Restriction map for
pFN21AE2208
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CpoI DdeI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 577 GAATTT [GA]^AATT[CT]
AcyI 108 GACGCC G[GA]^CG[CT]C
Alw44I 121 GTGCAC G^TGCAC
ApaLI 121 GTGCAC G^TGCAC
ApoI 577 GAATTT [GA]^AATT[CT]
BalI 762 TGGCCA TGG^CCA
BamHI 254 GGATCC G^GATCC
BanII 418 GGGCTC G[GA]GC[CT]^C
BciVI 15 GTATCC GTATCC(6/5)
BglI 68 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 683 AGATCT A^GATCT
BsaHI 108 GACGCC G[GA]^CG[CT]C
BsmAI 645 GAGAC GTCTC(1/5)
BspDI 403 ATCGAT AT^CGAT
BspMI 83 ACCTGC ACCTGC(4/8)
BsrFI 735 GCCGGC [GA]^CCGG[CT]
BsrI 758 CCAGT ACTGG(1/-1)
CfoI 87 GCGC GCG^C
Cfr10I 735 GCCGGC [GA]^CCGG[CT]
ClaI 403 ATCGAT AT^CGAT
EarI 750 GAAGAG CTCTTC(1/4)
EcoNI 93 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 108 GACGC GACGC(5/10)
HhaI 87 GCGC GCG^C
Hin1I 108 GACGCC G[GA]^CG[CT]C
HinP1I 87 GCGC G^CGC
HinfI 220 GAATC G^A[ACGT]TC
Ksp632I 750 GAAGAG CTCTTC(1/4)
MaeII 191 ACGT A^CGT
MluNI 762 TGGCCA TGG^CCA
MscI 762 TGGCCA TGG^CCA
MslI 114 CACTCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 735 GCCGGC GCC^GGC
NgoMIV 735 GCCGGC G^CCGGC
SmlI 436 CTCAAG C^T[CT][GA]AG
StuI 130 AGGCCT AGG^CCT
TfiI 220 GAATC G^A[AT]TC
Tsp509I 578 AATT ^AATT
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 570 GTAC GT^AC
670 GTAC
AspHI 121 GTGCAC G[AT]GC[AT]^C
459 GTGCTC
AvaI 532 CCCGAG C^[CT]CG[GA]G
541 CCCGAG
BcnI 73 CCCGG CC^[GC]GG
509 CCCGG
BpmI 94 CTGGAG CTGGAG(16/14)
622 CTGGAG
BsgI 526 CTGCAC GTGCAG(16/14)
745 GTGCAG
BsiHKAI 121 GTGCAC G[AT]GC[AT]^C
459 GTGCTC
BsoBI 532 CCCGAG C^[CT]CG[GA]G
541 CCCGAG
BsrBI 103 GAGCGG CCGCTC(-3/-3)
544 GAGCGG
EaeI 75 CGGCCA [CT]^GGCC[GA]
762 TGGCCA
MaeIII 373 GTGAC ^GT[ACGT]AC
379 GTGAC
MboII 303 GAAGA GAAGA(8/7)
750 GAAGA
NciI 73 CCCGG CC^[GC]GG
509 CCCGG
NlaIII 136 CATG CATG^
384 CATG
PstI 40 CTGCAG CTGCA^G
712 CTGCAG
RsaI 570 GTAC GT^AC
670 GTAC
SfaNI 492 GATGC GCATC(5/9)
56 GCATC
TaqI 404 TCGA T^CGA
422 TCGA
Tsp45I 373 GTGAC ^GT[GC]AC
379 GTGAC
TthHB8I 404 TCGA T^CGA
422 TCGA
XcmI 383 CCATGCGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
464 CCAAGTTTGGGATGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII ItaI MboI
MflI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIV PpuMI PvuII
Sau3AI Sau96I ScrFI SfcI TseI
TspRI XhoII