Restriction map for
pFN21AE1894
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbsI BbvCI BciVI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcsI 341 AAATTC [GA]^AATT[CT]
AflIII 129 ACACGT A^C[GA][CT]GT
AlwI 1034 GGATC GGATC(4/5)
ApaI 269 GGGCCC GGGCC^C
ApoI 341 AAATTC [GA]^AATT[CT]
Asp700I 1227 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 928 GAGCTC G[AT]GC[AT]^C
AvaI 954 CTCGAG C^[CT]CG[GA]G
AviII 368 TGCGCA TGC^GCA
BanI 720 GGTGCC G^G[CT][GA]CC
BbrPI 130 CACGTG CAC^GTG
BcgI 489 CGAGGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 762 CCGGG CC^[GC]GG
BglII 579 AGATCT A^GATCT
BsaBI 673 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 880 GAGACC GGTCTC(1/5)
BsgI 780 CTGCAC GTGCAG(16/14)
BsiHKAI 928 GAGCTC G[AT]GC[AT]^C
BsoBI 954 CTCGAG C^[CT]CG[GA]G
Bsp1407I 179 TGTACA T^GTACA
BspHI 1238 TCATGA T^CATGA
BspMI 935 ACCTGC ACCTGC(4/8)
BsrDI 809 GCAATG GCAATG(2/0)
BsrGI 179 TGTACA T^GTACA
BstXI 242 CCACCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 579 AGATCT [GA]^GATC[CT]
DraI 637 TTTAAA TTT^AAA
DsaI 785 CCGTGG C^C[GA][CT]GG
EarI 823 CTCTTC CTCTTC(1/4)
EcoT14I 248 CCTTGG C^C[AT][AT]GG
FspI 368 TGCGCA TGC^GCA
HapII 762 CCGG C^CGG
HpaII 762 CCGG C^CGG
Ksp632I 823 CTCTTC CTCTTC(1/4)
MamI 673 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 579 AGATCT [GA]^GATC[CT]
MseI 638 TTAA T^TAA
MslI 783 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 762 CCGG C^CGG
NciI 762 CCGGG CC^[GC]GG
PaeR7I 954 CTCGAG C^TCGAG
PmaCI 130 CACGTG CAC^GTG
PmlI 130 CACGTG CAC^GTG
PpuMI 590 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1238 TCATGA T^CATGA
SacI 928 GAGCTC GAGCT^C
SspBI 179 TGTACA T^GTACA
StyI 248 CCTTGG C^C[AT][AT]GG
TaqI 955 TCGA T^CGA
Tru9I 638 TTAA T^TAA
Tsp45I 1249 GTCAC ^GT[GC]AC
TthHB8I 955 TCGA T^CGA
XhoI 954 CTCGAG C^TCGAG
XhoII 579 AGATCT [GA]^GATC[CT]
XmnI 1227 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 180 GTAC GT^AC
801 GTAC
BfaI 563 CTAG C^TAG
1054 CTAG
BpmI 985 CTCCAG CTGGAG(16/14)
907 CTGGAG
BsaAI 130 CACGTG [CT]AC^GT[GA]
547 TACGTG
BstAPI 360 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1114 GCATCTGCTGC
BstF5I 828 CATCC GGATG(2/0)
465 GGATG
Cac8I 814 GCCAGC GC[ACGT]^[ACGT]GC
1263 GCAAGC
CfoI 369 GCGC GCG^C
818 GCGC
FokI 828 CATCC GGATG(9/13)
465 GGATG
HhaI 369 GCGC GCG^C
818 GCGC
HinP1I 369 GCGC G^CGC
818 GCGC
MaeI 563 CTAG C^TAG
1054 CTAG
MaeIII 12 GTTAC ^GT[ACGT]AC
1249 GTCAC
PstI 397 CTGCAG CTGCA^G
521 CTGCAG
RsaI 180 GTAC GT^AC
801 GTAC
StuI 303 AGGCCT AGG^CCT
491 AGGCCT
TfiI 96 GAATC G^A[AT]TC
294 GATTC
Tsp509I 342 AATT ^AATT
625 AATT
TspRI 230 CACTG CA[GC]TG[ACGT][ACGT]^
501 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BclI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FbaI Fnu4HI HaeIII
HinfI HphI ItaI MaeII MboI
MboII MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TseI