Restriction map for
pFN21AE2268
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 125 CGCG CG^CG
AfaI 285 GTAC GT^AC
Alw44I 181 GTGCAC G^TGCAC
AlwNI 303 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 181 GTGCAC G^TGCAC
AspHI 181 GTGCAC G[AT]GC[AT]^C
AvaII 354 GGTCC G^G[AT]CC
BalI 327 TGGCCA TGG^CCA
BamHI 50 GGATCC G^GATCC
BanI 30 GGTGCC G^G[CT][GA]CC
BfaI 25 CTAG C^TAG
BsiHKAI 181 GTGCAC G[AT]GC[AT]^C
BsrI 302 CCAGT ACTGG(1/-1)
BstUI 125 CGCG CG^CG
BstXI 54 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 50 GGATCC [GA]^GATC[CT]
Cac8I 252 GCCCGC GC[ACGT]^[ACGT]GC
DpnII 51 GATC ^GATC
DraII 142 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 324 CCATGG C^C[GA][CT]GG
EcoNI 122 CCTCGCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 142 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 324 CCATGG C^C[AT][AT]GG
FauI 253 CCCGC CCCGC(4/6)
HgaI 21 GCGTC GACGC(5/10)
HindIII 11 AAGCTT A^AGCTT
HphI 96 GGTGA GGTGA(8/7)
MaeI 25 CTAG C^TAG
MaeIII 97 GTGAC ^GT[ACGT]AC
MboI 51 GATC ^GATC
MboII 73 GAAGA GAAGA(8/7)
MflI 50 GGATCC [GA]^GATC[CT]
MluNI 327 TGGCCA TGG^CCA
MscI 327 TGGCCA TGG^CCA
MslI 174 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 125 CGCG CG^CG
NcoI 324 CCATGG C^CATGG
NdeII 51 GATC ^GATC
NlaIII 325 CATG CATG^
PflMI 302 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 337 GACTC GAGTC(4/5)
PshAI 99 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 285 GTAC GT^AC
Sau3AI 51 GATC ^GATC
StuI 58 AGGCCT AGG^CCT
StyI 324 CCATGG C^C[AT][AT]GG
TfiI 298 GATTC G^A[AT]TC
Tsp45I 97 GTGAC ^GT[GC]AC
Van91I 302 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 50 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 51 GATCC GGATC(4/5)
50 GGATC
AvaI 209 CCCGGG C^[CT]CG[GA]G
274 CCCGGG
BmyI 31 GTGCCC G[AGT]GC[ACT]^C
181 GTGCAC
BseRI 222 CTCCTC GAGGAG(10/8)
79 GAGGAG
BsoBI 209 CCCGGG C^[CT]CG[GA]G
274 CCCGGG
Bsp1286I 31 GTGCCC G[AGT]GC[ACT]^C
181 GTGCAC
HinfI 298 GATTC G^A[ACGT]TC
337 GACTC
MwoI 4 GCCTCCGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
183 GCACTTGTGGC
NlaIV 30 GGTGCC GG[ACGT]^[ACGT]CC
50 GGATCC
PvuII 247 CAGCTG CAG^CTG
349 CAGCTG
SmaI 209 CCCGGG CCC^GGG
274 CCCGGG
TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^
200 CAGTG
XmaCI 209 CCCGGG C^CCGGG
274 CCCGGG
XmaI 209 CCCGGG C^CCGGG
274 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstNI Cfr13I
EaeI EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MnlI MspA1I MspI
MvaI NciI Sau96I ScrFI SfaNI
TseI