCCA [CT]^GGCC[GA]
392 CGGCCG
EcoO109I 170 GGGGCCT [GA]G^G[ACGT]CC[CT]
178 GGGACCC
FokI 273 CATCC GGATG(9/13)
252 GGATG
HaeII 60 GGCGCT [GA]GCGC^[CT]
153 GGCGCC
MaeII 221 ACGT A^CGT
287 ACGT
MboI 66 GATC ^GATC
139 GATC
NciI 118 CCCGG CC^[GC]GG
119 CCGGG
NdeII 66 GATC ^GATC
139 GATC
PvuII 4 CAGCTG CAG^CTG
238 CAGCTG
Sau3AI 66 GATC ^GATC
139 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BsmFI BssKI BstNI Cac8I CfoI
Cfr13I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MwoI
NlaIV Sau96I ScrFI TseI