Restriction map for
pFN21AB7816
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI AlwI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BciVI BclI BfrI BglII BlnI
BsaI BseAI BsiWI BsmBI BspEI
BspHI BspMI BssHII Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I
CpoI DraI DrdI Eam1105I Eco81I
EcoRI EcoRV FbaI FspI HgaI
HincII HindII HindIII HpaI KpnI
MflI MluI MluNI MroI MscI
NcoI NdeI NotI NruI NspV
PacI PaeR7I PflFI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AcsI 1051 GAATTT [GA]^AATT[CT]
AcyI 56 GGCGCC G[GA]^CG[CT]C
AflIII 828 ACATGT A^C[GA][CT]GT
AgeI 575 ACCGGT A^CCGGT
Alw44I 373 GTGCAC G^TGCAC
Aor51HI 1757 AGCGCT AGC^GCT
ApaI 529 GGGCCC GGGCC^C
ApaLI 373 GTGCAC G^TGCAC
ApoI 1051 GAATTT [GA]^AATT[CT]
BbeI 56 GGCGCC GGCGC^C
BbrPI 1574 CACGTG CAC^GTG
BbsI 1648 GAAGAC GAAGAC(2/6)
BbvCI 890 CCTCAGC CCTCAGC(-5/-2)
BcgI 895 GCACAGTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1648 GAAGAC GAAGAC(2/6)
BsaAI 1574 CACGTG [CT]AC^GT[GA]
BsaBI 1720 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 56 GGCGCC G[GA]^CG[CT]C
BsaWI 575 ACCGGT [AT]^CCGG[AT]
BseRI 861 CTCCTC GAGGAG(10/8)
BsgI 1510 GTGCAG GTGCAG(16/14)
Bsp1407I 825 TGTACA T^GTACA
BspDI 121 ATCGAT AT^CGAT
BspLU11I 828 ACATGT A^CATGT
BsrDI 311 GCAATG GCAATG(2/0)
BsrGI 825 TGTACA T^GTACA
BssSI 370 CTCGTG CACGAG(-5/-1)
BstEII 792 GGTTACC G^GT[ACGT]ACC
BstPI 792 GGTTACC G^GT[ACGT]ACC
ClaI 121 ATCGAT AT^CGAT
DraIII 1537 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1757 AGCGCT AGC^GCT
EcoNI 649 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 792 GGTTACC G^GT[ACGT]ACC
EcoT14I 702 CCTTGG C^C[AT][AT]GG
EcoT22I 709 ATGCAT ATGCA^T
FseI 417 GGCCGGCC GGCCGG^CC
Hin1I 56 GGCGCC G[GA]^CG[CT]C
KasI 56 GGCGCC G^GCGCC
MamI 1720 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1065 CAATTG C^AATTG
MseI 1780 TTAA T^TAA
MslI 952 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1065 CAATTG C^AATTG
NaeI 418 GCCGGC GCC^GGC
NarI 56 GGCGCC GG^CGCC
NgoMIV 418 GCCGGC G^CCGGC
NsiI 709 ATGCAT ATGCA^T
NspI 828 ACATGT [GA]CATG^[CT]
PinAI 575 ACCGGT A^CCGGT
PmaCI 1574 CACGTG CAC^GTG
PmlI 1574 CACGTG CAC^GTG
PpuMI 840 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 208 GAGCTC GAGCT^C
SexAI 1538 ACCAGGT A^CC[AT]GGT
SfoI 56 GGCGCC GGC^GCC
SmaI 160 CCCGGG CCC^GGG
SmlI 379 CTCAAG C^T[CT][GA]AG
SspBI 825 TGTACA T^GTACA
StyI 702 CCTTGG C^C[AT][AT]GG
Tru9I 1780 TTAA T^TAA
XmaCI 160 CCCGGG C^CCGGG
XmaI 160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 457 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
554 CAGTGGCTG
BanI 56 GGCGCC G^G[CT][GA]CC
429 GGTGCC
BlpI 150 GCTCAGC GC^T[ACGT]AGC
1399 GCTCAGC
Bpu1102I 150 GCTCAGC GC^T[ACGT]AGC
1399 GCTCAGC
BsiEI 99 CGGCCG CG[GA][CT]^CG
416 CGGCCG
BsmAI 727 GAGAC GTCTC(1/5)
600 GTCTC
BsmI 707 GAATGC GAATGC(1/-1)
1146 GCATTC
CelII 150 GCTCAGC GC^T[ACGT]AGC
1399 GCTCAGC
DsaI 102 CCGCGG C^C[GA][CT]GG
524 CCGCGG
EaeI 99 CGGCCG [CT]^GGCC[GA]
416 CGGCCG
EagI 99 CGGCCG C^GGCCG
416 CGGCCG
EclXI 99 CGGCCG C^GGCCG
416 CGGCCG
Eco52I 99 CGGCCG C^GGCCG
416 CGGCCG
KspI 102 CCGCGG CCGC^GG
524 CCGCGG
MaeII 1575 ACGT A^CGT
1739 ACGT
NheI 1276 GCTAGC G^CTAGC
1633 GCTAGC
PflMI 47 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
86 CCATCCGCTGG
SacII 102 CCGCGG CCGC^GG
524 CCGCGG
SapI 132 GCTCTTC GCTCTTC(1/4)
210 GCTCTTC
Tsp45I 976 GTCAC ^GT[GC]AC
1368 GTCAC
Van91I 47 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
86 CCATCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp509I TspRI TthHB8I