Restriction map for
pFN21AE5958
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MspA1I MspI MunI MwoI
NaeI NarI NciI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 490 GTAGAC GT^[AC][GT]AC
AccII 59 CGCG CG^CG
AciI 60 GCGG CCGC(-3/-1)
AcsI 805 AAATTC [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
AlwI 66 GATCC GGATC(4/5)
ApoI 805 AAATTC [GA]^AATT[CT]
AseI 759 ATTAAT AT^TAAT
AsnI 759 ATTAAT AT^TAAT
BbsI 896 GAAGAC GAAGAC(2/6)
BbvI 339 GCTGC GCAGC(8/12)
BglII 299 AGATCT A^GATCT
BpuAI 896 GAAGAC GAAGAC(2/6)
BsiYI 363 CCGATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 363 CCGATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 404 TCATGA T^CATGA
BspMI 494 ACCTGC ACCTGC(4/8)
BsrDI 889 GCAATG GCAATG(2/0)
BstF5I 3 GGATG GGATG(2/0)
BstUI 59 CGCG CG^CG
BstXI 312 CCATGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 350 CCATGG C^C[GA][CT]GG
EarI 79 CTCTTC CTCTTC(1/4)
EcoT14I 350 CCATGG C^C[AT][AT]GG
Fnu4HI 339 GCTGC GC^[ACGT]GC
FokI 3 GGATG GGATG(9/13)
HphI 666 GGTGA GGTGA(8/7)
ItaI 339 GCTGC GC^[ACGT]GC
Ksp632I 79 CTCTTC CTCTTC(1/4)
MvnI 59 CGCG CG^CG
NcoI 350 CCATGG C^CATGG
NdeI 375 CATATG CA^TATG
PshBI 759 ATTAAT AT^TAAT
RcaI 404 TCATGA T^CATGA
RsaI 177 GTAC GT^AC
StyI 350 CCATGG C^C[AT][AT]GG
TaqI 546 TCGA T^CGA
TseI 339 GCTGC G^C[AT]GC
TthHB8I 546 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
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BalI 188 TGGCCA TGG^CCA
371 TGGCCA
BsaI 571 GAGACC GGTCTC(1/5)
24 GGTCTC
BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG
350 CCATGG
BsmAI 571 GAGAC GTCTC(1/5)
25 GTCTC
BsmFI 45 GGGAC GGGAC(10/14)
168 GTCCC
BsrI 558 ACTGG ACTGG(1/-1)
850 CCAGT
BssKI 42 CCTGG ^CC[ACGT]GG
443 CCTGG
BstNI 42 CCTGG CC^[AT]GG
443 CCTGG
BstYI 65 AGATCC [GA]^GATC[CT]
299 AGATCT
Cac8I 186 GCTGGC GC[ACGT]^[ACGT]GC
562 GCTTGC
Cfr13I 508 GGCCC G^G[ACGT]CC
754 GGGCC
DraIII 16 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
463 CACTCAGTG
EaeI 188 TGGCCA [CT]^GGCC[GA]
371 TGGCCA
EcoRII 42 CCTGG ^CC[AT]GG
443 CCTGG
MflI 65 AGATCC [GA]^GATC[CT]
299 AGATCT
MluNI 188 TGGCCA TGG^CCA
371 TGGCCA
MscI 188 TGGCCA TGG^CCA
371 TGGCCA
MseI 634 TTAA T^TAA
760 TTAA
MvaI 42 CCTGG CC^[AT]GG
443 CCTGG
PleI 47 GACTC GAGTC(4/5)
55 GACTC
Sau96I 508 GGCCC G^G[ACGT]CC
754 GGGCC
ScrFI 42 CCTGG CC^[ACGT]GG
443 CCTGG
SfaNI 823 GATGC GCATC(5/9)
902 GATGC
Tru9I 634 TTAA T^TAA
760 TTAA
Tsp45I 145 GTGAC ^GT[GC]AC
667 GTGAC
TspRI 95 CAGTG CA[GC]TG[ACGT][ACGT]^
467 CAGTG
XcmI 11 CCAATCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
200 CCATCATCAATTTGG
XhoII 65 AGATCC [GA]^GATC[CT]
299 AGATCT
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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AluI BseRI DdeI DpnII HaeIII
HinfI MaeIII MboI MboII MnlI
MslI NdeII NlaIII Sau3AI TfiI
Tsp509I