Restriction map for
pFN21AE1585
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MscI MseI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 250 GTCTAC GT^[AC][GT]AC
AccIII 118 TCCGGA T^CCGGA
AluI 51 AGCT AG^CT
AlwI 3 GATCC GGATC(4/5)
AlwNI 41 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 128 GGTCC G^G[AT]CC
AviII 211 TGCGCA TGC^GCA
BpmI 62 CTCCAG CTGGAG(16/14)
BsaJI 96 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 118 TCCGGA [AT]^CCGG[AT]
BseAI 118 TCCGGA T^CCGGA
BsmFI 129 GTCCC GGGAC(10/14)
BspEI 118 TCCGGA T^CCGGA
BsrBI 142 CCGCTC CCGCTC(-3/-3)
BsrI 40 CCAGT ACTGG(1/-1)
BssSI 157 CTCGTG CACGAG(-5/-1)
CfoI 212 GCGC GCG^C
Cfr13I 128 GGTCC G^G[ACGT]CC
DdeI 53 CTCAG C^T[ACGT]AG
DraII 127 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 22 CTCTTC CTCTTC(1/4)
EcoO109I 127 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 232 CCCGC CCCGC(4/6)
FspI 211 TGCGCA TGC^GCA
HaeIII 154 GGCC GG^CC
HapII 119 CCGG C^CGG
HhaI 212 GCGC GCG^C
HinP1I 212 GCGC G^CGC
HinfI 60 GACTC G^A[ACGT]TC
HpaII 119 CCGG C^CGG
Ksp632I 22 CTCTTC CTCTTC(1/4)
MaeIII 14 GTCAC ^GT[ACGT]AC
MboII 23 TCTTC GAAGA(8/7)
MroI 118 TCCGGA T^CCGGA
MslI 255 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 119 CCGG C^CGG
NlaIII 261 CATG CATG^
NspI 260 ACATGC [GA]CATG^[CT]
PleI 60 GACTC GAGTC(4/5)
PpuMI 127 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 47 CTGCAG CTGCA^G
Sau96I 128 GGTCC G^G[ACGT]CC
SfcI 47 CTGCAG C^T[GA][CT]AG
StuI 153 AGGCCT AGG^CCT
Tsp45I 14 GTCAC ^GT[GC]AC
TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 11 enzymes) #
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BsiYI 119 CCGGATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
265 CCAGGCAGGGG
BslI 119 CCGGATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
265 CCAGGCAGGGG
BstF5I 18 CATCC GGATG(2/0)
121 GGATG
Cac8I 151 GCAGGC GC[ACGT]^[ACGT]GC
214 GCACGC
DpnII 3 GATC ^GATC
74 GATC
FokI 18 CATCC GGATG(9/13)
121 GGATG
MboI 3 GATC ^GATC
74 GATC
NdeII 3 GATC ^GATC
74 GATC
NlaIV 127 GGGTCC GG[ACGT]^[ACGT]CC
128 GGTCCC
Sau3AI 3 GATC ^GATC
74 GATC
SfaNI 169 GATGC GCATC(5/9)
178 GCATC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AciI BbvI BssKI BstNI EcoRII
Fnu4HI ItaI MnlI MvaI MwoI
ScrFI TseI