Restriction map for
pFN21AE9683
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AgeI AhdI AlwNI AocI
ApaI AscI AspEI AspI AvaI
AviII AvrII BamHI BbeI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaJI BsgI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 855 GGTACC G^GTACC
AccIII 11 TCCGGA T^CCGGA
AflIII 312 ACGTGT A^C[GA][CT]GT
Alw44I 617 GTGCAC G^TGCAC
Aor51HI 81 AGCGCT AGC^GCT
ApaLI 617 GTGCAC G^TGCAC
AseI 172 ATTAAT AT^TAAT
AsnI 172 ATTAAT AT^TAAT
Asp718I 855 GGTACC G^GTACC
AspHI 617 GTGCAC G[AT]GC[AT]^C
AvaII 3 GGACC G^G[AT]CC
BalI 41 TGGCCA TGG^CCA
BanI 855 GGTACC G^G[CT][GA]CC
BanII 553 GAGCCC G[GA]GC[CT]^C
BbrPI 311 CACGTG CAC^GTG
BbsI 390 GAAGAC GAAGAC(2/6)
BcnI 68 CCCGG CC^[GC]GG
BpuAI 390 GAAGAC GAAGAC(2/6)
BsaI 35 GGTCTC GGTCTC(1/5)
BsaWI 11 TCCGGA [AT]^CCGG[AT]
BseAI 11 TCCGGA T^CCGGA
BseRI 745 GAGGAG GAGGAG(10/8)
BsiHKAI 617 GTGCAC G[AT]GC[AT]^C
BsmBI 61 GAGACG CGTCTC(1/5)
BsmFI 714 GGGAC GGGAC(10/14)
BspEI 11 TCCGGA T^CCGGA
BsrBI 490 GAGCGG CCGCTC(-3/-3)
BsrI 1178 CCAGT ACTGG(1/-1)
BssSI 334 CACGAG CACGAG(-5/-1)
BstYI 1026 AGATCC [GA]^GATC[CT]
CfoI 82 GCGC GCG^C
EaeI 41 TGGCCA [CT]^GGCC[GA]
Eco47III 81 AGCGCT AGC^GCT
FauI 23 GCGGG CCCGC(4/6)
HaeII 81 AGCGCT [GA]GCGC^[CT]
HhaI 82 GCGC GCG^C
HinP1I 82 GCGC G^CGC
HphI 1036 TCACC GGTGA(8/7)
KpnI 855 GGTACC GGTAC^C
MflI 1026 AGATCC [GA]^GATC[CT]
MluNI 41 TGGCCA TGG^CCA
MroI 11 TCCGGA T^CCGGA
MscI 41 TGGCCA TGG^CCA
NciI 68 CCCGG CC^[GC]GG
NspI 1006 ACATGC [GA]CATG^[CT]
PflMI 810 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 156 GACTC GAGTC(4/5)
PmaCI 311 CACGTG CAC^GTG
PmlI 311 CACGTG CAC^GTG
PshBI 172 ATTAAT AT^TAAT
PstI 73 CTGCAG CTGCA^G
SapI 550 GAAGAGC GCTCTTC(1/4)
SfaNI 829 GATGC GCATC(5/9)
SfcI 73 CTGCAG C^T[GA][CT]AG
SmlI 487 CTTGAG C^T[CT][GA]AG
SpeI 588 ACTAGT A^CTAGT
SspI 175 AATATT AAT^ATT
Van91I 810 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 842 CCAACTCTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1026 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 418 GATCC GGATC(4/5)
1027 GATCC
Asp700I 379 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1105 GAAGATATTC
BciVI 502 GTATCC GTATCC(6/5)
916 GTATCC
BfaI 589 CTAG C^TAG
1144 CTAG
BmyI 553 GAGCCC G[AGT]GC[ACT]^C
617 GTGCAC
BpmI 1082 CTCCAG CTGGAG(16/14)
1114 CTCCAG
BsaAI 311 CACGTG [CT]AC^GT[GA]
458 CACGTA
Bsp1286I 553 GAGCCC G[AGT]GC[ACT]^C
617 GTGCAC
BstNI 767 CCAGG CC^[AT]GG
874 CCTGG
BstXI 44 CCAGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
291 CCAGAAGTATGG
Cfr13I 3 GGACC G^G[ACGT]CC
66 GGCCC
DdeI 446 CTGAG C^T[ACGT]AG
1066 CTCAG
DraI 693 TTTAAA TTT^AAA
993 TTTAAA
EarI 1032 CTCTTC CTCTTC(1/4)
550 GAAGAG
EcoRII 767 CCAGG ^CC[AT]GG
874 CCTGG
HapII 12 CCGG C^CGG
69 CCGG
HpaII 12 CCGG C^CGG
69 CCGG
Ksp632I 1032 CTCTTC CTCTTC(1/4)
550 GAAGAG
MaeI 589 CTAG C^TAG
1144 CTAG
MaeII 312 ACGT A^CGT
459 ACGT
MspI 12 CCGG C^CGG
69 CCGG
MvaI 767 CCAGG CC^[AT]GG
874 CCTGG
MwoI 75 GCAGCGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1159 GCAATTAAAGC
NlaIII 1007 CATG CATG^
1198 CATG
Sau96I 3 GGACC G^G[ACGT]CC
66 GGCCC
ScaI 240 AGTACT AGT^ACT
597 AGTACT
TfiI 763 GAATC G^A[AT]TC
1059 GAATC
TspRI 533 CAGTG CA[GC]TG[ACGT][ACGT]^
1169 CAGTG
XmnI 379 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1105 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsiYI BslI BsmAI BssKI
BstF5I Cac8I DpnII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI MseI NdeII NlaIV
RsaI Sau3AI ScrFI Tru9I TseI
Tsp509I