Restriction map for
pFN21AE2015
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI MaeII MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 735 GTCTAC GT^[AC][GT]AC
AccII 616 CGCG CG^CG
AhdI 349 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 598 GGATC GGATC(4/5)
AocI 1166 CCTCAGG CC^T[ACGT]AGG
Asp700I 1115 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 349 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 170 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 225 GAGCTC G[GA]GC[CT]^C
BglI 1580 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 159 CTCCAG CTGGAG(16/14)
BsaBI 367 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 796 ACCGGA [AT]^CCGG[AT]
BspHI 971 TCATGA T^CATGA
BsrDI 1136 GCAATG GCAATG(2/0)
BstAPI 1127 GCAATCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 610 GGTGACC G^GT[ACGT]ACC
BstF5I 576 GGATG GGATG(2/0)
BstPI 610 GGTGACC G^GT[ACGT]ACC
BstUI 616 CGCG CG^CG
BstXI 75 CCAAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1166 CCTCAGG CC^T[ACGT]AGG
Cac8I 236 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 1761 GCGC GCG^C
DraI 760 TTTAAA TTT^AAA
DraII 585 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 349 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1166 CCTCAGG CC^T[ACGT]AGG
EcoNI 589 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 585 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 610 GGTGACC G^GT[ACGT]ACC
FauI 533 GCGGG CCCGC(4/6)
FokI 576 GGATG GGATG(9/13)
HaeIII 1006 GGCC GG^CC
HhaI 1761 GCGC GCG^C
HinP1I 1761 GCGC G^CGC
HincII 332 GTCAAC GT[CT]^[GA]AC
HindII 332 GTCAAC GT[CT]^[GA]AC
KspI 615 CCGCGG CCGC^GG
MamI 367 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 936 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 616 CGCG CG^CG
NcoI 924 CCATGG C^CATGG
NlaIV 655 GGACCC GG[ACGT]^[ACGT]CC
PflFI 170 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1156 GACTC GAGTC(4/5)
PpuMI 585 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 971 TCATGA T^CATGA
SacI 225 GAGCTC GAGCT^C
SacII 615 CCGCGG CCGC^GG
SapI 299 GAAGAGC GCTCTTC(1/4)
Tth111I 170 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 318 CCAGCCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1115 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AlwNI 665 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
677 CAGAATCTG
AspHI 225 GAGCTC G[AT]GC[AT]^C
302 GAGCAC
AvaII 586 GGTCC G^G[AT]CC
655 GGACC
BbsI 792 GAAGAC GAAGAC(2/6)
959 GAAGAC
BcnI 1182 CCGGG CC^[GC]GG
1581 CCGGG
BfaI 956 CTAG C^TAG
1145 CTAG
BmyI 225 GAGCTC G[AGT]GC[ACT]^C
302 GAGCAC
BpuAI 792 GAAGAC GAAGAC(2/6)
959 GAAGAC
BsiHKAI 225 GAGCTC G[AT]GC[AT]^C
302 GAGCAC
Bsp1286I 225 GAGCTC G[AGT]GC[ACT]^C
302 GAGCAC
BsrI 902 ACTGG ACTGG(1/-1)
161 CCAGT
BstNI 67 CCAGG CC^[AT]GG
255 CCAGG
Cfr13I 586 GGTCC G^G[ACGT]CC
655 GGACC
DdeI 887 CTAAG C^T[ACGT]AG
1167 CTCAG
EarI 299 GAAGAG CTCTTC(1/4)
581 GAAGAG
EcoRII 67 CCAGG ^CC[AT]GG
255 CCAGG
HphI 34 GGTGA GGTGA(8/7)
610 GGTGA
Ksp632I 299 GAAGAG CTCTTC(1/4)
581 GAAGAG
MaeI 956 CTAG C^TAG
1145 CTAG
MseI 761 TTAA T^TAA
938 TTAA
MvaI 67 CCAGG CC^[AT]GG
255 CCAGG
NciI 1182 CCGGG CC^[GC]GG
1581 CCGGG
NdeI 344 CATATG CA^TATG
383 CATATG
PflMI 322 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
896 CCATTGACTGG
PshAI 325 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1156 GACTCTCGTC
Sau96I 586 GGTCC G^G[ACGT]CC
655 GGACC
SfaNI 667 GATGC GCATC(5/9)
91 GCATC
Tru9I 761 TTAA T^TAA
938 TTAA
Van91I 322 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
896 CCATTGACTGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
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AciI AcsI AluI ApoI BbvI
BclI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BssKI DpnII
DsaI EcoRI EcoT14I FbaI Fnu4HI
HapII HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MwoI NdeII NlaIII Sau3AI ScrFI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I