Restriction map for
pFN21AE5939
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmAI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
AclI 511 AACGTT AA^CGTT
AcsI 31 GAATTT [GA]^AATT[CT]
AlwI 240 GATCC GGATC(4/5)
AlwNI 475 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTT [GA]^AATT[CT]
AspHI 453 GAGCAC G[AT]GC[AT]^C
BalI 371 TGGCCA TGG^CCA
BbsI 798 GTCTTC GAAGAC(2/6)
BfaI 244 CTAG C^TAG
BglII 299 AGATCT A^GATCT
BmyI 453 GAGCAC G[AGT]GC[ACT]^C
BpuAI 798 GTCTTC GAAGAC(2/6)
BsiEI 360 CGACCG CG[GA][CT]^CG
BsiHKAI 453 GAGCAC G[AT]GC[AT]^C
BsmI 680 GAATGC GAATGC(1/-1)
Bsp1286I 453 GAGCAC G[AGT]GC[ACT]^C
BspMI 125 ACCTGC ACCTGC(4/8)
BstEII 108 GGTCACC G^GT[ACGT]ACC
BstPI 108 GGTCACC G^GT[ACGT]ACC
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoO65I 108 GGTCACC G^GT[ACGT]ACC
HindIII 896 AAGCTT A^AGCTT
MaeI 244 CTAG C^TAG
MaeII 512 ACGT A^CGT
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MwoI 346 GCAGTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
Psp1406I 511 AACGTT AA^CGTT
SexAI 104 ACCTGGT A^CC[AT]GGT
SfaNI 463 GATGC GCATC(5/9)
SfcI 867 CTACAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbvI 492 GCAGC GCAGC(8/12)
287 GCTGC
BsaJI 117 CCTGGG C^C[ACGT][ACGT]GG
467 CCCTGG
Bsp1407I 773 TGTACA T^GTACA
830 TGTACA
BsrGI 773 TGTACA T^GTACA
830 TGTACA
BstYI 239 AGATCC [GA]^GATC[CT]
299 AGATCT
DpnII 240 GATC ^GATC
300 GATC
DraII 3 GGGACCC [GA]G^G[ACGT]CC[CT]
56 AGGACCC
EcoO109I 3 GGGACCC [GA]G^G[ACGT]CC[CT]
56 AGGACCC
Fnu4HI 287 GCTGC GC^[ACGT]GC
492 GCAGC
HaeIII 354 GGCC GG^CC
372 GGCC
ItaI 287 GCTGC GC^[ACGT]GC
492 GCAGC
MboI 240 GATC ^GATC
300 GATC
MflI 239 AGATCC [GA]^GATC[CT]
299 AGATCT
MspA1I 20 CAGCTG C[AC]G^C[GT]G
493 CAGCTG
NdeII 240 GATC ^GATC
300 GATC
PpuMI 3 GGGACCC [GA]G^G[AT]CC[CT]
56 AGGACCC
PvuII 20 CAGCTG CAG^CTG
493 CAGCTG
Sau3AI 240 GATC ^GATC
300 GATC
SspBI 773 TGTACA T^GTACA
830 TGTACA
TfiI 264 GAATC G^A[AT]TC
432 GATTC
TseI 287 GCTGC G^C[AT]GC
492 GCAGC
XhoII 239 AGATCC [GA]^GATC[CT]
299 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI Asp700I AvaII BsiYI
BslI BsmFI BspHI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FokI HinfI HphI MaeIII MboII
MnlI MseI MslI MvaI NlaIII
NlaIV PleI RcaI RsaI Sau96I
ScrFI Tru9I Tsp45I Tsp509I TspRI
XmnI