Restriction map for
pFN21AE5494
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BspEI BspLU11I BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI HaeII HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NruI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 592 GTAGAC GT^[AC][GT]AC
AcsI 103 GAATTT [GA]^AATT[CT]
ApoI 103 GAATTT [GA]^AATT[CT]
AspI 874 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 92 CTCGGG C^[CT]CG[GA]G
AviII 335 TGCGCA TGC^GCA
BanII 136 GAGCTC G[GA]GC[CT]^C
BbvI 958 GCAGC GCAGC(8/12)
BglI 358 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI 422 CCATGG C^C[ACGT][ACGT]GG
BsiYI 571 CCATTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 571 CCATTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 553 GCATTC GAATGC(1/-1)
BsoBI 92 CTCGGG C^[CT]CG[GA]G
Bsp1407I 929 TGTACA T^GTACA
BspDI 69 ATCGAT AT^CGAT
BspHI 476 TCATGA T^CATGA
BsrBI 780 CCGCTC CCGCTC(-3/-3)
BsrGI 929 TGTACA T^GTACA
BstXI 542 CCAGCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 336 GCGC GCG^C
ClaI 69 ATCGAT AT^CGAT
DpnII 19 GATC ^GATC
DraI 915 TTTAAA TTT^AAA
DsaI 422 CCATGG C^C[GA][CT]GG
EcoT14I 422 CCATGG C^C[AT][AT]GG
EcoT22I 831 ATGCAT ATGCA^T
Fnu4HI 958 GCAGC GC^[ACGT]GC
FspI 335 TGCGCA TGC^GCA
HhaI 336 GCGC GCG^C
HinP1I 336 GCGC G^CGC
HincII 871 GTTGAC GT[CT]^[GA]AC
HindII 871 GTTGAC GT[CT]^[GA]AC
HphI 96 GGTGA GGTGA(8/7)
ItaI 958 GCAGC GC^[ACGT]GC
MaeII 281 ACGT A^CGT
MboI 19 GATC ^GATC
NcoI 422 CCATGG C^CATGG
NdeII 19 GATC ^GATC
NsiI 831 ATGCAT ATGCA^T
PflFI 874 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 571 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 978 GAGTC GAGTC(4/5)
RcaI 476 TCATGA T^CATGA
SacI 136 GAGCTC GAGCT^C
Sau3AI 19 GATC ^GATC
SfaNI 355 GATGC GCATC(5/9)
SfcI 1007 CTGTAG C^T[GA][CT]AG
SspBI 929 TGTACA T^GTACA
StyI 422 CCATGG C^C[AT][AT]GG
TaqI 70 TCGA T^CGA
TseI 958 GCAGC G^C[AT]GC
Tth111I 874 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 70 TCGA T^CGA
Van91I 571 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 231 CCGC CCGC(-3/-1)
780 CCGC
AfaI 249 GTAC GT^AC
930 GTAC
AvaII 580 GGTCC G^G[AT]CC
826 GGACC
BseRI 706 CTCCTC GAGGAG(10/8)
756 CTCCTC
BsmAI 79 GAGAC GTCTC(1/5)
718 GTCTC
BsmFI 117 GGGAC GGGAC(10/14)
57 GTCCC
BsrI 394 ACTGG ACTGG(1/-1)
546 CCAGT
BssKI 363 CCTGG ^CC[ACGT]GG
515 CCTGG
BstF5I 207 CATCC GGATG(2/0)
531 CATCC
BstNI 363 CCTGG CC^[AT]GG
515 CCTGG
EarI 388 CTCTTC CTCTTC(1/4)
975 GAAGAG
EcoRII 363 CCTGG ^CC[AT]GG
515 CCTGG
FauI 230 CCCGC CCCGC(4/6)
779 CCCGC
FokI 207 CATCC GGATG(9/13)
531 CATCC
Ksp632I 388 CTCTTC CTCTTC(1/4)
975 GAAGAG
MvaI 363 CCTGG CC^[AT]GG
515 CCTGG
RsaI 249 GTAC GT^AC
930 GTAC
ScrFI 363 CCTGG CC^[ACGT]GG
515 CCTGG
TfiI 226 GATTC G^A[AT]TC
750 GAATC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AspHI BfaI BmyI BsiHKAI
Bsp1286I Cac8I Cfr13I DdeI HaeIII
HinfI MaeI MaeIII MboII MnlI
MseI MslI MwoI NlaIII Sau96I
Tru9I Tsp45I Tsp509I TspRI