Restriction map for
pFN21AE1869
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbsI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BseAI BseRI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III EcoNI
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI Ksp632I
MaeI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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Acc65I 429 GGTACC G^GTACC
AocI 295 CCTGAGG CC^T[ACGT]AGG
Asp718I 429 GGTACC G^GTACC
BamHI 929 GGATCC G^GATCC
BbrPI 499 CACGTG CAC^GTG
BsmFI 84 GGGAC GGGAC(10/14)
BspMI 94 ACCTGC ACCTGC(4/8)
BsrI 54 ACTGG ACTGG(1/-1)
BssSI 934 CACGAG CACGAG(-5/-1)
BstF5I 144 CATCC GGATG(2/0)
BstXI 785 CCACCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
FokI 144 CATCC GGATG(9/13)
HinfI 571 GACTC G^A[ACGT]TC
KpnI 429 GGTACC GGTAC^C
MseI 484 TTAA T^TAA
PleI 571 GACTC GAGTC(4/5)
PmaCI 499 CACGTG CAC^GTG
PmlI 499 CACGTG CAC^GTG
SacI 415 GAGCTC GAGCT^C
SmlI 478 CTCAAG C^T[CT][GA]AG
Tru9I 484 TTAA T^TAA
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# TWO CUT ENZYMES ( 40 enzymes) #
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ApaI 800 GGGCCC GGGCC^C
970 GGGCCC
AspHI 229 GTGCTC G[AT]GC[AT]^C
415 GAGCTC
AvaII 912 GGACC G^G[AT]CC
1014 GGTCC
BalI 740 TGGCCA TGG^CCA
782 TGGCCA
BbeI 1045 GGCGCC GGCGC^C
1056 GGCGCC
BbvCI 543 CCTCAGC CCTCAGC(-5/-2)
471 GCTGAGG
BcgI 98 GCAAGACTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
908 GCAAGGACCTCG
BpmI 793 CTGGAG CTGGAG(16/14)
1081 CTGGAG
BsaAI 499 CACGTG [CT]AC^GT[GA]
1105 TACGTG
BsaWI 282 ACCGGA [AT]^CCGG[AT]
601 ACCGGA
BsgI 275 GTGCAG GTGCAG(16/14)
1164 GTGCAG
BsiHKAI 229 GTGCTC G[AT]GC[AT]^C
415 GAGCTC
BstEII 321 GGTCACC G^GT[ACGT]ACC
733 GGTGACC
BstPI 321 GGTCACC G^GT[ACGT]ACC
733 GGTGACC
BstYI 155 AGATCC [GA]^GATC[CT]
929 GGATCC
EcoO65I 321 GGTCACC G^GT[ACGT]ACC
733 GGTGACC
FseI 70 GGCCGGCC GGCCGG^CC
74 GGCCGGCC
HgaI 63 GACGC GACGC(5/10)
465 GCGTC
KasI 1045 GGCGCC G^GCGCC
1056 GGCGCC
KspI 80 CCGCGG CCGC^GG
147 CCGCGG
MaeII 500 ACGT A^CGT
1106 ACGT
MboII 870 GAAGA GAAGA(8/7)
401 TCTTC
MflI 155 AGATCC [GA]^GATC[CT]
929 GGATCC
MluNI 740 TGGCCA TGG^CCA
782 TGGCCA
MscI 740 TGGCCA TGG^CCA
782 TGGCCA
NaeI 71 GCCGGC GCC^GGC
75 GCCGGC
NarI 1045 GGCGCC GG^CGCC
1056 GGCGCC
NgoMIV 71 GCCGGC G^CCGGC
75 GCCGGC
PpuMI 911 AGGACCT [GA]G^G[AT]CC[CT]
1013 AGGTCCT
PvuII 889 CAGCTG CAG^CTG
1132 CAGCTG
SacII 80 CCGCGG CCGC^GG
147 CCGCGG
SfaNI 134 GCATC GCATC(5/9)
807 GCATC
SfoI 1045 GGCGCC GGC^GCC
1056 GGCGCC
SmaI 452 CCCGGG CCC^GGG
1041 CCCGGG
TaqI 107 TCGA T^CGA
137 TCGA
Tsp509I 529 AATT ^AATT
639 AATT
TthHB8I 107 TCGA T^CGA
137 TCGA
XhoII 155 AGATCC [GA]^GATC[CT]
929 GGATCC
XmaCI 452 CCCGGG C^CCGGG
1041 CCCGGG
XmaI 452 CCCGGG C^CCGGG
1041 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI BanI BanII
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiEI BsiYI BslI BsoBI
Bsp1286I Bsp1407I BsrFI BsrGI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI MaeIII MboI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SspBI StyI TseI Tsp45I TspRI