##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          247  GTAGAC               GT^[AC][GT]AC
AciI          448  GCGG                 CCGC(-3/-1)
AcsI          805  AAATTT               [GA]^AATT[CT]
ApoI          805  AAATTT               [GA]^AATT[CT]
AspHI         262  GAGCAC               G[AT]GC[AT]^C
BbsI          788  GAAGAC               GAAGAC(2/6)
BbvI          886  GCAGC                GCAGC(8/12)
BciVI         570  GGATAC               GTATCC(6/5)
BfaI          118  CTAG                 C^TAG
BmyI          262  GAGCAC               G[AGT]GC[ACT]^C
BpmI          687  CTCCAG               CTGGAG(16/14)
BpuAI         788  GAAGAC               GAAGAC(2/6)
BsaWI         410  ACCGGA               [AT]^CCGG[AT]
BsgI          388  CTGCAC               GTGCAG(16/14)
BsiHKAI       262  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      262  GAGCAC               G[AGT]GC[ACT]^C
BspDI          18  ATCGAT               AT^CGAT
BsrDI         122  GCAATG               GCAATG(2/0)
BsrI          689  CCAGT                ACTGG(1/-1)
BstXI         512  CCAATGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI           18  ATCGAT               AT^CGAT
DpnII         666  GATC                 ^GATC
DraII         682  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      682  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       545  CCTTGG               C^C[AT][AT]GG
Fnu4HI        886  GCAGC                GC^[ACGT]GC
HapII         411  CCGG                 C^CGG
HpaII         411  CCGG                 C^CGG
HphI          549  GGTGA                GGTGA(8/7)
ItaI          886  GCAGC                GC^[ACGT]GC
MaeI          118  CTAG                 C^TAG
MboI          666  GATC                 ^GATC
MspI          411  CCGG                 C^CGG
NdeII         666  GATC                 ^GATC
NlaIV         508  GGTCCC               GG[ACGT]^[ACGT]CC
PflMI         499  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         682  AGGTCCT              [GA]G^G[AT]CC[CT]
Sau3AI        666  GATC                 ^GATC
StyI          545  CCTTGG               C^C[AT][AT]GG
TaqI           19  TCGA                 T^CGA
TfiI           16  GAATC                G^A[AT]TC
TseI          886  GCAGC                G^C[AT]GC
TthHB8I        19  TCGA                 T^CGA
Van91I        499  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BspMI         494  ACCTGC               ACCTGC(4/8)
              680  GCAGGT              
Cac8I         558  GCTGGC               GC[ACGT]^[ACGT]GC
              562  GCTTGC              
EcoRV         208  GATATC               GAT^ATC
              615  GATATC              
MaeIII        106  GTGAC                ^GT[ACGT]AC
              536  GTGAC               
MslI            7  CACACAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              729  CATCACAGTG          
PleI           47  GACTC                GAGTC(4/5)
              154  GACTC               
SfaNI         240  GATGC                GCATC(5/9)
              677  GATGC               
SfcI           95  CTATAG               C^T[GA][CT]AG
              393  CTACAG              
Tsp45I        106  GTGAC                ^GT[GC]AC
              536  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AfaI        AluI        AvaII       BsaJI       BsiYI       
BslI        BsmFI       BssKI       BstNI       Cfr13I      
DdeI        EcoRII      HaeIII      HinfI       MboII       
MnlI        MseI        MvaI        NdeI        NlaIII      
RsaI        Sau96I      ScrFI       Tru9I       Tsp509I     
TspRI