Restriction map for
pFN21AE2086
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# NO CUT ENZYMES ( 78 enzymes) #
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Acc65I AclI AflII AflIII Aor51HI
ApaI AscI AseI AsnI Asp718I
AviII AvrII BbrPI BbvCI BfrI
BglII BlnI BsaBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DraI
DraIII DrdI Eco47III EcoNI EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 385 GACGTC GACGT^C
AccIII 1550 TCCGGA T^CCGGA
AgeI 770 ACCGGT A^CCGGT
AhdI 655 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1831 GTGCAC G^TGCAC
ApaLI 1831 GTGCAC G^TGCAC
Asp700I 253 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 655 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 554 GGCGCC GGCGC^C
BcgI 680 GCAAGGCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 148 GTATCC GTATCC(6/5)
BfaI 2230 CTAG C^TAG
BsaAI 1015 TACGTG [CT]AC^GT[GA]
BseAI 1550 TCCGGA T^CCGGA
BseRI 2239 GAGGAG GAGGAG(10/8)
BsiWI 38 CGTACG C^GTACG
BsmI 1892 GCATTC GAATGC(1/-1)
BspEI 1550 TCCGGA T^CCGGA
BspMI 1916 ACCTGC ACCTGC(4/8)
BstBI 260 TTCGAA TT^CGAA
BstEII 198 GGTGACC G^GT[ACGT]ACC
BstPI 198 GGTGACC G^GT[ACGT]ACC
DraII 542 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 655 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 253 GAAGAG CTCTTC(1/4)
EcoO109I 542 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 198 GGTGACC G^GT[ACGT]ACC
HincII 745 GTCAAC GT[CT]^[GA]AC
HindII 745 GTCAAC GT[CT]^[GA]AC
KasI 554 GGCGCC G^GCGCC
Ksp632I 253 GAAGAG CTCTTC(1/4)
KspI 1773 CCGCGG CCGC^GG
MaeI 2230 CTAG C^TAG
MroI 1550 TCCGGA T^CCGGA
NarI 554 GGCGCC GG^CGCC
NspV 260 TTCGAA TT^CGAA
PinAI 770 ACCGGT A^CCGGT
SacII 1773 CCGCGG CCGC^GG
SapI 253 GAAGAGC GCTCTTC(1/4)
ScaI 2153 AGTACT AGT^ACT
SfiI 675 GGCCCGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 554 GGCGCC GGC^GCC
SfuI 260 TTCGAA TT^CGAA
SphI 600 GCATGC GCATG^C
StuI 1757 AGGCCT AGG^CCT
XcmI 1730 CCAGTGTCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 253 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 28 GTCTAC GT^[AC][GT]AC
1876 GTCTAC
AcsI 478 GAATTT [GA]^AATT[CT]
1519 AAATTT
ApoI 478 GAATTT [GA]^AATT[CT]
1519 AAATTT
AspI 718 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1377 GACACTGTC
BamHI 1667 GGATCC G^GATCC
2087 GGATCC
BclI 164 TGATCA T^GATCA
1478 TGATCA
BlpI 2214 GCTCAGC GC^T[ACGT]AGC
2269 GCTCAGC
Bpu1102I 2214 GCTCAGC GC^T[ACGT]AGC
2269 GCTCAGC
BsiEI 974 CGGCCG CG[GA][CT]^CG
1485 CGGCCG
BsmBI 898 GAGACG CGTCTC(1/5)
2242 GAGACG
BsrBI 1186 CCGCTC CCGCTC(-3/-3)
1074 GAGCGG
BsrDI 1281 CATTGC GCAATG(2/0)
1493 GCAATG
BsrFI 770 ACCGGT [GA]^CCGG[CT]
854 ACCGGC
BstAPI 834 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1041 GCATGTGGTGC
CelII 2214 GCTCAGC GC^T[ACGT]AGC
2269 GCTCAGC
Cfr10I 770 ACCGGT [GA]^CCGG[CT]
854 ACCGGC
EagI 974 CGGCCG C^GGCCG
1485 CGGCCG
EclXI 974 CGGCCG C^GGCCG
1485 CGGCCG
Eco52I 974 CGGCCG C^GGCCG
1485 CGGCCG
FbaI 164 TGATCA T^GATCA
1478 TGATCA
MslI 490 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
601 CATGCCAATG
NcoI 489 CCATGG C^CATGG
1430 CCATGG
PflFI 718 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1377 GACACTGTC
PflMI 1626 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1983 CCAGTGTGTGG
PleI 733 GACTC GAGTC(4/5)
113 GAGTC
PvuII 1305 CAGCTG CAG^CTG
2197 CAGCTG
SacI 79 GAGCTC GAGCT^C
327 GAGCTC
SexAI 656 ACCTGGT A^CC[AT]GGT
1499 ACCAGGT
Tsp509I 479 AATT ^AATT
1520 AATT
Tth111I 718 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1377 GACACTGTC
Van91I 1626 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1983 CCAGTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI Eco81I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII
XmaCI XmaI