Restriction map for
pFN21AE3200
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# NO CUT ENZYMES ( 51 enzymes) #
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AclI AflII AhdI AseI AsnI
AspEI AvrII BbrPI BciVI BfrI
BlnI BsiWI BspDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
Eam1105I EcoO65I EcoRV EcoT22I HpaI
KspI MluI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 979 GACGTC GACGT^C
Alw44I 2074 GTGCAC G^TGCAC
AocI 4143 CCTAAGG CC^T[ACGT]AGG
ApaLI 2074 GTGCAC G^TGCAC
AscI 5585 GGCGCGCC GG^CGCGCC
AspI 25 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5973 TGGCCA TGG^CCA
BbvCI 834 GCTGAGG CCTCAGC(-5/-2)
BglII 5231 AGATCT A^GATCT
BsaAI 4533 TACGTG [CT]AC^GT[GA]
BsaBI 2962 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 2411 ACATGT A^CATGT
Bsu36I 4143 CCTAAGG CC^T[ACGT]AGG
Eco81I 4143 CCTAAGG CC^T[ACGT]AGG
EcoRI 4149 GAATTC G^AATTC
FseI 5950 GGCCGGCC GGCCGG^CC
MamI 2962 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4211 CAATTG C^AATTG
MluNI 5973 TGGCCA TGG^CCA
MscI 5973 TGGCCA TGG^CCA
MunI 4211 CAATTG C^AATTG
NdeI 301 CATATG CA^TATG
NheI 903 GCTAGC G^CTAGC
PflFI 25 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 2638 GTCGAC G^TCGAC
SexAI 3790 ACCTGGT A^CC[AT]GGT
SphI 3903 GCATGC GCATG^C
SspI 3526 AATATT AAT^ATT
Tth111I 25 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2269 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 35 enzymes) #
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Acc65I 2423 GGTACC G^GTACC
4196 GGTACC
AflIII 1688 ACGTGT A^C[GA][CT]GT
2411 ACATGT
AgeI 218 ACCGGT A^CCGGT
734 ACCGGT
Asp700I 2950 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4395 GAAGGCCTTC
Asp718I 2423 GGTACC G^GTACC
4196 GGTACC
AviII 4911 TGCGCA TGC^GCA
5285 TGCGCA
BbeI 3068 GGCGCC GGCGC^C
4436 GGCGCC
BcgI 3555 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3750 CGACCAGAGTGC
BlpI 795 GCTGAGC GC^T[ACGT]AGC
5826 GCTGAGC
Bpu1102I 795 GCTGAGC GC^T[ACGT]AGC
5826 GCTGAGC
Bsp1407I 406 TGTACA T^GTACA
2867 TGTACA
BspHI 4049 TCATGA T^CATGA
6029 TCATGA
BsrDI 3831 CATTGC GCAATG(2/0)
3839 GCAATG
BsrGI 406 TGTACA T^GTACA
2867 TGTACA
BssHII 5401 GCGCGC G^CGCGC
5586 GCGCGC
BssSI 6295 CACGAG CACGAG(-5/-1)
2649 CTCGTG
BstXI 64 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5501 CCAGACCCCTGG
CelII 795 GCTGAGC GC^T[ACGT]AGC
5826 GCTGAGC
DraI 1015 TTTAAA TTT^AAA
4099 TTTAAA
DraIII 1953 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5443 CACTCCGTG
DrdI 1045 GACGAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3471 GACATTTCTGTC
FspI 4911 TGCGCA TGC^GCA
5285 TGCGCA
KasI 3068 GGCGCC G^GCGCC
4436 GGCGCC
KpnI 2423 GGTACC GGTAC^C
4196 GGTACC
NarI 3068 GGCGCC GG^CGCC
4436 GGCGCC
NspI 2411 ACATGT [GA]CATG^[CT]
3903 GCATGC
PinAI 218 ACCGGT A^CCGGT
734 ACCGGT
RcaI 4049 TCATGA T^CATGA
6029 TCATGA
SfoI 3068 GGCGCC GGC^GCC
4436 GGCGCC
SmaI 162 CCCGGG CCC^GGG
5570 CCCGGG
SmlI 997 CTCAAG C^T[CT][GA]AG
3379 CTCAAG
SspBI 406 TGTACA T^GTACA
2867 TGTACA
XmaCI 162 CCCGGG C^CCGGG
5570 CCCGGG
XmaI 162 CCCGGG C^CCGGG
5570 CCCGGG
XmnI 2950 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4395 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
Aor51HI ApaI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII