Restriction map for
pFN21AE3469
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccII AccIII AclI AgeI
AhdI AscI AseI AsnI AspEI
AspI AviII AvrII BbeI BbrPI
BciVI BcnI BglI BlnI BsaAI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrDI BssHII Bst1107I BstUI
BstXI BstZ17I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I EcoRI
EcoT22I FseI FspI KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 46 GGTACC G^GTACC
AccI 517 GTCTAC GT^[AC][GT]AC
AcyI 2896 GACGCC G[GA]^CG[CT]C
Aor51HI 434 AGCGCT AGC^GCT
ApaI 2364 GGGCCC GGGCC^C
Asp718I 46 GGTACC G^GTACC
BalI 1760 TGGCCA TGG^CCA
BclI 1313 TGATCA T^GATCA
BlpI 73 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 73 GCTCAGC GC^T[ACGT]AGC
BsaBI 241 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2896 GACGCC G[GA]^CG[CT]C
BsaI 3296 GGTCTC GGTCTC(1/5)
Bsp1407I 1197 TGTACA T^GTACA
BsrBI 1104 CCGCTC CCGCTC(-3/-3)
BsrFI 2654 ACCGGC [GA]^CCGG[CT]
BsrGI 1197 TGTACA T^GTACA
BstAPI 1044 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1858 TTCGAA TT^CGAA
BstEII 337 GGTGACC G^GT[ACGT]ACC
BstPI 337 GGTGACC G^GT[ACGT]ACC
CelII 73 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2654 ACCGGC [GA]^CCGG[CT]
DraI 2376 TTTAAA TTT^AAA
DrdI 3704 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 434 AGCGCT AGC^GCT
EcoO65I 337 GGTGACC G^GT[ACGT]ACC
FbaI 1313 TGATCA T^GATCA
HaeII 434 AGCGCT [GA]GCGC^[CT]
HgaI 2896 GACGC GACGC(5/10)
Hin1I 2896 GACGCC G[GA]^CG[CT]C
HpaI 154 GTTAAC GTT^AAC
KpnI 46 GGTACC GGTAC^C
MaeII 1460 ACGT A^CGT
MamI 241 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1760 TGGCCA TGG^CCA
MscI 1760 TGGCCA TGG^CCA
NcoI 1976 CCATGG C^CATGG
NspV 1858 TTCGAA TT^CGAA
PflMI 414 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 1955 ACCAGGT A^CC[AT]GGT
SfuI 1858 TTCGAA TT^CGAA
SspBI 1197 TGTACA T^GTACA
Van91I 414 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflII 51 CTTAAG C^TTAAG
127 CTTAAG
AflIII 1172 ACATGT A^C[GA][CT]GT
2153 ACATGT
AlwNI 1919 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2816 CAGCTACTG
BbvCI 1110 CCTCAGC CCTCAGC(-5/-2)
2241 GCTGAGG
BcgI 286 GCAGCATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
965 GCATCACTATCG
BfrI 51 CTTAAG C^TTAAG
127 CTTAAG
BglII 1871 AGATCT A^GATCT
4041 AGATCT
BsaWI 681 TCCGGT [AT]^CCGG[AT]
1499 TCCGGT
BsmI 2465 GAATGC GAATGC(1/-1)
3276 GAATGC
BspLU11I 1172 ACATGT A^CATGT
2153 ACATGT
BssSI 2224 CACGAG CACGAG(-5/-1)
544 CTCGTG
EcoRV 1059 GATATC GAT^ATC
1495 GATATC
FauI 504 CCCGC CCCGC(4/6)
947 CCCGC
HindIII 3060 AAGCTT A^AGCTT
3446 AAGCTT
PaeR7I 1214 CTCGAG C^TCGAG
3174 CTCGAG
PvuII 2679 CAGCTG CAG^CTG
3524 CAGCTG
ScaI 2060 AGTACT AGT^ACT
3319 AGTACT
XcmI 2534 CCAAGGACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2699 CCAGGATGAGTGTGG
XhoI 1214 CTCGAG C^TCGAG
3174 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AocI ApaLI ApoI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI