Restriction map for
pFN21AE5562
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoT22I FseI FspI HincII
HindII HindIII HpaI MamI MluI
MroI MseI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 597 GGTACC G^GTACC
AcsI 126 GAATTC [GA]^AATT[CT]
AflIII 116 ACGTGT A^C[GA][CT]GT
AluI 63 AGCT AG^CT
Alw44I 913 GTGCAC G^TGCAC
AlwNI 145 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 89 AGCGCT AGC^GCT
ApaLI 913 GTGCAC G^TGCAC
ApoI 126 GAATTC [GA]^AATT[CT]
Asp718I 597 GGTACC G^GTACC
BanII 373 GAGCCC G[GA]GC[CT]^C
BbeI 852 GGCGCC GGCGC^C
BclI 632 TGATCA T^GATCA
BfaI 322 CTAG C^TAG
BglI 902 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
Bsp1407I 884 TGTACA T^GTACA
BspDI 184 ATCGAT AT^CGAT
BsrGI 884 TGTACA T^GTACA
BssSI 562 CTCGTG CACGAG(-5/-1)
ClaI 184 ATCGAT AT^CGAT
DdeI 569 CTGAG C^T[ACGT]AG
EarI 765 GAAGAG CTCTTC(1/4)
Eco47III 89 AGCGCT AGC^GCT
EcoNI 1038 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 126 GAATTC G^AATTC
EcoRV 286 GATATC GAT^ATC
EcoT14I 260 CCTTGG C^C[AT][AT]GG
FbaI 632 TGATCA T^GATCA
HgaI 97 GACGC GACGC(5/10)
KasI 852 GGCGCC G^GCGCC
KpnI 597 GGTACC GGTAC^C
Ksp632I 765 GAAGAG CTCTTC(1/4)
KspI 897 CCGCGG CCGC^GG
MaeI 322 CTAG C^TAG
MaeII 116 ACGT A^CGT
MfeI 37 CAATTG C^AATTG
MunI 37 CAATTG C^AATTG
NarI 852 GGCGCC GG^CGCC
PshAI 720 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 897 CCGCGG CCGC^GG
ScaI 614 AGTACT AGT^ACT
SfoI 852 GGCGCC GGC^GCC
SmaI 772 CCCGGG CCC^GGG
SspBI 884 TGTACA T^GTACA
SspI 8 AATATT AAT^ATT
StyI 260 CCTTGG C^C[AT][AT]GG
TaqI 185 TCGA T^CGA
TthHB8I 185 TCGA T^CGA
XcmI 629 CCATGATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 772 CCCGGG C^CCGGG
XmaI 772 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 747 CGCG CG^CG
898 CGCG
AcyI 97 GACGCC G[GA]^CG[CT]C
852 GGCGCC
AvaI 772 CCCGGG C^[CT]CG[GA]G
1009 CCCGAG
BalI 827 TGGCCA TGG^CCA
1028 TGGCCA
BanI 597 GGTACC G^G[CT][GA]CC
852 GGCGCC
BbsI 550 GTCTTC GAAGAC(2/6)
1159 GTCTTC
BpmI 155 CTCCAG CTGGAG(16/14)
1039 CTGGAG
BpuAI 550 GTCTTC GAAGAC(2/6)
1159 GTCTTC
BsaHI 97 GACGCC G[GA]^CG[CT]C
852 GGCGCC
BseRI 342 GAGGAG GAGGAG(10/8)
1042 GAGGAG
BsoBI 772 CCCGGG C^[CT]CG[GA]G
1009 CCCGAG
BsrDI 139 CATTGC GCAATG(2/0)
1056 CATTGC
BstAPI 956 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
984 GCACCTCCTGC
BstUI 747 CGCG CG^CG
898 CGCG
BstXI 1031 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1202 CCAACTTCGTGG
BstYI 264 GGATCT [GA]^GATC[CT]
1034 AGATCC
DsaI 848 CCGTGG C^C[GA][CT]GG
897 CCGCGG
FauI 580 CCCGC CCCGC(4/6)
862 CCCGC
Hin1I 97 GACGCC G[GA]^CG[CT]C
852 GGCGCC
MflI 264 GGATCT [GA]^GATC[CT]
1034 AGATCC
MluNI 827 TGGCCA TGG^CCA
1028 TGGCCA
MscI 827 TGGCCA TGG^CCA
1028 TGGCCA
MslI 111 CACTAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1185 CATCCTGATG
MvnI 747 CGCG CG^CG
898 CGCG
PleI 934 GACTC GAGTC(4/5)
725 GAGTC
Tsp509I 38 AATT ^AATT
127 AATT
XhoII 264 GGATCT [GA]^GATC[CT]
1034 AGATCC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AlwI AspHI AvaII
BbvI BcnI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
TseI Tsp45I TspRI