################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI MaeII MamI MfeI MflI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 1855 GACGCC G[GA]^CG[CT]C AflIII 389 ACGCGT A^C[GA][CT]GT Aor51HI 569 AGCGCT AGC^GCT AspI 982 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 1498 GAAGAC GAAGAC(2/6) BbvCI 1149 GCTGAGG CCTCAGC(-5/-2) BcgI 720 CGAGCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1498 GAAGAC GAAGAC(2/6) BsaHI 1855 GACGCC G[GA]^CG[CT]C BsrDI 735 CATTGC GCAATG(2/0) BstEII 904 GGTGACC G^GT[ACGT]ACC BstPI 904 GGTGACC G^GT[ACGT]ACC DpnII 1869 GATC ^GATC Eco47III 569 AGCGCT AGC^GCT EcoO65I 904 GGTGACC G^GT[ACGT]ACC EcoT14I 1673 CCTTGG C^C[AT][AT]GG Hin1I 1855 GACGCC G[GA]^CG[CT]C HincII 1132 GTCAAC GT[CT]^[GA]AC HindII 1132 GTCAAC GT[CT]^[GA]AC MboI 1869 GATC ^GATC MluI 389 ACGCGT A^CGCGT MslI 1893 CATATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 1869 GATC ^GATC NotI 823 GCGGCCGC GC^GGCCGC NspI 1112 ACATGC [GA]CATG^[CT] PflFI 982 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 968 AGGTCCC [GA]G^G[AT]CC[CT] SacI 1066 GAGCTC GAGCT^C Sau3AI 1869 GATC ^GATC SfiI 1531 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 253 CCCGGG CCC^GGG StyI 1673 CCTTGG C^C[AT][AT]GG TfiI 957 GAATC G^A[AT]TC Tth111I 982 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 746 CCAGGCGCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 253 CCCGGG C^CCGGG XmaI 253 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 1229 GTAC GT^AC 1690 GTAC AspHI 622 GAGCAC G[AT]GC[AT]^C 1066 GAGCTC BsgI 773 GTGCAG GTGCAG(16/14) 1100 GTGCAG BsiHKAI 622 GAGCAC G[AT]GC[AT]^C 1066 GAGCTC BsmAI 1109 GAGAC GTCTC(1/5) 86 GTCTC BspMI 908 ACCTGC ACCTGC(4/8) 1407 GCAGGT BssSI 475 CACGAG CACGAG(-5/-1) 634 CACGAG BstAPI 814 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 999 GCAGGAGCTGC EagI 824 CGGCCG C^GGCCG 1530 CGGCCG EclXI 824 CGGCCG C^GGCCG 1530 CGGCCG Eco52I 824 CGGCCG C^GGCCG 1530 CGGCCG HaeII 569 AGCGCT [GA]GCGC^[CT] 697 AGCGCC PaeR7I 565 CTCGAG C^TCGAG 1222 CTCGAG PflMI 633 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 849 CCAGGTGCTGG RsaI 1229 GTAC GT^AC 1690 GTAC SapI 370 GAAGAGC GCTCTTC(1/4) 1809 GCTCTTC SbfI 831 CCTGCAGG CCTGCA^GG 996 CCTGCAGG Sse8387I 831 CCTGCAGG CCTGCA^GG 996 CCTGCAGG StuI 605 AGGCCT AGG^CCT 1727 AGGCCT Van91I 633 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 849 CCAGGTGCTGG XhoI 565 CTCGAG C^TCGAG 1222 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AluI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PleI PstI PvuII SacII Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I TspRI TthHB8I