Restriction map for
pFN21AE3477
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII AccI AccII AclI AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI AspI AvrII BbeI BcgI
BclI BglI BlnI BsiEI BsiWI
BsmBI Bsp1407I BspDI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco52I
EcoO65I EcoT22I FbaI FseI HpaI
KasI KspI MluI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 3936 GGTACC G^GTACC
AccIII 1110 TCCGGA T^CCGGA
AcyI 4327 GACGCC G[GA]^CG[CT]C
AflII 3844 CTTAAG C^TTAAG
AflIII 2683 ACATGT A^C[GA][CT]GT
AhdI 4774 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4096 AGCGCT AGC^GCT
Asp718I 3936 GGTACC G^GTACC
AspEI 4774 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3905 CTCGGG C^[CT]CG[GA]G
BalI 4403 TGGCCA TGG^CCA
BamHI 3597 GGATCC G^GATCC
BbrPI 361 CACGTG CAC^GTG
BciVI 3376 GTATCC GTATCC(6/5)
BfrI 3844 CTTAAG C^TTAAG
BlpI 3788 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3788 GCTTAGC GC^T[ACGT]AGC
BsaHI 4327 GACGCC G[GA]^CG[CT]C
BsaI 3944 GAGACC GGTCTC(1/5)
BsaWI 1110 TCCGGA [AT]^CCGG[AT]
BseAI 1110 TCCGGA T^CCGGA
BsoBI 3905 CTCGGG C^[CT]CG[GA]G
BspEI 1110 TCCGGA T^CCGGA
BspLU11I 2683 ACATGT A^CATGT
BstAPI 2434 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3788 GCTTAGC GC^T[ACGT]AGC
DraIII 361 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1836 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4403 TGGCCA [CT]^GGCC[GA]
Eam1105I 4774 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4096 AGCGCT AGC^GCT
EcoRI 4559 GAATTC G^AATTC
EcoRV 756 GATATC GAT^ATC
HgaI 4327 GACGC GACGC(5/10)
Hin1I 4327 GACGCC G[GA]^CG[CT]C
HincII 832 GTTGAC GT[CT]^[GA]AC
HindII 832 GTTGAC GT[CT]^[GA]AC
HindIII 2416 AAGCTT A^AGCTT
KpnI 3936 GGTACC GGTAC^C
MfeI 2630 CAATTG C^AATTG
MluNI 4403 TGGCCA TGG^CCA
MroI 1110 TCCGGA T^CCGGA
MscI 4403 TGGCCA TGG^CCA
MunI 2630 CAATTG C^AATTG
PmaCI 361 CACGTG CAC^GTG
PmlI 361 CACGTG CAC^GTG
PshAI 3706 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3991 GAGCTC GAGCT^C
SapI 3736 GAAGAGC GCTCTTC(1/4)
ScaI 4796 AGTACT AGT^ACT
SexAI 2090 ACCTGGT A^CC[AT]GGT
StuI 2597 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 12 enzymes) #
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AviII 1976 TGCGCA TGC^GCA
4038 TGCGCA
BsaAI 361 CACGTG [CT]AC^GT[GA]
1357 TACGTG
BspHI 2231 TCATGA T^CATGA
2511 TCATGA
BspMI 1157 GCAGGT ACCTGC(4/8)
1323 GCAGGT
DraI 1893 TTTAAA TTT^AAA
2844 TTTAAA
EcoNI 776 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1065 CCTTTTCCAGG
FauI 1796 CCCGC CCCGC(4/6)
1899 GCGGG
FspI 1976 TGCGCA TGC^GCA
4038 TGCGCA
HaeII 45 GGCGCT [GA]GCGC^[CT]
4096 AGCGCT
NcoI 2649 CCATGG C^CATGG
3511 CCATGG
PstI 1304 CTGCAG CTGCA^G
2591 CTGCAG
RcaI 2231 TCATGA T^CATGA
2511 TCATGA
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI