Restriction map for
pFN21AE5943
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AvaI AviII
AvrII BbeI BbrPI BbsI BcgI
BclI BcnI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 31 AAATTT [GA]^AATT[CT]
AgeI 362 ACCGGT A^CCGGT
AhdI 764 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 509 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApaI 896 GGGCCC GGGCC^C
ApoI 31 AAATTT [GA]^AATT[CT]
Asp700I 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 764 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 549 GGTCC G^G[AT]CC
BamHI 638 GGATCC G^GATCC
BanI 287 GGTGCC G^G[CT][GA]CC
BanII 896 GGGCCC G[GA]GC[CT]^C
BbvCI 912 CCTCAGC CCTCAGC(-5/-2)
BciVI 330 GGATAC GTATCC(6/5)
BfaI 244 CTAG C^TAG
BmyI 896 GGGCCC G[AGT]GC[ACT]^C
BsaI 741 GGTCTC GGTCTC(1/5)
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BsgI 388 CTGCAC GTGCAG(16/14)
Bsp1286I 896 GGGCCC G[AGT]GC[ACT]^C
BspHI 404 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrI 342 ACTGG ACTGG(1/-1)
BstEII 840 GGTCACC G^GT[ACGT]ACC
BstPI 840 GGTCACC G^GT[ACGT]ACC
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC
Eam1105I 764 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoNI 291 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 840 GGTCACC G^GT[ACGT]ACC
MaeI 244 CTAG C^TAG
NcoI 836 CCATGG C^CATGG
PinAI 362 ACCGGT A^CCGGT
PpuMI 548 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 925 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 67 CTGCAG CTGCA^G
RcaI 404 TCATGA T^CATGA
SexAI 104 ACCTGGT A^CC[AT]GGT
SfiI 345 GGCCGTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 553 CTCAAG C^T[CT][GA]AG
TfiI 519 GATTC G^A[AT]TC
XcmI 605 CCACGGCACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 523 CCGC CCGC(-3/-1)
818 CCGC
BalI 371 TGGCCA TGG^CCA
602 TGGCCA
BbvI 69 GCAGC GCAGC(8/12)
809 GCAGC
BglI 346 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
416 GCCTCTGTGGC
BsmAI 664 GTCTC GTCTC(1/5)
742 GTCTC
BsmFI 45 GGGAC GGGAC(10/14)
232 GTCCC
BsrFI 362 ACCGGT [GA]^CCGG[CT]
540 ACCGGC
BstF5I 252 CATCC GGATG(2/0)
885 GGATG
BstYI 638 GGATCC [GA]^GATC[CT]
724 GGATCT
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
540 ACCGGC
DsaI 605 CCACGG C^C[GA][CT]GG
836 CCATGG
EarI 643 CTCTTC CTCTTC(1/4)
489 GAAGAG
EcoT14I 692 CCAAGG C^C[AT][AT]GG
836 CCATGG
Fnu4HI 69 GCAGC GC^[ACGT]GC
809 GCAGC
FokI 252 CATCC GGATG(9/13)
885 GGATG
HapII 363 CCGG C^CGG
541 CCGG
HincII 205 GTTGAC GT[CT]^[GA]AC
495 GTTGAC
HindII 205 GTTGAC GT[CT]^[GA]AC
495 GTTGAC
HpaII 363 CCGG C^CGG
541 CCGG
HphI 729 TCACC GGTGA(8/7)
842 TCACC
ItaI 69 GCAGC GC^[ACGT]GC
809 GCAGC
Ksp632I 643 CTCTTC CTCTTC(1/4)
489 GAAGAG
MflI 638 GGATCC [GA]^GATC[CT]
724 GGATCT
MluNI 371 TGGCCA TGG^CCA
602 TGGCCA
MscI 371 TGGCCA TGG^CCA
602 TGGCCA
MseI 310 TTAA T^TAA
673 TTAA
MspI 363 CCGG C^CGG
541 CCGG
MwoI 346 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
416 GCCTCTGTGGC
StyI 692 CCAAGG C^C[AT][AT]GG
836 CCATGG
Tru9I 310 TTAA T^TAA
673 TTAA
TseI 69 GCAGC G^C[AT]GC
809 GCAGC
Tsp509I 23 AATT ^AATT
32 AATT
XhoII 638 GGATCC [GA]^GATC[CT]
724 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BseRI
BsiYI BslI BssKI BstNI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII HaeIII HinfI MaeIII MboI
MboII MnlI MslI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI Tsp45I
TspRI