Restriction map for
pFN21AE1650
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# NO CUT ENZYMES ( 189 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI KspI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TfiI Tru9I Tsp45I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 24 GACGTC GACGT^C
AciI 235 CCGC CCGC(-3/-1)
AfaI 112 GTAC GT^AC
AflIII 74 ACACGT A^C[GA][CT]GT
AluI 290 AGCT AG^CT
AvaII 444 GGACC G^G[AT]CC
BanII 473 GAGCCC G[GA]GC[CT]^C
BcnI 264 CCGGG CC^[GC]GG
BfaI 292 CTAG C^TAG
BpmI 470 CTGGAG CTGGAG(16/14)
BsaJI 51 CCTGGG C^C[ACGT][ACGT]GG
BsmAI 215 GTCTC GTCTC(1/5)
Bsp1407I 111 TGTACA T^GTACA
BsrGI 111 TGTACA T^GTACA
BssSI 187 CTCGTG CACGAG(-5/-1)
BstNI 51 CCTGG CC^[AT]GG
DpnII 318 GATC ^GATC
DraII 15 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 172 GAAGAG CTCTTC(1/4)
EcoO109I 15 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 51 CCTGG ^CC[AT]GG
HapII 264 CCGG C^CGG
HgaI 226 GACGC GACGC(5/10)
HpaII 264 CCGG C^CGG
Ksp632I 172 GAAGAG CTCTTC(1/4)
MaeI 292 CTAG C^TAG
MboI 318 GATC ^GATC
MslI 369 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 264 CCGG C^CGG
MvaI 51 CCTGG CC^[AT]GG
NciI 264 CCGGG CC^[GC]GG
NdeII 318 GATC ^GATC
RsaI 112 GTAC GT^AC
Sau3AI 318 GATC ^GATC
SmlI 274 CTCAAG C^T[CT][GA]AG
SspBI 111 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 24 GACGTC G[GA]^CG[CT]C
226 GACGCC
BbvI 47 GCTGC GCAGC(8/12)
207 GCTGC
BcgI 23 CGACGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
440 GCATGGACCTCG
BsaHI 24 GACGTC G[GA]^CG[CT]C
226 GACGCC
BsmFI 138 GTCCC GGGAC(10/14)
192 GTCCC
BssKI 51 CCTGG ^CC[ACGT]GG
264 CCGGG
Cfr13I 16 GGCCC G^G[ACGT]CC
444 GGACC
DdeI 12 CTCAG C^T[ACGT]AG
217 CTCAG
Hin1I 24 GACGTC G[GA]^CG[CT]C
226 GACGCC
MaeII 25 ACGT A^CGT
76 ACGT
MboII 147 GAAGA GAAGA(8/7)
172 GAAGA
NlaIII 391 CATG CATG^
441 CATG
NlaIV 16 GGCCCC GG[ACGT]^[ACGT]CC
472 GGAGCC
Sau96I 16 GGCCC G^G[ACGT]CC
444 GGACC
ScrFI 51 CCTGG CC^[ACGT]GG
264 CCGGG
SfaNI 150 GATGC GCATC(5/9)
352 GATGC
TseI 47 GCTGC G^C[AT]GC
207 GCTGC
Tsp509I 131 AATT ^AATT
199 AATT
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AspHI BglI BmyI BsiHKAI Bsp1286I
BstF5I Cac8I Fnu4HI FokI HaeIII
ItaI MnlI MwoI TaqI TthHB8I