Restriction map for
pFN21AE6030
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AflII AgeI AhdI
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmFI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspA1I MunI MvnI NarI
NciI NcoI NdeI NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 817 GACGCC G[GA]^CG[CT]C
AflIII 724 ACGTGT A^C[GA][CT]GT
AluI 893 AGCT AG^CT
Alw44I 192 GTGCAC G^TGCAC
AlwI 522 GATCC GGATC(4/5)
AocI 879 CCTAAGG CC^T[ACGT]AGG
ApaLI 192 GTGCAC G^TGCAC
BanII 411 GAGCCC G[GA]GC[CT]^C
BbvI 673 GCTGC GCAGC(8/12)
BciVI 730 GTATCC GTATCC(6/5)
BfaI 5 CTAG C^TAG
BglI 820 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 723 TACGTG [CT]AC^GT[GA]
BsaHI 817 GACGCC G[GA]^CG[CT]C
BsmAI 205 GTCTC GTCTC(1/5)
BsmBI 204 CGTCTC CGTCTC(1/5)
BsmI 900 GAATGC GAATGC(1/-1)
Bsp1407I 809 TGTACA T^GTACA
BspHI 407 TCATGA T^CATGA
BsrDI 116 CATTGC GCAATG(2/0)
BsrFI 704 GCCGGC [GA]^CCGG[CT]
BsrGI 809 TGTACA T^GTACA
BstAPI 66 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 879 CCTAAGG CC^T[ACGT]AGG
Cfr10I 704 GCCGGC [GA]^CCGG[CT]
Cfr13I 795 GGGCC G^G[ACGT]CC
DraII 794 AGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 184 CTCTTC CTCTTC(1/4)
Eco81I 879 CCTAAGG CC^T[ACGT]AGG
EcoO109I 794 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 791 CCAAGG C^C[AT][AT]GG
Fnu4HI 673 GCTGC GC^[ACGT]GC
HapII 705 CCGG C^CGG
HgaI 817 GACGC GACGC(5/10)
Hin1I 817 GACGCC G[GA]^CG[CT]C
HpaII 705 CCGG C^CGG
ItaI 673 GCTGC GC^[ACGT]GC
Ksp632I 184 CTCTTC CTCTTC(1/4)
MaeI 5 CTAG C^TAG
MaeII 724 ACGT A^CGT
MseI 35 TTAA T^TAA
MspI 705 CCGG C^CGG
NaeI 704 GCCGGC GCC^GGC
NgoMIV 704 GCCGGC G^CCGGC
NspI 242 GCATGC [GA]CATG^[CT]
PleI 164 GACTC GAGTC(4/5)
RcaI 407 TCATGA T^CATGA
Sau96I 795 GGGCC G^G[ACGT]CC
SphI 242 GCATGC GCATG^C
SspBI 809 TGTACA T^GTACA
StyI 791 CCAAGG C^C[AT][AT]GG
Tru9I 35 TTAA T^TAA
TseI 673 GCTGC G^C[AT]GC
XcmI 275 CCATCACCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
928 AAATTC
ApoI 31 GAATTT [GA]^AATT[CT]
928 AAATTC
AspHI 192 GTGCAC G[AT]GC[AT]^C
403 GTGCTC
BbsI 661 GTCTTC GAAGAC(2/6)
844 GTCTTC
BpuAI 661 GTCTTC GAAGAC(2/6)
844 GTCTTC
BsaJI 446 CCTGGG C^C[ACGT][ACGT]GG
791 CCAAGG
BsiHKAI 192 GTGCAC G[AT]GC[AT]^C
403 GTGCTC
BssKI 348 CCTGG ^CC[ACGT]GG
446 CCTGG
BstF5I 648 CATCC GGATG(2/0)
470 GGATG
BstNI 348 CCTGG CC^[AT]GG
446 CCTGG
EcoRII 348 CCTGG ^CC[AT]GG
446 CCTGG
FokI 648 CATCC GGATG(9/13)
470 GGATG
MvaI 348 CCTGG CC^[AT]GG
446 CCTGG
PflMI 143 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
429 CCATCTTGTGG
ScrFI 348 CCTGG CC^[ACGT]GG
446 CCTGG
SfaNI 603 GATGC GCATC(5/9)
20 GCATC
SfcI 15 CTATAG C^T[GA][CT]AG
759 CTATAG
SmlI 681 CTTGAG C^T[CT][GA]AG
784 CTCAAG
TfiI 213 GATTC G^A[AT]TC
687 GATTC
Tsp509I 32 AATT ^AATT
929 AATT
Van91I 143 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
429 CCATCTTGTGG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI BalI BmyI BsiYI BslI
Bsp1286I Cac8I DdeI DpnII EaeI
HaeIII HinfI HphI MaeIII MboI
MboII MluNI MnlI MscI MslI
MwoI NdeII NlaIII RsaI Sau3AI
Tsp45I TspRI