##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        TaqI        Tru9I       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflIII        224  ACATGT               A^C[GA][CT]GT
AocI          945  CCTGAGG              CC^T[ACGT]AGG
BamHI         691  GGATCC               G^GATCC
BanII         825  GAGCCC               G[GA]GC[CT]^C
BbvI          940  GCAGC                GCAGC(8/12)
BclI          405  TGATCA               T^GATCA
BglII         116  AGATCT               A^GATCT
BmyI          825  GAGCCC               G[AGT]GC[ACT]^C
BsaI          770  GGTCTC               GGTCTC(1/5)
BsmI          376  GAATGC               GAATGC(1/-1)
Bsp1286I      825  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      221  TGTACA               T^GTACA
BspHI         449  TCATGA               T^CATGA
BspLU11I      224  ACATGT               A^CATGT
BspMI          18  GCAGGT               ACCTGC(4/8)
BsrDI         177  CATTGC               GCAATG(2/0)
BsrGI         221  TGTACA               T^GTACA
BstEII        885  GGTGACC              G^GT[ACGT]ACC
BstPI         885  GGTGACC              G^GT[ACGT]ACC
BstXI         281  CCAAGATGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        945  CCTGAGG              CC^T[ACGT]AGG
Cac8I         380  GCTTGC               GC[ACGT]^[ACGT]GC
Eco81I        945  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       885  GGTGACC              G^GT[ACGT]ACC
EcoT14I        87  CCAAGG               C^C[AT][AT]GG
FbaI          405  TGATCA               T^GATCA
Fnu4HI        940  GCAGC                GC^[ACGT]GC
HincII        292  GTCAAC               GT[CT]^[GA]AC
HindII        292  GTCAAC               GT[CT]^[GA]AC
HphI          885  GGTGA                GGTGA(8/7)
ItaI          940  GCAGC                GC^[ACGT]GC
NdeI          146  CATATG               CA^TATG
NspI          224  ACATGT               [GA]CATG^[CT]
PleI          833  GACTC                GAGTC(4/5)
RcaI          449  TCATGA               T^CATGA
SspBI         221  TGTACA               T^GTACA
StuI          752  AGGCCT               AGG^CCT
StyI           87  CCAAGG               C^C[AT][AT]GG
TfiI          626  GAATC                G^A[AT]TC
TseI          940  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BpmI          340  CTCCAG               CTGGAG(16/14)
              919  CTCCAG              
BsmFI           5  GTCCC                GGGAC(10/14)
              428  GTCCC               
BstF5I        331  GGATG                GGATG(2/0)
              851  GGATG               
BstYI         116  AGATCT               [GA]^GATC[CT]
              691  GGATCC              
Cfr13I        553  GGCCC                G^G[ACGT]CC
              589  GGGCC               
FokI          331  GGATG                GGATG(9/13)
              851  GGATG               
HinfI         626  GAATC                G^A[ACGT]TC
              833  GACTC               
MflI          116  AGATCT               [GA]^GATC[CT]
              691  GGATCC              
Sau96I        553  GGCCC                G^G[ACGT]CC
              589  GGGCC               
XhoII         116  AGATCT               [GA]^GATC[CT]
              691  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BsaJI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstNI       DdeI        DpnII       EarI        
EcoRII      HaeIII      Ksp632I     MaeIII      MboI        
MboII       MnlI        MslI        MvaI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      ScrFI       
SfaNI       SfcI        Tsp45I      Tsp509I     TspRI