Restriction map for
pFN21AE2440
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# NO CUT ENZYMES ( 39 enzymes) #
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AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I AspEI BsiWI
BsrBI BssHII Bst1107I BstZ17I CpoI
Eam1105I EcoRV EcoT22I FseI MluI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SapI SbfI SfiI SnaBI SpeI
Sse8387I SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 7457 TCCGGA T^CCGGA
AclI 1426 AACGTT AA^CGTT
AflII 336 CTTAAG C^TTAAG
AocI 3279 CCTGAGG CC^T[ACGT]AGG
Aor51HI 619 AGCGCT AGC^GCT
ApaI 1405 GGGCCC GGGCC^C
AvrII 8511 CCTAGG C^CTAGG
BamHI 747 GGATCC G^GATCC
BbrPI 7621 CACGTG CAC^GTG
BbsI 6910 GAAGAC GAAGAC(2/6)
BciVI 6608 GTATCC GTATCC(6/5)
BfrI 336 CTTAAG C^TTAAG
BglII 708 AGATCT A^GATCT
BlnI 8511 CCTAGG C^CTAGG
BpuAI 6910 GAAGAC GAAGAC(2/6)
BsaBI 4995 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 7457 TCCGGA T^CCGGA
BspDI 7210 ATCGAT AT^CGAT
BspEI 7457 TCCGGA T^CCGGA
BspHI 713 TCATGA T^CATGA
Bsu36I 3279 CCTGAGG CC^T[ACGT]AGG
ClaI 7210 ATCGAT AT^CGAT
DrdI 8836 GACGGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 619 AGCGCT AGC^GCT
Eco81I 3279 CCTGAGG CC^T[ACGT]AGG
HpaI 8596 GTTAAC GTT^AAC
MamI 4995 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 7457 TCCGGA T^CCGGA
PmaCI 7621 CACGTG CAC^GTG
PmlI 7621 CACGTG CAC^GTG
Psp1406I 1426 AACGTT AA^CGTT
RcaI 713 TCATGA T^CATGA
ScaI 1697 AGTACT AGT^ACT
SgrAI 2292 CACCGGCG C[GA]^CCGG[CT]G
SphI 1818 GCATGC GCATG^C
SspI 3884 AATATT AAT^ATT
XbaI 5490 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AviII 2280 TGCGCA TGC^GCA
9089 TGCGCA
BbeI 1643 GGCGCC GGCGC^C
2635 GGCGCC
BcgI 1956 GCAGATTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
9170 GCAGAGCGTTCG
BsaAI 3493 TACGTG [CT]AC^GT[GA]
7621 CACGTG
BssSI 3142 CTCGTG CACGAG(-5/-1)
5299 CTCGTG
BstBI 8759 TTCGAA TT^CGAA
9178 TTCGAA
DraI 5028 TTTAAA TTT^AAA
6802 TTTAAA
EagI 5828 CGGCCG C^GGCCG
8442 CGGCCG
EclXI 5828 CGGCCG C^GGCCG
8442 CGGCCG
Eco52I 5828 CGGCCG C^GGCCG
8442 CGGCCG
FspI 2280 TGCGCA TGC^GCA
9089 TGCGCA
KasI 1643 GGCGCC G^GCGCC
2635 GGCGCC
KspI 1670 CCGCGG CCGC^GG
9108 CCGCGG
MfeI 3132 CAATTG C^AATTG
7792 CAATTG
MunI 3132 CAATTG C^AATTG
7792 CAATTG
NaeI 50 GCCGGC GCC^GGC
2843 GCCGGC
NarI 1643 GGCGCC GG^CGCC
2635 GGCGCC
NgoMIV 50 GCCGGC G^CCGGC
2843 GCCGGC
NheI 7200 GCTAGC G^CTAGC
9126 GCTAGC
NspV 8759 TTCGAA TT^CGAA
9178 TTCGAA
PshAI 1347 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4429 GACGCCTGTC
SacI 5422 GAGCTC GAGCT^C
6660 GAGCTC
SacII 1670 CCGCGG CCGC^GG
9108 CCGCGG
SfoI 1643 GGCGCC GGC^GCC
2635 GGCGCC
SfuI 8759 TTCGAA TT^CGAA
9178 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AatII Acc65I AccI AccII AciI
AcsI AcyI AfaI AflIII AluI
AlwI AlwNI ApoI Asp718I AspHI
AspI AvaI AvaII BalI BanI
BanII BbvCI BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SalI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI