Restriction map for
pFN21AE4176
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI MaeII
MamI MfeI MflI MluNI MroI
MscI MseI MunI NarI NcoI
NdeI NheI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tsp509I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 1855 GACGCC G[GA]^CG[CT]C
AflIII 389 ACGCGT A^C[GA][CT]GT
Aor51HI 569 AGCGCT AGC^GCT
AspI 982 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1498 GAAGAC GAAGAC(2/6)
BbvCI 1149 GCTGAGG CCTCAGC(-5/-2)
BcgI 720 CGAGCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1498 GAAGAC GAAGAC(2/6)
BsaHI 1855 GACGCC G[GA]^CG[CT]C
BsrDI 735 CATTGC GCAATG(2/0)
BstEII 904 GGTGACC G^GT[ACGT]ACC
BstPI 904 GGTGACC G^GT[ACGT]ACC
DpnII 1869 GATC ^GATC
Eco47III 569 AGCGCT AGC^GCT
EcoO65I 904 GGTGACC G^GT[ACGT]ACC
EcoT14I 1673 CCTTGG C^C[AT][AT]GG
Hin1I 1855 GACGCC G[GA]^CG[CT]C
HincII 1132 GTCAAC GT[CT]^[GA]AC
HindII 1132 GTCAAC GT[CT]^[GA]AC
MboI 1869 GATC ^GATC
MluI 389 ACGCGT A^CGCGT
MslI 1893 CATATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 1869 GATC ^GATC
NotI 823 GCGGCCGC GC^GGCCGC
NspI 1112 ACATGC [GA]CATG^[CT]
PflFI 982 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 968 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 1066 GAGCTC GAGCT^C
Sau3AI 1869 GATC ^GATC
SfiI 1531 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 253 CCCGGG CCC^GGG
StyI 1673 CCTTGG C^C[AT][AT]GG
TfiI 957 GAATC G^A[AT]TC
Tth111I 982 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 746 CCAGGCGCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 253 CCCGGG C^CCGGG
XmaI 253 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 1229 GTAC GT^AC
1690 GTAC
AspHI 622 GAGCAC G[AT]GC[AT]^C
1066 GAGCTC
BsgI 773 GTGCAG GTGCAG(16/14)
1100 GTGCAG
BsiHKAI 622 GAGCAC G[AT]GC[AT]^C
1066 GAGCTC
BsmAI 1109 GAGAC GTCTC(1/5)
86 GTCTC
BspMI 908 ACCTGC ACCTGC(4/8)
1407 GCAGGT
BssSI 475 CACGAG CACGAG(-5/-1)
634 CACGAG
BstAPI 814 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
999 GCAGGAGCTGC
EagI 824 CGGCCG C^GGCCG
1530 CGGCCG
EclXI 824 CGGCCG C^GGCCG
1530 CGGCCG
Eco52I 824 CGGCCG C^GGCCG
1530 CGGCCG
HaeII 569 AGCGCT [GA]GCGC^[CT]
697 AGCGCC
PaeR7I 565 CTCGAG C^TCGAG
1222 CTCGAG
PflMI 633 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
849 CCAGGTGCTGG
RsaI 1229 GTAC GT^AC
1690 GTAC
SapI 370 GAAGAGC GCTCTTC(1/4)
1809 GCTCTTC
SbfI 831 CCTGCAGG CCTGCA^GG
996 CCTGCAGG
Sse8387I 831 CCTGCAGG CCTGCA^GG
996 CCTGCAGG
StuI 605 AGGCCT AGG^CCT
1727 AGGCCT
Van91I 633 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
849 CCAGGTGCTGG
XhoI 565 CTCGAG C^TCGAG
1222 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwNI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PleI PstI
PvuII SacII Sau96I ScrFI SfaNI
SfcI SmlI TaqI TseI Tsp45I
TspRI TthHB8I