################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccII AclI AcyI AflII AgeI AhdI AlwNI AocI ApaI AscI AspEI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaJI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 855 GGTACC G^GTACC AccIII 11 TCCGGA T^CCGGA AflIII 312 ACGTGT A^C[GA][CT]GT Alw44I 617 GTGCAC G^TGCAC Aor51HI 81 AGCGCT AGC^GCT ApaLI 617 GTGCAC G^TGCAC AseI 172 ATTAAT AT^TAAT AsnI 172 ATTAAT AT^TAAT Asp718I 855 GGTACC G^GTACC AspHI 617 GTGCAC G[AT]GC[AT]^C AvaII 3 GGACC G^G[AT]CC BalI 41 TGGCCA TGG^CCA BanI 855 GGTACC G^G[CT][GA]CC BanII 553 GAGCCC G[GA]GC[CT]^C BbrPI 311 CACGTG CAC^GTG BbsI 390 GAAGAC GAAGAC(2/6) BcnI 68 CCCGG CC^[GC]GG BpuAI 390 GAAGAC GAAGAC(2/6) BsaI 35 GGTCTC GGTCTC(1/5) BsaWI 11 TCCGGA [AT]^CCGG[AT] BseAI 11 TCCGGA T^CCGGA BseRI 745 GAGGAG GAGGAG(10/8) BsiHKAI 617 GTGCAC G[AT]GC[AT]^C BsmBI 61 GAGACG CGTCTC(1/5) BsmFI 714 GGGAC GGGAC(10/14) BspEI 11 TCCGGA T^CCGGA BsrBI 490 GAGCGG CCGCTC(-3/-3) BsrI 1178 CCAGT ACTGG(1/-1) BssSI 334 CACGAG CACGAG(-5/-1) BstYI 1026 AGATCC [GA]^GATC[CT] CfoI 82 GCGC GCG^C EaeI 41 TGGCCA [CT]^GGCC[GA] Eco47III 81 AGCGCT AGC^GCT FauI 23 GCGGG CCCGC(4/6) HaeII 81 AGCGCT [GA]GCGC^[CT] HhaI 82 GCGC GCG^C HinP1I 82 GCGC G^CGC HphI 1036 TCACC GGTGA(8/7) KpnI 855 GGTACC GGTAC^C MflI 1026 AGATCC [GA]^GATC[CT] MluNI 41 TGGCCA TGG^CCA MroI 11 TCCGGA T^CCGGA MscI 41 TGGCCA TGG^CCA NciI 68 CCCGG CC^[GC]GG NspI 1006 ACATGC [GA]CATG^[CT] PflMI 810 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 156 GACTC GAGTC(4/5) PmaCI 311 CACGTG CAC^GTG PmlI 311 CACGTG CAC^GTG PshBI 172 ATTAAT AT^TAAT PstI 73 CTGCAG CTGCA^G SapI 550 GAAGAGC GCTCTTC(1/4) SfaNI 829 GATGC GCATC(5/9) SfcI 73 CTGCAG C^T[GA][CT]AG SmlI 487 CTTGAG C^T[CT][GA]AG SpeI 588 ACTAGT A^CTAGT SspI 175 AATATT AAT^ATT Van91I 810 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 842 CCAACTCTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1026 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 418 GATCC GGATC(4/5) 1027 GATCC Asp700I 379 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1105 GAAGATATTC BciVI 502 GTATCC GTATCC(6/5) 916 GTATCC BfaI 589 CTAG C^TAG 1144 CTAG BmyI 553 GAGCCC G[AGT]GC[ACT]^C 617 GTGCAC BpmI 1082 CTCCAG CTGGAG(16/14) 1114 CTCCAG BsaAI 311 CACGTG [CT]AC^GT[GA] 458 CACGTA Bsp1286I 553 GAGCCC G[AGT]GC[ACT]^C 617 GTGCAC BstNI 767 CCAGG CC^[AT]GG 874 CCTGG BstXI 44 CCAGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 291 CCAGAAGTATGG Cfr13I 3 GGACC G^G[ACGT]CC 66 GGCCC DdeI 446 CTGAG C^T[ACGT]AG 1066 CTCAG DraI 693 TTTAAA TTT^AAA 993 TTTAAA EarI 1032 CTCTTC CTCTTC(1/4) 550 GAAGAG EcoRII 767 CCAGG ^CC[AT]GG 874 CCTGG HapII 12 CCGG C^CGG 69 CCGG HpaII 12 CCGG C^CGG 69 CCGG Ksp632I 1032 CTCTTC CTCTTC(1/4) 550 GAAGAG MaeI 589 CTAG C^TAG 1144 CTAG MaeII 312 ACGT A^CGT 459 ACGT MspI 12 CCGG C^CGG 69 CCGG MvaI 767 CCAGG CC^[AT]GG 874 CCTGG MwoI 75 GCAGCGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1159 GCAATTAAAGC NlaIII 1007 CATG CATG^ 1198 CATG Sau96I 3 GGACC G^G[ACGT]CC 66 GGCCC ScaI 240 AGTACT AGT^ACT 597 AGTACT TfiI 763 GAATC G^A[AT]TC 1059 GAATC TspRI 533 CAGTG CA[GC]TG[ACGT][ACGT]^ 1169 CAGTG XmnI 379 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1105 GAAGATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BsiYI BslI BsmAI BssKI BstF5I Cac8I DpnII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIV RsaI Sau3AI ScrFI Tru9I TseI Tsp509I