Restriction map for
pFN21AE5906
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MflI MluI MroI
MseI MspA1I MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 523 GTAGAC GT^[AC][GT]AC
AfaI 180 GTAC GT^AC
AflIII 212 ACATGT A^C[GA][CT]GT
Alw44I 463 GTGCAC G^TGCAC
ApaLI 463 GTGCAC G^TGCAC
AvaII 3 GGACC G^G[AT]CC
BfaI 199 CTAG C^TAG
BspLU11I 212 ACATGT A^CATGT
BsrFI 691 GCCGGT [GA]^CCGG[CT]
BsrI 322 ACTGG ACTGG(1/-1)
BstEII 121 GGTAACC G^GT[ACGT]ACC
BstPI 121 GGTAACC G^GT[ACGT]ACC
BstXI 110 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 691 GCCGGT [GA]^CCGG[CT]
DrdI 574 GACACCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 353 CCATGG C^C[GA][CT]GG
EcoO65I 121 GGTAACC G^GT[ACGT]ACC
FauI 432 GCGGG CCCGC(4/6)
HapII 692 CCGG C^CGG
HgaI 744 GACGC GACGC(5/10)
HinfI 47 GACTC G^A[ACGT]TC
HpaII 692 CCGG C^CGG
MaeI 199 CTAG C^TAG
MslI 735 CATGGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 692 CCGG C^CGG
MwoI 223 GCCTCATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 353 CCATGG C^CATGG
NdeI 378 CATATG CA^TATG
NspI 212 ACATGT [GA]CATG^[CT]
PleI 47 GACTC GAGTC(4/5)
RsaI 180 GTAC GT^AC
SfaNI 425 GCATC GCATC(5/9)
SmlI 624 CTTGAG C^T[CT][GA]AG
XcmI 470 CCATCAGTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 432 GCGG CCGC(-3/-1)
780 GCGG
AlwI 154 GATCC GGATC(4/5)
663 GATCC
AspHI 340 GTGCTC G[AT]GC[AT]^C
463 GTGCAC
BalI 107 TGGCCA TGG^CCA
374 TGGCCA
BmyI 340 GTGCTC G[AGT]GC[ACT]^C
463 GTGCAC
BsaJI 20 CCTTGG C^C[ACGT][ACGT]GG
353 CCATGG
BsiHKAI 340 GTGCTC G[AT]GC[AT]^C
463 GTGCAC
Bsp1286I 340 GTGCTC G[AGT]GC[ACT]^C
463 GTGCAC
BssKI 207 CCTGG ^CC[ACGT]GG
345 CCTGG
BstF5I 701 CATCC GGATG(2/0)
289 GGATG
BstNI 207 CCTGG CC^[AT]GG
345 CCTGG
EaeI 107 TGGCCA [CT]^GGCC[GA]
374 TGGCCA
EcoRII 207 CCTGG ^CC[AT]GG
345 CCTGG
EcoT14I 20 CCTTGG C^C[AT][AT]GG
353 CCATGG
FokI 701 CATCC GGATG(9/13)
289 GGATG
HphI 552 GGTGA GGTGA(8/7)
918 GGTGA
MluNI 107 TGGCCA TGG^CCA
374 TGGCCA
MscI 107 TGGCCA TGG^CCA
374 TGGCCA
MvaI 207 CCTGG CC^[AT]GG
345 CCTGG
ScrFI 207 CCTGG CC^[ACGT]GG
345 CCTGG
StyI 20 CCTTGG C^C[AT][AT]GG
353 CCATGG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI ApoI BbvI BclI
BseRI BsgI BsiYI BslI Cac8I
Cfr13I DdeI DpnII FbaI Fnu4HI
HaeIII ItaI MaeIII MboI MboII
MnlI NdeII NlaIII NlaIV Sau3AI
Sau96I TseI Tsp45I Tsp509I TspRI