Restriction map for
pFN21AE3972
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwI AocI ApaI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FseI FspI
HapII Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI Tth111I XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 1025 GAATTC [GA]^AATT[CT]
Alw44I 650 GTGCAC G^TGCAC
Aor51HI 1280 AGCGCT AGC^GCT
ApaLI 650 GTGCAC G^TGCAC
ApoI 1025 GAATTC [GA]^AATT[CT]
AseI 948 ATTAAT AT^TAAT
AsnI 948 ATTAAT AT^TAAT
BbsI 290 GTCTTC GAAGAC(2/6)
BclI 1010 TGATCA T^GATCA
BpuAI 290 GTCTTC GAAGAC(2/6)
BseRI 3 GAGGAG GAGGAG(10/8)
BsgI 612 CTGCAC GTGCAG(16/14)
BsmFI 8 GGGAC GGGAC(10/14)
BspMI 32 GCAGGT ACCTGC(4/8)
BsrBI 1144 GAGCGG CCGCTC(-3/-3)
DpnII 1011 GATC ^GATC
DsaI 817 CCACGG C^C[GA][CT]GG
Eco47III 1280 AGCGCT AGC^GCT
EcoRI 1025 GAATTC G^AATTC
EcoT14I 548 CCAAGG C^C[AT][AT]GG
FbaI 1010 TGATCA T^GATCA
HaeII 1280 AGCGCT [GA]GCGC^[CT]
HgaI 394 GACGC GACGC(5/10)
MaeII 11 ACGT A^CGT
MboI 1011 GATC ^GATC
NdeII 1011 GATC ^GATC
PflMI 155 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1201 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 948 ATTAAT AT^TAAT
PvuII 1063 CAGCTG CAG^CTG
SapI 1125 GAAGAGC GCTCTTC(1/4)
Sau3AI 1011 GATC ^GATC
ScaI 360 AGTACT AGT^ACT
SpeI 567 ACTAGT A^CTAGT
StuI 593 AGGCCT AGG^CCT
StyI 548 CCAAGG C^C[AT][AT]GG
TaqI 975 TCGA T^CGA
TthHB8I 975 TCGA T^CGA
Van91I 155 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 117 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 138 CCGC CCGC(-3/-1)
1146 GCGG
AflIII 400 ACATGT A^C[GA][CT]GT
473 ACATGT
AvaII 912 GGACC G^G[AT]CC
1202 GGACC
BanI 437 GGCACC G^G[CT][GA]CC
734 GGTGCC
BsmI 795 GAATGC GAATGC(1/-1)
557 GCATTC
Bsp1407I 379 TGTACA T^GTACA
457 TGTACA
BspLU11I 400 ACATGT A^CATGT
473 ACATGT
BsrGI 379 TGTACA T^GTACA
457 TGTACA
BsrI 191 ACTGG ACTGG(1/-1)
666 ACTGG
BstAPI 728 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1289 GCAGATGCTGC
CfoI 1052 GCGC GCG^C
1281 GCGC
EarI 919 GAAGAG CTCTTC(1/4)
1125 GAAGAG
EcoT22I 320 ATGCAT ATGCA^T
1106 ATGCAT
HhaI 1052 GCGC GCG^C
1281 GCGC
HinP1I 1052 GCGC G^CGC
1281 GCGC
Ksp632I 919 GAAGAG CTCTTC(1/4)
1125 GAAGAG
MspA1I 138 CCGCTG C[AC]G^C[GT]G
1063 CAGCTG
NsiI 320 ATGCAT ATGCA^T
1106 ATGCAT
NspI 400 ACATGT [GA]CATG^[CT]
473 ACATGT
PleI 1196 GACTC GAGTC(4/5)
990 GAGTC
PstI 345 CTGCAG CTGCA^G
1287 CTGCAG
SfcI 345 CTGCAG C^T[GA][CT]AG
1287 CTGCAG
SspBI 379 TGTACA T^GTACA
457 TGTACA
TfiI 366 GAATC G^A[AT]TC
1187 GAATC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AfaI AluI AlwNI AspHI BbvI
BfaI BmyI BpmI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI SmlI SspI
Tru9I TseI Tsp45I Tsp509I TspRI