Restriction map for
pFN21AB9939
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AspI AvaI AviII AvrII
BalI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HpaI KspI MaeIII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1176 GGTACC G^GTACC
AcyI 3 GGCGCC G[GA]^CG[CT]C
AlwNI 1272 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1176 GGTACC G^GTACC
AspHI 247 GAGCAC G[AT]GC[AT]^C
BamHI 1104 GGATCC G^GATCC
BanII 736 GAGCCC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BciVI 1413 GTATCC GTATCC(6/5)
BcnI 24 CCGGG CC^[GC]GG
BglI 6 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaWI 447 TCCGGT [AT]^CCGG[AT]
BsiHKAI 247 GAGCAC G[AT]GC[AT]^C
BspMI 949 ACCTGC ACCTGC(4/8)
BsrBI 10 GAGCGG CCGCTC(-3/-3)
BsrDI 1437 CATTGC GCAATG(2/0)
BstAPI 1271 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1378 CCAGTGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 63 CCGTGG C^C[GA][CT]GG
FauI 48 GCGGG CCCGC(4/6)
HaeIII 1133 GGCC GG^CC
HgaI 1280 GCGTC GACGC(5/10)
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HindIII 1007 AAGCTT A^AGCTT
KasI 3 GGCGCC G^GCGCC
KpnI 1176 GGTACC GGTAC^C
MaeII 251 ACGT A^CGT
NarI 3 GGCGCC GG^CGCC
NciI 24 CCGGG CC^[GC]GG
NspI 661 GCATGC [GA]CATG^[CT]
PflMI 1507 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1353 GACTC GAGTC(4/5)
SapI 94 GCTCTTC GCTCTTC(1/4)
SfcI 242 CTATAG C^T[GA][CT]AG
SfoI 3 GGCGCC GGC^GCC
SphI 661 GCATGC GCATG^C
SspI 769 AATATT AAT^ATT
TfiI 1018 GATTC G^A[AT]TC
Van91I 1507 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AseI 145 ATTAAT AT^TAAT
772 ATTAAT
AsnI 145 ATTAAT AT^TAAT
772 ATTAAT
Asp700I 91 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
904 GAAAAACTTC
BanI 3 GGCGCC G^G[CT][GA]CC
1176 GGTACC
BseRI 443 CTCCTC GAGGAG(10/8)
838 GAGGAG
BsmI 217 GAATGC GAATGC(1/-1)
1477 GAATGC
Bsp1407I 293 TGTACA T^GTACA
1222 TGTACA
BsrGI 293 TGTACA T^GTACA
1222 TGTACA
BstYI 594 AGATCC [GA]^GATC[CT]
1104 GGATCC
Cac8I 53 GCTTGC GC[ACGT]^[ACGT]GC
661 GCATGC
CfoI 4 GCGC GCG^C
28 GCGC
DraII 418 AGGACCC [GA]G^G[ACGT]CC[CT]
786 AGGTCCT
EarI 95 CTCTTC CTCTTC(1/4)
835 GAAGAG
EcoO109I 418 AGGACCC [GA]G^G[ACGT]CC[CT]
786 AGGTCCT
EcoRI 1162 GAATTC G^AATTC
1211 GAATTC
EcoT14I 1129 CCAAGG C^C[AT][AT]GG
1341 CCAAGG
HaeII 3 GGCGCC [GA]GCGC^[CT]
27 GGCGCT
HhaI 4 GCGC GCG^C
28 GCGC
HinP1I 4 GCGC G^CGC
28 GCGC
HinfI 1018 GATTC G^A[ACGT]TC
1353 GACTC
Ksp632I 95 CTCTTC CTCTTC(1/4)
835 GAAGAG
MflI 594 AGATCC [GA]^GATC[CT]
1104 GGATCC
NdeI 986 CATATG CA^TATG
1391 CATATG
PpuMI 418 AGGACCC [GA]G^G[AT]CC[CT]
786 AGGTCCT
PshBI 145 ATTAAT AT^TAAT
772 ATTAAT
ScaI 745 AGTACT AGT^ACT
1217 AGTACT
SexAI 887 ACCAGGT A^CC[AT]GGT
1092 ACCTGGT
SspBI 293 TGTACA T^GTACA
1222 TGTACA
StyI 1129 CCAAGG C^C[AT][AT]GG
1341 CCAAGG
XhoII 594 AGATCC [GA]^GATC[CT]
1104 GGATCC
XmnI 91 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
904 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BmyI BpuAI BsaJI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HapII HpaII HphI ItaI MaeI
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp509I TspRI