Restriction map for
pFN21AE9682
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AciI 313 CCGC CCGC(-3/-1)
AfaI 347 GTAC GT^AC
Asp700I 947 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 14 CCCGAG C^[CT]CG[GA]G
AvaII 622 GGTCC G^G[AT]CC
BamHI 579 GGATCC G^GATCC
BbvI 83 GCAGC GCAGC(8/12)
BsaAI 806 TACGTG [CT]AC^GT[GA]
BseRI 484 GAGGAG GAGGAG(10/8)
BsgI 751 CTGCAC GTGCAG(16/14)
BsoBI 14 CCCGAG C^[CT]CG[GA]G
BsrDI 688 GCAATG GCAATG(2/0)
DraIII 709 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Fnu4HI 83 GCAGC GC^[ACGT]GC
HphI 793 GGTGA GGTGA(8/7)
ItaI 83 GCAGC GC^[ACGT]GC
MaeII 807 ACGT A^CGT
MslI 40 CATCACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 313 CCGCTG C[AC]G^C[GT]G
MwoI 286 GCTGGAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 877 CATG CATG^
RsaI 347 GTAC GT^AC
SfaNI 262 GATGC GCATC(5/9)
SspI 950 AATATT AAT^ATT
StuI 898 AGGCCT AGG^CCT
TseI 83 GCAGC G^C[AT]GC
XmnI 947 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 341 AAATTC [GA]^AATT[CT]
413 AAATTC
AlwI 580 GATCC GGATC(4/5)
579 GGATC
ApoI 341 AAATTC [GA]^AATT[CT]
413 AAATTC
AspHI 108 GAGCTC G[AT]GC[AT]^C
373 GAGCTC
BanII 108 GAGCTC G[GA]GC[CT]^C
373 GAGCTC
BfaI 481 CTAG C^TAG
761 CTAG
BglII 648 AGATCT A^GATCT
763 AGATCT
BmyI 108 GAGCTC G[AGT]GC[ACT]^C
373 GAGCTC
BsaI 31 GAGACC GGTCTC(1/5)
117 GGTCTC
BsiHKAI 108 GAGCTC G[AT]GC[AT]^C
373 GAGCTC
BsmAI 31 GAGAC GTCTC(1/5)
118 GTCTC
Bsp1286I 108 GAGCTC G[AGT]GC[ACT]^C
373 GAGCTC
Cac8I 315 GCTGGC GC[ACGT]^[ACGT]GC
428 GCCAGC
Cfr13I 622 GGTCC G^G[ACGT]CC
703 GGGCC
EarI 505 GAAGAG CTCTTC(1/4)
769 GAAGAG
HaeIII 704 GGCC GG^CC
899 GGCC
HindIII 419 AAGCTT A^AGCTT
919 AAGCTT
HinfI 362 GAATC G^A[ACGT]TC
385 GAATC
Ksp632I 505 GAAGAG CTCTTC(1/4)
769 GAAGAG
MaeI 481 CTAG C^TAG
761 CTAG
SacI 108 GAGCTC GAGCT^C
373 GAGCTC
Sau96I 622 GGTCC G^G[ACGT]CC
703 GGGCC
SfcI 366 CTATAG C^T[GA][CT]AG
937 CTACAG
TfiI 362 GAATC G^A[AT]TC
385 GAATC
TspRI 102 CACTG CA[GC]TG[ACGT][ACGT]^
756 CAGTG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BbsI BpuAI BsaJI BsrI
BstF5I BstYI DdeI DpnII FokI
MboI MboII MflI MnlI MseI
NdeII NlaIV Sau3AI Tru9I Tsp509I
XhoII