Restriction map for
pFN21AE9429
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI HgaI HindIII KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcsI 322 GAATTC [GA]^AATT[CT]
AcyI 762 GGCGCC G[GA]^CG[CT]C
AocI 289 CCTCAGG CC^T[ACGT]AGG
ApaI 722 GGGCCC GGGCC^C
ApoI 322 GAATTC [GA]^AATT[CT]
AspHI 4 GAGCAC G[AT]GC[AT]^C
BamHI 554 GGATCC G^GATCC
BanII 722 GGGCCC G[GA]GC[CT]^C
BbeI 762 GGCGCC GGCGC^C
BciVI 1051 GTATCC GTATCC(6/5)
BclI 143 TGATCA T^GATCA
BfaI 817 CTAG C^TAG
BsaHI 762 GGCGCC G[GA]^CG[CT]C
BsaI 414 GGTCTC GGTCTC(1/5)
BsiHKAI 4 GAGCAC G[AT]GC[AT]^C
BsmBI 649 CGTCTC CGTCTC(1/5)
BspHI 737 TCATGA T^CATGA
BspMI 463 ACCTGC ACCTGC(4/8)
BsrBI 397 CCGCTC CCGCTC(-3/-3)
BsrDI 804 CATTGC GCAATG(2/0)
BsrFI 984 GCCGGC [GA]^CCGG[CT]
BstAPI 202 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 750 CCACCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 289 CCTCAGG CC^T[ACGT]AGG
CfoI 763 GCGC GCG^C
Cfr10I 984 GCCGGC [GA]^CCGG[CT]
DrdI 217 GACATCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 636 GAAGAG CTCTTC(1/4)
Eco81I 289 CCTCAGG CC^T[ACGT]AGG
EcoRI 322 GAATTC G^AATTC
EcoT14I 881 CCATGG C^C[AT][AT]GG
FbaI 143 TGATCA T^GATCA
HaeII 762 GGCGCC [GA]GCGC^[CT]
HhaI 763 GCGC GCG^C
Hin1I 762 GGCGCC G[GA]^CG[CT]C
HinP1I 763 GCGC G^CGC
HincII 363 GTTAAC GT[CT]^[GA]AC
HindII 363 GTTAAC GT[CT]^[GA]AC
HinfI 741 GATTC G^A[ACGT]TC
HpaI 363 GTTAAC GTT^AAC
KasI 762 GGCGCC G^GCGCC
Ksp632I 636 GAAGAG CTCTTC(1/4)
MaeI 817 CTAG C^TAG
MaeII 542 ACGT A^CGT
MseI 364 TTAA T^TAA
NaeI 984 GCCGGC GCC^GGC
NarI 762 GGCGCC GG^CGCC
NcoI 881 CCATGG C^CATGG
NdeI 345 CATATG CA^TATG
NgoMIV 984 GCCGGC G^CCGGC
NheI 816 GCTAGC G^CTAGC
PpuMI 293 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 173 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 157 CAGCTG CAG^CTG
RcaI 737 TCATGA T^CATGA
SapI 636 GAAGAGC GCTCTTC(1/4)
SexAI 575 ACCTGGT A^CC[AT]GGT
SfcI 96 CTACAG C^T[GA][CT]AG
SfoI 762 GGCGCC GGC^GCC
StyI 881 CCATGG C^C[AT][AT]GG
TfiI 741 GATTC G^A[AT]TC
Tru9I 364 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 91 GTCTAC GT^[AC][GT]AC
571 GTCTAC
BanI 579 GGTGCC G^G[CT][GA]CC
762 GGCGCC
BcnI 55 CCCGG CC^[GC]GG
434 CCCGG
BsmAI 415 GTCTC GTCTC(1/5)
650 GTCTC
BsmFI 51 GTCCC GGGAC(10/14)
295 GTCCC
Bsp1407I 620 TGTACA T^GTACA
930 TGTACA
BsrGI 620 TGTACA T^GTACA
930 TGTACA
BsrI 491 ACTGG ACTGG(1/-1)
1020 CCAGT
BstNI 576 CCTGG CC^[AT]GG
974 CCTGG
BstYI 554 GGATCC [GA]^GATC[CT]
977 GGATCT
DdeI 290 CTCAG C^T[ACGT]AG
628 CTCAG
DraII 293 AGGTCCC [GA]G^G[ACGT]CC[CT]
722 GGGCCCC
EcoO109I 293 AGGTCCC [GA]G^G[ACGT]CC[CT]
722 GGGCCCC
EcoRII 576 CCTGG ^CC[AT]GG
974 CCTGG
MflI 554 GGATCC [GA]^GATC[CT]
977 GGATCT
MspA1I 157 CAGCTG C[AC]G^C[GT]G
777 CCGCTG
MvaI 576 CCTGG CC^[AT]GG
974 CCTGG
NciI 55 CCCGG CC^[GC]GG
434 CCCGG
NspI 278 ACATGC [GA]CATG^[CT]
812 ACATGC
SmlI 400 CTCAAG C^T[CT][GA]AG
898 CTCAAG
SspBI 620 TGTACA T^GTACA
930 TGTACA
Tsp509I 323 AATT ^AATT
643 AATT
XhoII 554 GGATCC [GA]^GATC[CT]
977 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BmyI BpuAI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
Cac8I Cfr13I DpnII DsaI FauI
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MboI MboII MnlI
MslI MspI MwoI NdeII NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI Van91I