Restriction map for
pFN21AE2226
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AvaI AviII
AvrII BalI BbrPI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaI BsiHKAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraII DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeI MfeI MluI MluNI
MscI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 266 TCCGGA T^CCGGA
AcyI 839 GGCGCC G[GA]^CG[CT]C
AflIII 599 ACGTGT A^C[GA][CT]GT
AlwNI 313 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 921 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 342 GGTCC G^G[AT]CC
BbeI 839 GGCGCC GGCGC^C
BbsI 1023 GTCTTC GAAGAC(2/6)
BbvCI 977 CCTCAGC CCTCAGC(-5/-2)
BcgI 4 GCATTGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1023 GTCTTC GAAGAC(2/6)
BsaBI 711 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 839 GGCGCC G[GA]^CG[CT]C
BseAI 266 TCCGGA T^CCGGA
BsgI 799 GTGCAG GTGCAG(16/14)
BsiEI 14 CGACCG CG[GA][CT]^CG
BsmBI 873 CGTCTC CGTCTC(1/5)
BsmFI 284 GGGAC GGGAC(10/14)
BspEI 266 TCCGGA T^CCGGA
BspHI 719 TCATGA T^CATGA
BsrDI 5 CATTGC GCAATG(2/0)
BsrFI 664 GCCGGC [GA]^CCGG[CT]
BssSI 658 CTCGTG CACGAG(-5/-1)
Cfr10I 664 GCCGGC [GA]^CCGG[CT]
DraIII 324 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 890 GAATTC G^AATTC
EcoT14I 388 CCTTGG C^C[AT][AT]GG
HaeII 839 GGCGCC [GA]GCGC^[CT]
Hin1I 839 GGCGCC G[GA]^CG[CT]C
HinfI 862 GAGTC G^A[ACGT]TC
KasI 839 GGCGCC G^GCGCC
MaeIII 46 GTCAC ^GT[ACGT]AC
MamI 711 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 266 TCCGGA T^CCGGA
NaeI 664 GCCGGC GCC^GGC
NarI 839 GGCGCC GG^CGCC
NgoMIV 664 GCCGGC G^CCGGC
PleI 862 GAGTC GAGTC(4/5)
PshAI 857 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 719 TCATGA T^CATGA
SfoI 839 GGCGCC GGC^GCC
SmlI 859 CTTGAG C^T[CT][GA]AG
StuI 803 AGGCCT AGG^CCT
StyI 388 CCTTGG C^C[AT][AT]GG
Tsp45I 46 GTCAC ^GT[GC]AC
TspRI 609 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 843 CCAGCTGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 921 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 471 GAATTT [GA]^AATT[CT]
890 GAATTC
AhdI 30 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
165 GACCCCGTGTC
ApoI 471 GAATTT [GA]^AATT[CT]
890 GAATTC
AspEI 30 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
165 GACCCCGTGTC
BanI 129 GGTGCC G^G[CT][GA]CC
839 GGCGCC
BglI 303 GCCCAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
400 GCCGGGGAGGC
BsaWI 266 TCCGGA [AT]^CCGG[AT]
622 ACCGGA
BseRI 820 CTCCTC GAGGAG(10/8)
97 GAGGAG
BsmAI 173 GTCTC GTCTC(1/5)
874 GTCTC
EaeI 272 TGGCCG [CT]^GGCC[GA]
662 TGGCCG
Eam1105I 30 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
165 GACCCCGTGTC
EcoNI 216 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
977 CCTCAGCCAGG
MaeII 599 ACGT A^CGT
872 ACGT
MslI 346 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
360 CATGGAGGTG
PvuII 540 CAGCTG CAG^CTG
844 CAGCTG
SexAI 125 ACCTGGT A^CC[AT]GGT
338 ACCAGGT
TaqI 13 TCGA T^CGA
449 TCGA
Tsp509I 472 AATT ^AATT
891 AATT
TthHB8I 13 TCGA T^CGA
449 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AlwI BamHI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BsiYI BslI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfcI TseI XhoII