Restriction map for
pFN21AE5286
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HpaI HphI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Van91I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 213 CGCG CG^CG
AfaI 150 GTAC GT^AC
Alw44I 301 GTGCAC G^TGCAC
AlwI 195 GATCC GGATC(4/5)
AocI 117 CCTGAGG CC^T[ACGT]AGG
Aor51HI 162 AGCGCT AGC^GCT
ApaLI 301 GTGCAC G^TGCAC
AspI 634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 428 TGGCCA TGG^CCA
BlpI 339 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 339 GCTGAGC GC^T[ACGT]AGC
BsaI 674 GGTCTC GGTCTC(1/5)
BseRI 592 GAGGAG GAGGAG(10/8)
BsiEI 78 CGACCG CG[GA][CT]^CG
BsrBI 556 GAGCGG CCGCTC(-3/-3)
BsrFI 22 GCCGGC [GA]^CCGG[CT]
BstUI 213 CGCG CG^CG
BstYI 194 AGATCC [GA]^GATC[CT]
Bsu36I 117 CCTGAGG CC^T[ACGT]AGG
CelII 339 GCTGAGC GC^T[ACGT]AGC
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
DpnII 195 GATC ^GATC
Eco47III 162 AGCGCT AGC^GCT
Eco81I 117 CCTGAGG CC^T[ACGT]AGG
EcoNI 588 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 54 CCAAGG C^C[AT][AT]GG
HaeII 162 AGCGCT [GA]GCGC^[CT]
HgaI 211 GACGC GACGC(5/10)
HindIII 582 AAGCTT A^AGCTT
MboI 195 GATC ^GATC
MflI 194 AGATCC [GA]^GATC[CT]
MluNI 428 TGGCCA TGG^CCA
MscI 428 TGGCCA TGG^CCA
MvnI 213 CGCG CG^CG
NaeI 22 GCCGGC GCC^GGC
NdeII 195 GATC ^GATC
NgoMIV 22 GCCGGC G^CCGGC
PaeR7I 613 CTCGAG C^TCGAG
PflFI 634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 526 CTGCAG CTGCA^G
PvuII 257 CAGCTG CAG^CTG
RsaI 150 GTAC GT^AC
SacI 342 GAGCTC GAGCT^C
Sau3AI 195 GATC ^GATC
SfcI 526 CTGCAG C^T[GA][CT]AG
SmlI 613 CTCGAG C^T[CT][GA]AG
StyI 54 CCAAGG C^C[AT][AT]GG
TspRI 432 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 613 CTCGAG C^TCGAG
XhoII 194 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwNI 176 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
529 CAGCCGCTG
ApaI 413 GGGCCC GGGCC^C
476 GGGCCC
AvaI 499 CCCGAG C^[CT]CG[GA]G
613 CTCGAG
BanI 25 GGCACC G^G[CT][GA]CC
574 GGCACC
BbsI 655 GAAGAC GAAGAC(2/6)
640 GTCTTC
BcnI 81 CCGGG CC^[GC]GG
318 CCGGG
BfaI 400 CTAG C^TAG
631 CTAG
BpuAI 655 GAAGAC GAAGAC(2/6)
640 GTCTTC
BsgI 248 CTGCAC GTGCAG(16/14)
381 CTGCAC
BsmFI 241 GGGAC GGGAC(10/14)
321 GGGAC
BsoBI 499 CCCGAG C^[CT]CG[GA]G
613 CTCGAG
BspMI 137 ACCTGC ACCTGC(4/8)
659 ACCTGC
BstF5I 105 CATCC GGATG(2/0)
84 GGATG
DsaI 144 CCGTGG C^C[GA][CT]GG
488 CCGTGG
EaeI 63 CGGCCA [CT]^GGCC[GA]
428 TGGCCA
FokI 105 CATCC GGATG(9/13)
84 GGATG
HinfI 388 GACTC G^A[ACGT]TC
634 GACTC
MaeI 400 CTAG C^TAG
631 CTAG
MboII 655 GAAGA GAAGA(8/7)
641 TCTTC
MspA1I 257 CAGCTG C[AC]G^C[GT]G
532 CCGCTG
NciI 81 CCGGG CC^[GC]GG
318 CCGGG
NlaIII 12 CATG CATG^
33 CATG
PleI 388 GACTC GAGTC(4/5)
634 GACTC
PpuMI 241 GGGACCC [GA]G^G[AT]CC[CT]
321 GGGACCC
StuI 92 AGGCCT AGG^CCT
230 AGGCCT
TaqI 77 TCGA T^CGA
614 TCGA
Tsp509I 49 AATT ^AATT
112 AATT
TthHB8I 77 TCGA T^CGA
614 TCGA
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MslI MspI
MvaI MwoI NlaIV Sau96I ScrFI
TseI