Restriction map for
pFN21AE5392
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 205 GTAGAC GT^[AC][GT]AC
AciI 620 GCGG CCGC(-3/-1)
AgeI 362 ACCGGT A^CCGGT
AlwNI 809 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 609 AGCGCT AGC^GCT
AseI 541 ATTAAT AT^TAAT
AsnI 541 ATTAAT AT^TAAT
AspHI 115 GTGCTC G[AT]GC[AT]^C
AvaI 118 CTCGGG C^[CT]CG[GA]G
BamHI 638 GGATCC G^GATCC
BmyI 115 GTGCTC G[AGT]GC[ACT]^C
BsaAI 674 TACGTG [CT]AC^GT[GA]
BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BsgI 388 CTGCAC GTGCAG(16/14)
BsiHKAI 115 GTGCTC G[AT]GC[AT]^C
BsoBI 118 CTCGGG C^[CT]CG[GA]G
Bsp1286I 115 GTGCTC G[AGT]GC[ACT]^C
BspHI 449 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BsrI 634 ACTGG ACTGG(1/-1)
BstEII 840 GGTCACC G^GT[ACGT]ACC
BstPI 840 GGTCACC G^GT[ACGT]ACC
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 638 GGATCC [GA]^GATC[CT]
CfoI 610 GCGC GCG^C
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
Eco47III 609 AGCGCT AGC^GCT
EcoO65I 840 GGTCACC G^GT[ACGT]ACC
EcoRI 462 GAATTC G^AATTC
HaeII 609 AGCGCT [GA]GCGC^[CT]
HapII 363 CCGG C^CGG
HhaI 610 GCGC GCG^C
HinP1I 610 GCGC G^CGC
HindIII 477 AAGCTT A^AGCTT
HinfI 154 GACTC G^A[ACGT]TC
HpaII 363 CCGG C^CGG
MaeII 675 ACGT A^CGT
MflI 638 GGATCC [GA]^GATC[CT]
MspI 363 CCGG C^CGG
NspI 760 ACATGC [GA]CATG^[CT]
PinAI 362 ACCGGT A^CCGGT
PleI 154 GACTC GAGTC(4/5)
PshBI 541 ATTAAT AT^TAAT
PstI 815 CTGCAG CTGCA^G
RcaI 449 TCATGA T^CATGA
SexAI 104 ACCTGGT A^CC[AT]GGT
StuI 71 AGGCCT AGG^CCT
XhoII 638 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 478 AGCT AG^CT
690 AGCT
AvaII 552 GGTCC G^G[AT]CC
625 GGTCC
BalI 140 TGGCCA TGG^CCA
371 TGGCCA
BsmAI 220 GTCTC GTCTC(1/5)
742 GTCTC
BsmFI 232 GTCCC GGGAC(10/14)
626 GTCCC
Bsp1407I 505 TGTACA T^GTACA
830 TGTACA
BsrGI 505 TGTACA T^GTACA
830 TGTACA
Cac8I 69 GCAGGC GC[ACGT]^[ACGT]GC
283 GCAGGC
Cfr13I 552 GGTCC G^G[ACGT]CC
625 GGTCC
DpnII 447 GATC ^GATC
639 GATC
EaeI 140 TGGCCA [CT]^GGCC[GA]
371 TGGCCA
MboI 447 GATC ^GATC
639 GATC
MluNI 140 TGGCCA TGG^CCA
371 TGGCCA
MscI 140 TGGCCA TGG^CCA
371 TGGCCA
NdeII 447 GATC ^GATC
639 GATC
Sau3AI 447 GATC ^GATC
639 GATC
Sau96I 552 GGTCC G^G[ACGT]CC
625 GGTCC
SfcI 815 CTGCAG C^T[GA][CT]AG
867 CTACAG
SspBI 505 TGTACA T^GTACA
830 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AlwI ApoI Asp700I
BbvI BsiYI BslI BssKI BstF5I
BstNI DdeI EcoRII Fnu4HI FokI
HaeIII HphI ItaI MaeIII MboII
MnlI MseI MslI MvaI NlaIII
NlaIV RsaI ScrFI SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI