Restriction map for
pFN21AE6074
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI AlwI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI TfiI Tth111I Van91I XbaI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AlwNI 282 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 915 CCTGAGG CC^T[ACGT]AGG
AvaI 545 CTCGAG C^[CT]CG[GA]G
BbsI 684 GTCTTC GAAGAC(2/6)
BcgI 412 CGAAGGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 946 CTAG C^TAG
BpuAI 684 GTCTTC GAAGAC(2/6)
BsaI 268 GAGACC GGTCTC(1/5)
BsgI 163 CTGCAC GTGCAG(16/14)
BsmAI 268 GAGAC GTCTC(1/5)
BsmFI 45 GGGAC GGGAC(10/14)
BsoBI 545 CTCGAG C^[CT]CG[GA]G
BspHI 404 TCATGA T^CATGA
BspMI 494 ACCTGC ACCTGC(4/8)
BsrDI 694 GCAATG GCAATG(2/0)
BssSI 265 CACGAG CACGAG(-5/-1)
Bst1107I 102 GTATAC GTA^TAC
BstZ17I 102 GTATAC GTA^TAC
Bsu36I 915 CCTGAGG CC^T[ACGT]AGG
Eco81I 915 CCTGAGG CC^T[ACGT]AGG
EcoNI 356 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 611 GAATTC G^AATTC
EcoT14I 922 CCAAGG C^C[AT][AT]GG
HincII 205 GTTGAC GT[CT]^[GA]AC
HindII 205 GTTGAC GT[CT]^[GA]AC
MaeI 946 CTAG C^TAG
MseI 469 TTAA T^TAA
MslI 768 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 689 CAGCTG C[AC]G^C[GT]G
NdeI 375 CATATG CA^TATG
NspI 418 ACATGC [GA]CATG^[CT]
PaeR7I 545 CTCGAG C^TCGAG
PshAI 208 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 689 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SfaNI 761 GCATC GCATC(5/9)
SfcI 51 CTGTAG C^T[GA][CT]AG
SpeI 945 ACTAGT A^CTAGT
SspI 741 AATATT AAT^ATT
StyI 922 CCAAGG C^C[AT][AT]GG
Tru9I 469 TTAA T^TAA
XhoI 545 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 102 GTATAC GT^[AC][GT]AC
520 GTAGAC
AfaI 37 GTAC GT^AC
177 GTAC
Alw44I 260 GTGCAC G^TGCAC
460 GTGCAC
ApaLI 260 GTGCAC G^TGCAC
460 GTGCAC
Asp700I 191 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
611 GAATTCTTTC
AspHI 260 GTGCAC G[AT]GC[AT]^C
460 GTGCAC
AvaII 508 GGACC G^G[AT]CC
754 GGACC
BmyI 260 GTGCAC G[AGT]GC[ACT]^C
460 GTGCAC
BpmI 67 CTCCAG CTGGAG(16/14)
323 CTGGAG
BsaJI 443 CCTGGG C^C[ACGT][ACGT]GG
922 CCAAGG
BsiHKAI 260 GTGCAC G[AT]GC[AT]^C
460 GTGCAC
BsiYI 356 CCTATGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCTTTTTGTGG
BslI 356 CCTATGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCTTTTTGTGG
Bsp1286I 260 GTGCAC G[AGT]GC[ACT]^C
460 GTGCAC
BsrI 322 ACTGG ACTGG(1/-1)
474 CCAGT
BssKI 443 CCTGG ^CC[ACGT]GG
567 CCTGG
BstNI 443 CCTGG CC^[AT]GG
567 CCTGG
DpnII 438 GATC ^GATC
538 GATC
EcoRII 443 CCTGG ^CC[AT]GG
567 CCTGG
MboI 438 GATC ^GATC
538 GATC
MvaI 443 CCTGG CC^[AT]GG
567 CCTGG
MwoI 220 GCTTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
691 GCTGCAATGGC
NdeII 438 GATC ^GATC
538 GATC
NlaIII 405 CATG CATG^
419 CATG
RsaI 37 GTAC GT^AC
177 GTAC
Sau3AI 438 GATC ^GATC
538 GATC
ScrFI 443 CCTGG CC^[ACGT]GG
567 CCTGG
SmlI 545 CTCGAG C^T[CT][GA]AG
679 CTCAAG
TaqI 349 TCGA T^CGA
546 TCGA
TthHB8I 349 TCGA T^CGA
546 TCGA
XcmI 11 CCAATTCTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
788 CCATCTCTCCTTTGG
XmnI 191 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
611 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AluI ApoI BbvI Cac8I
Cfr13I DdeI Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
PleI Sau96I TseI Tsp45I Tsp509I
TspRI