Restriction map for
pFN21AE1668
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsaI
RsrII SacII SalI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 349 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 435 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 316 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 279 GGATCC G^GATCC
BanI 242 GGTGCC G^G[CT][GA]CC
BcnI 267 CCCGG CC^[GC]GG
BsgI 421 GTGCAG GTGCAG(16/14)
BsmAI 166 GAGAC GTCTC(1/5)
BsmI 50 GAATGC GAATGC(1/-1)
BspHI 323 TCATGA T^CATGA
BsrI 489 CCAGT ACTGG(1/-1)
BstAPI 348 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 270 GGATG GGATG(2/0)
Cac8I 423 GCAGGC GC[ACGT]^[ACGT]GC
DsaI 505 CCACGG C^C[GA][CT]GG
EarI 472 CTCTTC CTCTTC(1/4)
FauI 283 CCCGC CCCGC(4/6)
FokI 270 GGATG GGATG(9/13)
HapII 268 CCGG C^CGG
HinfI 97 GAATC G^A[ACGT]TC
HpaII 268 CCGG C^CGG
Ksp632I 472 CTCTTC CTCTTC(1/4)
MaeII 105 ACGT A^CGT
MaeIII 314 GTGAC ^GT[ACGT]AC
MspA1I 349 CAGCGG C[AC]G^C[GT]G
MspI 268 CCGG C^CGG
NciI 267 CCCGG CC^[GC]GG
PflFI 316 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 323 TCATGA T^CATGA
SapI 471 GCTCTTC GCTCTTC(1/4)
SexAI 361 ACCTGGT A^CC[AT]GGT
TaqI 121 TCGA T^CGA
TfiI 97 GAATC G^A[AT]TC
Tsp45I 314 GTGAC ^GT[GC]AC
Tth111I 316 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 121 TCGA T^CGA
XmnI 435 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 15 GGTCC G^G[AT]CC
411 GGTCC
BpmI 136 CTGGAG CTGGAG(16/14)
163 CTGGAG
BsmFI 512 GGGAC GGGAC(10/14)
265 GTCCC
BstYI 256 AGATCC [GA]^GATC[CT]
279 GGATCC
DpnII 257 GATC ^GATC
280 GATC
DraII 14 AGGTCCT [GA]G^G[ACGT]CC[CT]
425 AGGCCCT
EcoO109I 14 AGGTCCT [GA]G^G[ACGT]CC[CT]
425 AGGCCCT
HphI 114 GGTGA GGTGA(8/7)
387 GGTGA
MboI 257 GATC ^GATC
280 GATC
MboII 33 GAAGA GAAGA(8/7)
473 TCTTC
MflI 256 AGATCC [GA]^GATC[CT]
279 GGATCC
NdeII 257 GATC ^GATC
280 GATC
PstI 346 CTGCAG CTGCA^G
355 CTGCAG
SacI 202 GAGCTC GAGCT^C
469 GAGCTC
Sau3AI 257 GATC ^GATC
280 GATC
SfcI 346 CTGCAG C^T[GA][CT]AG
355 CTGCAG
TspRI 170 CAGTG CA[GC]TG[ACGT][ACGT]^
419 CAGTG
XhoII 256 AGATCC [GA]^GATC[CT]
279 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
Cfr13I EcoRII Fnu4HI HaeIII ItaI
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI TseI