Restriction map for
pFN21AE5389
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HinfI HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NcoI NdeII NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TfiI
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AfaI 472 GTAC GT^AC
AlwNI 152 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 679 GAGCAC G[AT]GC[AT]^C
AspI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 754 GGACC G^G[AT]CC
BanII 160 GGGCTC G[GA]GC[CT]^C
BbvI 287 GCTGC GCAGC(8/12)
BlpI 615 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 615 GCTCAGC GC^T[ACGT]AGC
BsaI 597 GGTCTC GGTCTC(1/5)
BsiHKAI 679 GAGCAC G[AT]GC[AT]^C
Bsp1407I 471 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BspMI 756 ACCTGC ACCTGC(4/8)
BsrBI 323 GAGCGG CCGCTC(-3/-3)
BsrFI 380 GCCGGC [GA]^CCGG[CT]
BsrGI 471 TGTACA T^GTACA
BstXI 236 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 615 GCTCAGC GC^T[ACGT]AGC
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
EcoRI 31 GAATTC G^AATTC
FauI 325 GCGGG CCCGC(4/6)
Fnu4HI 287 GCTGC GC^[ACGT]GC
HapII 381 CCGG C^CGG
HincII 908 GTCAAC GT[CT]^[GA]AC
HindII 908 GTCAAC GT[CT]^[GA]AC
HindIII 884 AAGCTT A^AGCTT
HpaII 381 CCGG C^CGG
ItaI 287 GCTGC GC^[ACGT]GC
MaeIII 25 GTGAC ^GT[ACGT]AC
MslI 18 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 797 CAGCTG C[AC]G^C[GT]G
MspI 381 CCGG C^CGG
NaeI 380 GCCGGC GCC^GGC
NdeI 913 CATATG CA^TATG
NgoMIV 380 GCCGGC G^CCGGC
NlaIV 231 GGTTCC GG[ACGT]^[ACGT]CC
NspI 715 ACATGC [GA]CATG^[CT]
PflFI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 797 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
RsaI 472 GTAC GT^AC
SfcI 279 CTATAG C^T[GA][CT]AG
SmlI 59 CTCAAG C^T[CT][GA]AG
SspBI 471 TGTACA T^GTACA
TaqI 521 TCGA T^CGA
TseI 287 GCTGC G^C[AT]GC
Tsp45I 25 GTGAC ^GT[GC]AC
Tth111I 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 521 TCGA T^CGA
XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
325 GCGG
AcsI 31 GAATTC [GA]^AATT[CT]
123 AAATTT
ApoI 31 GAATTC [GA]^AATT[CT]
123 AAATTT
BfaI 88 CTAG C^TAG
413 CTAG
BmyI 160 GGGCTC G[AGT]GC[ACT]^C
679 GAGCAC
BseRI 340 CTCCTC GAGGAG(10/8)
684 CTCCTC
BsgI 490 CTGCAC GTGCAG(16/14)
564 CTGCAC
BsmAI 57 GTCTC GTCTC(1/5)
598 GTCTC
Bsp1286I 160 GGGCTC G[AGT]GC[ACT]^C
679 GAGCAC
BsrI 204 ACTGG ACTGG(1/-1)
54 CCAGT
EcoT22I 209 ATGCAT ATGCA^T
425 ATGCAT
MaeI 88 CTAG C^TAG
413 CTAG
MseI 94 TTAA T^TAA
904 TTAA
NsiI 209 ATGCAT ATGCA^T
425 ATGCAT
Tru9I 94 TTAA T^TAA
904 TTAA
TspRI 20 CAGTG CA[GC]TG[ACGT][ACGT]^
606 CAGTG
XcmI 236 CCAGGATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
569 CCAATACCTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII FokI HaeIII HphI MboII
MnlI MvaI MwoI NlaIII Sau96I
ScrFI SfaNI Tsp509I