##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AciI          349  CCGC                 CCGC(-3/-1)
AfaI          283  GTAC                 GT^AC
Alw44I        166  GTGCAC               G^TGCAC
ApaLI         166  GTGCAC               G^TGCAC
AspHI         166  GTGCAC               G[AT]GC[AT]^C
BbsI            3  GTCTTC               GAAGAC(2/6)
BbvI          105  GCTGC                GCAGC(8/12)
BpuAI           3  GTCTTC               GAAGAC(2/6)
BseRI         223  GAGGAG               GAGGAG(10/8)
BsiHKAI       166  GTGCAC               G[AT]GC[AT]^C
BsmAI         226  GAGAC                GTCTC(1/5)
BsmFI          70  GGGAC                GGGAC(10/14)
BspHI         188  TCATGA               T^CATGA
BspMI         108  GCAGGT               ACCTGC(4/8)
DdeI          399  CTCAG                C^T[ACGT]AG
EcoRV         385  GATATC               GAT^ATC
FauI          348  CCCGC                CCCGC(4/6)
Fnu4HI        105  GCTGC                GC^[ACGT]GC
ItaI          105  GCTGC                GC^[ACGT]GC
MspA1I        103  CAGCTG               C[AC]G^C[GT]G
MwoI          323  GCATTTACGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV          49  GGTTCC               GG[ACGT]^[ACGT]CC
PstI          106  CTGCAG               CTGCA^G
PvuII         103  CAGCTG               CAG^CTG
RcaI          188  TCATGA               T^CATGA
RsaI          283  GTAC                 GT^AC
SfaNI         275  GCATC                GCATC(5/9)
SfcI          106  CTGCAG               C^T[GA][CT]AG
SnaBI         280  TACGTA               TAC^GTA
TseI          105  GCTGC                G^C[AT]GC
Tsp509I       263  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          104  AGCT                 AG^CT
              269  AGCT                
BmyI          166  GTGCAC               G[AGT]GC[ACT]^C
              241  GTGCCC              
BsaAI         238  TACGTG               [CT]AC^GT[GA]
              280  TACGTA              
Bsp1286I      166  GTGCAC               G[AGT]GC[ACT]^C
              241  GTGCCC              
HinfI         139  GATTC                G^A[ACGT]TC
              344  GAATC               
MaeII         239  ACGT                 A^CGT
              281  ACGT                
MboII         363  GAAGA                GAAGA(8/7)
                4  TCTTC               
MslI          123  CACTGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              286  CATTACAATG          
TaqI          342  TCGA                 T^CGA
              379  TCGA                
TfiI          139  GATTC                G^A[AT]TC
              344  GAATC               
TspRI         123  CACTG                CA[GC]TG[ACGT][ACGT]^
              401  CAGTG               
TthHB8I       342  TCGA                 T^CGA
              379  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BstF5I      FokI        MnlI        NlaIII