Restriction map for
pFN21AE5377
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 677 GGTACC G^GTACC
AfaI 678 GTAC GT^AC
Alw44I 460 GTGCAC G^TGCAC
AlwNI 586 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 460 GTGCAC G^TGCAC
Asp718I 677 GGTACC G^GTACC
AvaII 508 GGTCC G^G[AT]CC
BanI 677 GGTACC G^G[CT][GA]CC
BbsI 100 GTCTTC GAAGAC(2/6)
BlpI 186 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 186 GCTTAGC GC^T[ACGT]AGC
BpuAI 100 GTCTTC GAAGAC(2/6)
BseRI 634 CTCCTC GAGGAG(10/8)
BsmAI 646 GTCTC GTCTC(1/5)
BspHI 404 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrDI 597 CATTGC GCAATG(2/0)
Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC
CelII 186 GCTTAGC GC^T[ACGT]AGC
EcoT14I 350 CCATGG C^C[AT][AT]GG
HaeIII 779 GGCC GG^CC
HincII 489 GTTGAC GT[CT]^[GA]AC
HindII 489 GTTGAC GT[CT]^[GA]AC
HphI 138 GGTGA GGTGA(8/7)
KpnI 677 GGTACC GGTAC^C
NcoI 350 CCATGG C^CATGG
NdeI 375 CATATG CA^TATG
RcaI 404 TCATGA T^CATGA
RsaI 678 GTAC GT^AC
SfcI 583 CTACAG C^T[GA][CT]AG
SspI 663 AATATT AAT^ATT
StyI 350 CCATGG C^C[AT][AT]GG
TaqI 693 TCGA T^CGA
TthHB8I 693 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
806 AAATTC
AlwI 213 GATCC GGATC(4/5)
695 GATCC
ApoI 31 GAATTT [GA]^AATT[CT]
806 AAATTC
AspHI 418 GTGCTC G[AT]GC[AT]^C
460 GTGCAC
BanII 408 GAGCCC G[GA]GC[CT]^C
610 GGGCTC
BbvI 886 GCAGC GCAGC(8/12)
685 GCTGC
BfaI 470 CTAG C^TAG
689 CTAG
BsiHKAI 418 GTGCTC G[AT]GC[AT]^C
460 GTGCAC
BsmFI 45 GGGAC GGGAC(10/14)
509 GTCCC
BsrI 424 ACTGG ACTGG(1/-1)
621 ACTGG
BstF5I 579 CATCC GGATG(2/0)
286 GGATG
Cfr13I 508 GGTCC G^G[ACGT]CC
779 GGCCC
DpnII 213 GATC ^GATC
695 GATC
DsaI 350 CCATGG C^C[GA][CT]GG
412 CCACGG
Fnu4HI 685 GCTGC GC^[ACGT]GC
886 GCAGC
FokI 579 CATCC GGATG(9/13)
286 GGATG
ItaI 685 GCTGC GC^[ACGT]GC
886 GCAGC
MaeI 470 CTAG C^TAG
689 CTAG
MaeII 264 ACGT A^CGT
773 ACGT
MboI 213 GATC ^GATC
695 GATC
MwoI 220 GCTTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
420 GCTCACTGGGC
NdeII 213 GATC ^GATC
695 GATC
PflMI 15 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
272 CCATCTCATGG
Sau3AI 213 GATC ^GATC
695 GATC
Sau96I 508 GGTCC G^G[ACGT]CC
779 GGCCC
TfiI 89 GATTC G^A[AT]TC
154 GATTC
TseI 685 GCTGC G^C[AT]GC
886 GCAGC
Van91I 15 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
272 CCATCTCATGG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI DdeI EcoRII
HinfI MaeIII MboII MnlI MseI
MslI MvaI NlaIII NlaIV PleI
ScrFI Tru9I Tsp45I Tsp509I TspRI