################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MscI MseI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 250 GTCTAC GT^[AC][GT]AC AccIII 118 TCCGGA T^CCGGA AluI 51 AGCT AG^CT AlwI 3 GATCC GGATC(4/5) AlwNI 41 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 128 GGTCC G^G[AT]CC AviII 211 TGCGCA TGC^GCA BpmI 62 CTCCAG CTGGAG(16/14) BsaJI 96 CCCTGG C^C[ACGT][ACGT]GG BsaWI 118 TCCGGA [AT]^CCGG[AT] BseAI 118 TCCGGA T^CCGGA BsmFI 129 GTCCC GGGAC(10/14) BspEI 118 TCCGGA T^CCGGA BsrBI 142 CCGCTC CCGCTC(-3/-3) BsrI 40 CCAGT ACTGG(1/-1) BssSI 157 CTCGTG CACGAG(-5/-1) CfoI 212 GCGC GCG^C Cfr13I 128 GGTCC G^G[ACGT]CC DdeI 53 CTCAG C^T[ACGT]AG DraII 127 GGGTCCC [GA]G^G[ACGT]CC[CT] EarI 22 CTCTTC CTCTTC(1/4) EcoO109I 127 GGGTCCC [GA]G^G[ACGT]CC[CT] FauI 232 CCCGC CCCGC(4/6) FspI 211 TGCGCA TGC^GCA HaeIII 154 GGCC GG^CC HapII 119 CCGG C^CGG HhaI 212 GCGC GCG^C HinP1I 212 GCGC G^CGC HinfI 60 GACTC G^A[ACGT]TC HpaII 119 CCGG C^CGG Ksp632I 22 CTCTTC CTCTTC(1/4) MaeIII 14 GTCAC ^GT[ACGT]AC MboII 23 TCTTC GAAGA(8/7) MroI 118 TCCGGA T^CCGGA MslI 255 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 119 CCGG C^CGG NlaIII 261 CATG CATG^ NspI 260 ACATGC [GA]CATG^[CT] PleI 60 GACTC GAGTC(4/5) PpuMI 127 GGGTCCC [GA]G^G[AT]CC[CT] PstI 47 CTGCAG CTGCA^G Sau96I 128 GGTCC G^G[ACGT]CC SfcI 47 CTGCAG C^T[GA][CT]AG StuI 153 AGGCCT AGG^CCT Tsp45I 14 GTCAC ^GT[GC]AC TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsiYI 119 CCGGATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 265 CCAGGCAGGGG BslI 119 CCGGATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 265 CCAGGCAGGGG BstF5I 18 CATCC GGATG(2/0) 121 GGATG Cac8I 151 GCAGGC GC[ACGT]^[ACGT]GC 214 GCACGC DpnII 3 GATC ^GATC 74 GATC FokI 18 CATCC GGATG(9/13) 121 GGATG MboI 3 GATC ^GATC 74 GATC NdeII 3 GATC ^GATC 74 GATC NlaIV 127 GGGTCC GG[ACGT]^[ACGT]CC 128 GGTCCC Sau3AI 3 GATC ^GATC 74 GATC SfaNI 169 GATGC GCATC(5/9) 178 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AciI BbvI BssKI BstNI EcoRII Fnu4HI ItaI MnlI MvaI MwoI ScrFI TseI