Restriction map for
pFN21AE5982
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MscI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 554 TCCGGA T^CCGGA
AcsI 31 GAATTT [GA]^AATT[CT]
AfaI 216 GTAC GT^AC
AluI 299 AGCT AG^CT
AlwNI 230 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 508 GGGCCC GGGCC^C
ApoI 31 GAATTT [GA]^AATT[CT]
Asp700I 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 66 GGTCC G^G[AT]CC
BanII 508 GGGCCC G[GA]GC[CT]^C
BbvI 892 GCAGC GCAGC(8/12)
BciVI 425 GTATCC GTATCC(6/5)
BmyI 508 GGGCCC G[AGT]GC[ACT]^C
BsaAI 848 TACGTA [CT]AC^GT[GA]
BsaJI 20 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 554 TCCGGA [AT]^CCGG[AT]
BseAI 554 TCCGGA T^CCGGA
BseRI 583 GAGGAG GAGGAG(10/8)
BsmAI 416 GTCTC GTCTC(1/5)
BsmBI 415 CGTCTC CGTCTC(1/5)
Bsp1286I 508 GGGCCC G[AGT]GC[ACT]^C
BspEI 554 TCCGGA T^CCGGA
BspHI 404 TCATGA T^CATGA
BspMI 721 ACCTGC ACCTGC(4/8)
BsrDI 291 CATTGC GCAATG(2/0)
BsrI 43 ACTGG ACTGG(1/-1)
BstEII 909 GGTCACC G^GT[ACGT]ACC
BstPI 909 GGTCACC G^GT[ACGT]ACC
Cac8I 781 GCTCGC GC[ACGT]^[ACGT]GC
DdeI 690 CTCAG C^T[ACGT]AG
EcoO65I 909 GGTCACC G^GT[ACGT]ACC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
EcoT22I 288 ATGCAT ATGCA^T
Fnu4HI 892 GCAGC GC^[ACGT]GC
HapII 555 CCGG C^CGG
HpaII 555 CCGG C^CGG
ItaI 892 GCAGC GC^[ACGT]GC
MroI 554 TCCGGA T^CCGGA
MspI 555 CCGG C^CGG
MwoI 190 GCTAATCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 508 GGGCCC GG[ACGT]^[ACGT]CC
NsiI 288 ATGCAT ATGCA^T
RcaI 404 TCATGA T^CATGA
RsaI 216 GTAC GT^AC
SapI 300 GCTCTTC GCTCTTC(1/4)
SfaNI 287 GATGC GCATC(5/9)
SfcI 600 CTGTAG C^T[GA][CT]AG
SnaBI 848 TACGTA TAC^GTA
SpeI 72 ACTAGT A^CTAGT
SspI 837 AATATT AAT^ATT
StyI 20 CCAAGG C^C[AT][AT]GG
TaqI 492 TCGA T^CGA
TfiI 408 GAATC G^A[AT]TC
TseI 892 GCAGC G^C[AT]GC
TthHB8I 492 TCGA T^CGA
XbaI 150 TCTAGA T^CTAGA
XcmI 896 CCATGAGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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BpmI 59 CTCCAG CTGGAG(16/14)
444 CTGGAG
BsiYI 443 CCTGGAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCTTTCTGTGG
BslI 443 CCTGGAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCTTTCTGTGG
DpnII 597 GATC ^GATC
615 GATC
EarI 219 CTCTTC CTCTTC(1/4)
301 CTCTTC
Ksp632I 219 CTCTTC CTCTTC(1/4)
301 CTCTTC
MaeII 414 ACGT A^CGT
849 ACGT
MboI 597 GATC ^GATC
615 GATC
MseI 643 TTAA T^TAA
835 TTAA
NdeII 597 GATC ^GATC
615 GATC
PleI 57 GACTC GAGTC(4/5)
793 GACTC
Sau3AI 597 GATC ^GATC
615 GATC
Tru9I 643 TTAA T^TAA
835 TTAA
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
BfaI BssKI BstF5I BstNI Cfr13I
EcoRII FokI HaeIII HinfI HphI
MaeI MaeIII MboII MnlI MslI
MvaI NlaIII Sau96I ScrFI Tsp45I
Tsp509I TspRI