Restriction map for
pFN21AE5960
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MfeI MluI MroI
MseI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
AfaI 566 GTAC GT^AC
ApoI 31 GAATTT [GA]^AATT[CT]
AviII 915 TGCGCA TGC^GCA
BalI 371 TGGCCA TGG^CCA
BbvI 339 GCTGC GCAGC(8/12)
BfaI 697 CTAG C^TAG
BglII 299 AGATCT A^GATCT
BpmI 583 CTGGAG CTGGAG(16/14)
BsmAI 897 GAGAC GTCTC(1/5)
BsmFI 45 GGGAC GGGAC(10/14)
BspHI 404 TCATGA T^CATGA
BsrDI 889 GCAATG GCAATG(2/0)
BstXI 312 CCATGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 299 AGATCT [GA]^GATC[CT]
Cac8I 109 GCGAGC GC[ACGT]^[ACGT]GC
CfoI 916 GCGC GCG^C
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 16 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 899 GACTGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 824 ATGCAT ATGCA^T
Fnu4HI 339 GCTGC GC^[ACGT]GC
FspI 915 TGCGCA TGC^GCA
HhaI 916 GCGC GCG^C
HinP1I 916 GCGC G^CGC
HphI 50 TCACC GGTGA(8/7)
ItaI 339 GCTGC GC^[ACGT]GC
MaeI 697 CTAG C^TAG
MflI 299 AGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
NdeI 375 CATATG CA^TATG
NsiI 824 ATGCAT ATGCA^T
RcaI 404 TCATGA T^CATGA
RsaI 566 GTAC GT^AC
TseI 339 GCTGC G^C[AT]GC
XcmI 788 CCACATCACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 299 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AluI 71 AGCT AG^CT
723 AGCT
AvaII 471 GGTCC G^G[AT]CC
508 GGTCC
BanII 408 GAGCCC G[GA]GC[CT]^C
624 GGGCTC
BsaBI 154 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
802 GATAAATATC
BseRI 550 CTCCTC GAGGAG(10/8)
894 GAGGAG
BsgI 163 CTGCAC GTGCAG(16/14)
903 GTGCAG
BsrI 582 ACTGG ACTGG(1/-1)
850 CCAGT
BssKI 186 CCTGG ^CC[ACGT]GG
798 CCTGG
BstF5I 153 GGATG GGATG(2/0)
286 GGATG
BstNI 186 CCTGG CC^[AT]GG
798 CCTGG
DpnII 300 GATC ^GATC
538 GATC
DsaI 149 CCATGG C^C[GA][CT]GG
350 CCATGG
EcoRII 186 CCTGG ^CC[AT]GG
798 CCTGG
FokI 153 GGATG GGATG(9/13)
286 GGATG
MamI 154 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
802 GATAAATATC
MboI 300 GATC ^GATC
538 GATC
MvaI 186 CCTGG CC^[AT]GG
798 CCTGG
NcoI 149 CCATGG C^CATGG
350 CCATGG
NdeII 300 GATC ^GATC
538 GATC
NlaIV 548 GGCTCC GG[ACGT]^[ACGT]CC
625 GGCTCC
PflMI 462 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
576 CCAAGAACTGG
PleI 47 GACTC GAGTC(4/5)
611 GACTC
Sau3AI 300 GATC ^GATC
538 GATC
ScrFI 186 CCTGG CC^[ACGT]GG
798 CCTGG
SfaNI 154 GATGC GCATC(5/9)
823 GATGC
Tsp45I 595 GTCAC ^GT[GC]AC
730 GTCAC
Van91I 462 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
576 CCAAGAACTGG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
BmyI BsaJI BsiYI BslI Bsp1286I
Cfr13I DdeI EcoT14I HaeIII HinfI
MaeIII MboII MnlI MslI NlaIII
Sau96I StyI TfiI Tsp509I TspRI