Restriction map for
pFN21AE1610
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# NO CUT ENZYMES ( 202 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BseAI BsiEI BsiHKAI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AflII 85 CTTAAG C^TTAAG
AluI 48 AGCT AG^CT
AlwI 268 GATCC GGATC(4/5)
BanII 105 GGGCTC G[GA]GC[CT]^C
BcnI 72 CCGGG CC^[GC]GG
BfrI 85 CTTAAG C^TTAAG
BmyI 105 GGGCTC G[AGT]GC[ACT]^C
BsaWI 111 TCCGGT [AT]^CCGG[AT]
BseRI 64 GAGGAG GAGGAG(10/8)
BsgI 44 GTGCAG GTGCAG(16/14)
BsmBI 53 GAGACG CGTCTC(1/5)
Bsp1286I 105 GGGCTC G[AGT]GC[ACT]^C
BsrDI 218 GCAATG GCAATG(2/0)
BstF5I 167 CATCC GGATG(2/0)
Cac8I 135 GCAAGC GC[ACGT]^[ACGT]GC
DdeI 203 CTCAG C^T[ACGT]AG
DpnII 268 GATC ^GATC
EcoNI 225 CCTATTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 167 CATCC GGATG(9/13)
HgaI 55 GACGC GACGC(5/10)
MboI 268 GATC ^GATC
MboII 13 TCTTC GAAGA(8/7)
MspA1I 47 CAGCTG C[AC]G^C[GT]G
NciI 72 CCGGG CC^[GC]GG
NdeII 268 GATC ^GATC
NlaIV 74 GGGTCC GG[ACGT]^[ACGT]CC
PvuII 47 CAGCTG CAG^CTG
Sau3AI 268 GATC ^GATC
SfcI 250 CTACAG C^T[GA][CT]AG
TspRI 276 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 170 CCGC CCGC(-3/-1)
99 GCGG
BbvI 46 GCAGC GCAGC(8/12)
117 GCTGC
BpmI 176 CTCCAG CTGGAG(16/14)
50 CTGGAG
BsiYI 72 CCGGGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
225 CCTATTTAAGG
BslI 72 CCGGGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
225 CCTATTTAAGG
BsmAI 53 GAGAC GTCTC(1/5)
201 GTCTC
BstNI 78 CCAGG CC^[AT]GG
298 CCAGG
EcoRII 78 CCAGG ^CC[AT]GG
298 CCAGG
Fnu4HI 46 GCAGC GC^[ACGT]GC
117 GCTGC
HapII 72 CCGG C^CGG
112 CCGG
HpaII 72 CCGG C^CGG
112 CCGG
HphI 61 GGTGA GGTGA(8/7)
16 TCACC
ItaI 46 GCAGC GC^[ACGT]GC
117 GCTGC
MseI 86 TTAA T^TAA
230 TTAA
MspI 72 CCGG C^CGG
112 CCGG
MvaI 78 CCAGG CC^[AT]GG
298 CCAGG
MwoI 49 GCTGGAGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
172 GCACCTCCAGC
SmlI 85 CTTAAG C^T[CT][GA]AG
324 CTTGAG
Tru9I 86 TTAA T^TAA
230 TTAA
TseI 46 GCAGC G^C[AT]GC
117 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AvaII BssKI Cfr13I MnlI Sau96I
ScrFI