Restriction map for
pFN21AE2250
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# NO CUT ENZYMES ( 201 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DraII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tsp45I Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AciI 474 CCGC CCGC(-3/-1)
BanI 236 GGCACC G^G[CT][GA]CC
BbsI 32 GAAGAC GAAGAC(2/6)
BpmI 134 CTCCAG CTGGAG(16/14)
BpuAI 32 GAAGAC GAAGAC(2/6)
BsaJI 289 CCTGGG C^C[ACGT][ACGT]GG
BsrI 61 CCAGT ACTGG(1/-1)
BssKI 289 CCTGG ^CC[ACGT]GG
BstNI 289 CCTGG CC^[AT]GG
Cfr13I 130 GGCCC G^G[ACGT]CC
DpnII 100 GATC ^GATC
DraI 457 TTTAAA TTT^AAA
DraIII 343 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRII 289 CCTGG ^CC[AT]GG
FauI 473 CCCGC CCCGC(4/6)
HaeIII 130 GGCC GG^CC
HinfI 400 GACTC G^A[ACGT]TC
MboI 100 GATC ^GATC
MvaI 289 CCTGG CC^[AT]GG
MwoI 30 GCGAAGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 100 GATC ^GATC
PflMI 342 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 400 GACTC GAGTC(4/5)
PvuII 37 CAGCTG CAG^CTG
Sau3AI 100 GATC ^GATC
Sau96I 130 GGCCC G^G[ACGT]CC
ScrFI 289 CCTGG CC^[ACGT]GG
SfaNI 418 GATGC GCATC(5/9)
Van91I 342 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 319 CCAGAGGAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 217 AAATTC [GA]^AATT[CT]
454 GAATTT
AluI 8 AGCT AG^CT
38 AGCT
ApoI 217 AAATTC [GA]^AATT[CT]
454 GAATTT
BbvI 9 GCTGC GCAGC(8/12)
421 GCTGC
BsiYI 61 CCAGTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
342 CCACACTGTGG
BslI 61 CCAGTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
342 CCACACTGTGG
BsmAI 398 GAGAC GTCTC(1/5)
496 GAGAC
BspHI 282 TCATGA T^CATGA
359 TCATGA
BspMI 405 ACCTGC ACCTGC(4/8)
79 GCAGGT
Fnu4HI 9 GCTGC GC^[ACGT]GC
421 GCTGC
HphI 259 GGTGA GGTGA(8/7)
403 TCACC
ItaI 9 GCTGC GC^[ACGT]GC
421 GCTGC
MslI 238 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
372 CATTTCTATG
MspA1I 37 CAGCTG C[AC]G^C[GT]G
474 CCGCTG
NlaIII 283 CATG CATG^
360 CATG
NlaIV 236 GGCACC GG[ACGT]^[ACGT]CC
332 GGCTCC
PstI 10 CTGCAG CTGCA^G
77 CTGCAG
RcaI 282 TCATGA T^CATGA
359 TCATGA
SfcI 10 CTGCAG C^T[GA][CT]AG
77 CTGCAG
TseI 9 GCTGC G^C[AT]GC
421 GCTGC
Tsp509I 218 AATT ^AATT
455 AATT
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
Cac8I MboII MnlI MseI Tru9I
TspRI