Restriction map for
pFN21AE2199
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# NO CUT ENZYMES ( 197 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DpnII DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspA1I MunI MvnI NarI NciI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 95 CCGC CCGC(-3/-1)
AcsI 135 GAATTT [GA]^AATT[CT]
ApoI 135 GAATTT [GA]^AATT[CT]
AspHI 171 GTGCTC G[AT]GC[AT]^C
BmyI 171 GTGCTC G[AGT]GC[ACT]^C
BpmI 265 CTGGAG CTGGAG(16/14)
BsaI 6 GGTCTC GGTCTC(1/5)
BsaJI 41 CCATGG C^C[ACGT][ACGT]GG
BsiEI 92 CGACCG CG[GA][CT]^CG
BsiHKAI 171 GTGCTC G[AT]GC[AT]^C
BsiYI 228 CCCCAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 228 CCCCAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 157 GAGACG CGTCTC(1/5)
Bsp1286I 171 GTGCTC G[AGT]GC[ACT]^C
BsrFI 209 GCCGGC [GA]^CCGG[CT]
Cfr10I 209 GCCGGC [GA]^CCGG[CT]
DdeI 271 CTGAG C^T[ACGT]AG
DraII 59 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 41 CCATGG C^C[GA][CT]GG
EcoO109I 59 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 41 CCATGG C^C[AT][AT]GG
HaeIII 208 GGCC GG^CC
HapII 210 CCGG C^CGG
HinfI 126 GATTC G^A[ACGT]TC
HpaII 210 CCGG C^CGG
MaeII 254 ACGT A^CGT
MboII 191 TCTTC GAAGA(8/7)
MspI 210 CCGG C^CGG
NaeI 209 GCCGGC GCC^GGC
NcoI 41 CCATGG C^CATGG
NgoMIV 209 GCCGGC G^CCGGC
NlaIII 42 CATG CATG^
NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC
PpuMI 59 AGGTCCT [GA]G^G[AT]CC[CT]
SexAI 56 ACCAGGT A^CC[AT]GGT
StyI 41 CCATGG C^C[AT][AT]GG
TfiI 126 GATTC G^A[AT]TC
Tsp509I 136 AATT ^AATT
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 60 GGTCC G^G[AT]CC
243 GGTCC
BseRI 31 CTCCTC GAGGAG(10/8)
235 GAGGAG
BsgI 36 CTGCAC GTGCAG(16/14)
68 CTGCAC
BsmAI 157 GAGAC GTCTC(1/5)
7 GTCTC
BspMI 3 GCAGGT ACCTGC(4/8)
213 GCAGGT
BssKI 57 CCAGG ^CC[ACGT]GG
264 CCTGG
BstAPI 70 GCACCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
164 GCAGCCAGTGC
BstNI 57 CCAGG CC^[AT]GG
264 CCTGG
Cac8I 97 GCCTGC GC[ACGT]^[ACGT]GC
209 GCCGGC
Cfr13I 60 GGTCC G^G[ACGT]CC
243 GGTCC
EcoRII 57 CCAGG ^CC[AT]GG
264 CCTGG
MvaI 57 CCAGG CC^[AT]GG
264 CCTGG
Sau96I 60 GGTCC G^G[ACGT]CC
243 GGTCC
ScrFI 57 CCAGG CC^[ACGT]GG
264 CCTGG
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI BbvI BsrI Fnu4HI ItaI
MnlI MwoI TseI TspRI