Restriction map for
pFN21AE3335
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# NO CUT ENZYMES ( 90 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI ApaI ApoI AscI
Asp700I Asp718I AspI AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BstBI
Cfr10I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRI EcoRV
FbaI FseI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SfiI SfuI
SgrAI SpeI SphI Sse8387I StuI
SwaI Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 2521 GACGTC GACGT^C
Alw44I 2044 GTGCAC G^TGCAC
ApaLI 2044 GTGCAC G^TGCAC
AseI 444 ATTAAT AT^TAAT
AsnI 444 ATTAAT AT^TAAT
AviII 2590 TGCGCA TGC^GCA
BalI 117 TGGCCA TGG^CCA
BbeI 2205 GGCGCC GGCGC^C
BbsI 511 GTCTTC GAAGAC(2/6)
BbvCI 1263 GCTGAGG CCTCAGC(-5/-2)
BfaI 1451 CTAG C^TAG
BglII 2643 AGATCT A^GATCT
BlpI 1669 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1669 GCTGAGC GC^T[ACGT]AGC
BpuAI 511 GTCTTC GAAGAC(2/6)
BsaWI 2657 TCCGGT [AT]^CCGG[AT]
Bsp1407I 413 TGTACA T^GTACA
BsrBI 1885 GAGCGG CCGCTC(-3/-3)
BsrDI 1908 CATTGC GCAATG(2/0)
BsrGI 413 TGTACA T^GTACA
Bst1107I 633 GTATAC GTA^TAC
BstEII 639 GGTCACC G^GT[ACGT]ACC
BstPI 639 GGTCACC G^GT[ACGT]ACC
BstYI 2643 AGATCT [GA]^GATC[CT]
BstZ17I 633 GTATAC GTA^TAC
CelII 1669 GCTGAGC GC^T[ACGT]AGC
DraIII 2038 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 639 GGTCACC G^GT[ACGT]ACC
EcoT22I 646 ATGCAT ATGCA^T
FspI 2590 TGCGCA TGC^GCA
KasI 2205 GGCGCC G^GCGCC
KspI 1725 CCGCGG CCGC^GG
MaeI 1451 CTAG C^TAG
MflI 2643 AGATCT [GA]^GATC[CT]
MluNI 117 TGGCCA TGG^CCA
MscI 117 TGGCCA TGG^CCA
MseI 445 TTAA T^TAA
NarI 2205 GGCGCC GG^CGCC
NcoI 710 CCATGG C^CATGG
NheI 1450 GCTAGC G^CTAGC
NsiI 646 ATGCAT ATGCA^T
PaeR7I 890 CTCGAG C^TCGAG
PshBI 444 ATTAAT AT^TAAT
SacII 1725 CCGCGG CCGC^GG
ScaI 2783 AGTACT AGT^ACT
SexAI 2411 ACCTGGT A^CC[AT]GGT
SfoI 2205 GGCGCC GGC^GCC
SmlI 890 CTCGAG C^T[CT][GA]AG
SnaBI 1945 TACGTA TAC^GTA
SspBI 413 TGTACA T^GTACA
SspI 2068 AATATT AAT^ATT
TfiI 1482 GATTC G^A[AT]TC
Tru9I 445 TTAA T^TAA
XhoI 890 CTCGAG C^TCGAG
XhoII 2643 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 633 GTATAC GT^[AC][GT]AC
838 GTCTAC
AhdI 222 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
553 GACCCTGAGTC
AocI 944 CCTCAGG CC^T[ACGT]AGG
1184 CCTCAGG
Aor51HI 89 AGCGCT AGC^GCT
2248 AGCGCT
AspEI 222 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
553 GACCCTGAGTC
BsaAI 1945 TACGTA [CT]AC^GT[GA]
2287 TACGTG
BstAPI 1552 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2220 GCAGCTCCTGC
Bsu36I 944 CCTCAGG CC^T[ACGT]AGG
1184 CCTCAGG
Eam1105I 222 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
553 GACCCTGAGTC
Eco47III 89 AGCGCT AGC^GCT
2248 AGCGCT
Eco81I 944 CCTCAGG CC^T[ACGT]AGG
1184 CCTCAGG
EcoNI 556 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
940 CCTGCCTCAGG
SacI 1918 GAGCTC GAGCT^C
2728 GAGCTC
SmaI 953 CCCGGG CCC^GGG
2405 CCCGGG
XmaCI 953 CCCGGG C^CCGGG
2405 CCCGGG
XmaI 953 CCCGGG C^CCGGG
2405 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI