Restriction map for
pFN21AB9768
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# NO CUT ENZYMES ( 83 enzymes) #
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AccI AccIII AclI AcsI AflII
AhdI AocI Aor51HI ApoI AseI
AsnI Asp700I AspEI AvrII BamHI
BcgI BciVI BfaI BfrI BlnI
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
HindIII MaeI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SexAI
SfuI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tsp509I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 715 GACGTC GACGT^C
Acc65I 746 GGTACC G^GTACC
AflIII 1586 ACGTGT A^C[GA][CT]GT
AgeI 2918 ACCGGT A^CCGGT
AscI 2644 GGCGCGCC GG^CGCGCC
Asp718I 746 GGTACC G^GTACC
AspI 1464 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1628 TGGCCA TGG^CCA
BbsI 718 GTCTTC GAAGAC(2/6)
BbvCI 444 GCTGAGG CCTCAGC(-5/-2)
BclI 704 TGATCA T^GATCA
BglII 1142 AGATCT A^GATCT
BlpI 1035 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1035 GCTGAGC GC^T[ACGT]AGC
BpuAI 718 GTCTTC GAAGAC(2/6)
BsaWI 2918 ACCGGT [AT]^CCGG[AT]
CelII 1035 GCTGAGC GC^T[ACGT]AGC
DrdI 2860 GACAAGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 704 TGATCA T^GATCA
HpaI 148 GTTAAC GTT^AAC
KpnI 746 GGTACC GGTAC^C
MluNI 1628 TGGCCA TGG^CCA
MscI 1628 TGGCCA TGG^CCA
MseI 149 TTAA T^TAA
NcoI 354 CCATGG C^CATGG
NspI 929 ACATGC [GA]CATG^[CT]
PaeR7I 2485 CTCGAG C^TCGAG
PflFI 1464 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2918 ACCGGT A^CCGGT
PvuII 1053 CAGCTG CAG^CTG
SbfI 765 CCTGCAGG CCTGCA^GG
ScaI 2510 AGTACT AGT^ACT
SfiI 672 GGCCGGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 765 CCTGCAGG CCTGCA^GG
Tru9I 149 TTAA T^TAA
Tth111I 1464 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2485 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AviII 932 TGCGCA TGC^GCA
2213 TGCGCA
BbrPI 627 CACGTG CAC^GTG
3010 CACGTG
BsaI 850 GAGACC GGTCTC(1/5)
2086 GAGACC
BsrI 1281 ACTGG ACTGG(1/-1)
885 CCAGT
BstEII 525 GGTCACC G^GT[ACGT]ACC
1101 GGTCACC
BstPI 525 GGTCACC G^GT[ACGT]ACC
1101 GGTCACC
DraIII 598 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2046 CACGCTGTG
EarI 1705 CTCTTC CTCTTC(1/4)
238 GAAGAG
EcoNI 465 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1296 CCTGGCCGAGG
EcoO65I 525 GGTCACC G^GT[ACGT]ACC
1101 GGTCACC
FseI 2174 GGCCGGCC GGCCGG^CC
2655 GGCCGGCC
FspI 932 TGCGCA TGC^GCA
2213 TGCGCA
HincII 148 GTTAAC GT[CT]^[GA]AC
1765 GTCAAC
HindII 148 GTTAAC GT[CT]^[GA]AC
1765 GTCAAC
Ksp632I 1705 CTCTTC CTCTTC(1/4)
238 GAAGAG
MaeIII 526 GTCAC ^GT[ACGT]AC
1102 GTCAC
PmaCI 627 CACGTG CAC^GTG
3010 CACGTG
PmlI 627 CACGTG CAC^GTG
3010 CACGTG
PstI 766 CTGCAG CTGCA^G
1660 CTGCAG
SapI 238 GAAGAGC GCTCTTC(1/4)
1704 GCTCTTC
SfaNI 740 GCATC GCATC(5/9)
1382 GCATC
SmlI 1015 CTCAAG C^T[CT][GA]AG
2485 CTCGAG
Tsp45I 526 GTCAC ^GT[GC]AC
1102 GTCAC
TspRI 701 CAGTG CA[GC]TG[ACGT][ACGT]^
886 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI RsaI SacII Sau3AI
Sau96I ScrFI SfcI SfoI SgrAI
SmaI StuI StyI TaqI TseI
TthHB8I XhoII XmaCI XmaI