################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII AclI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 236 GGTACC G^GTACC AccII 1302 CGCG CG^CG AccIII 203 TCCGGA T^CCGGA AcsI 1723 GAATTT [GA]^AATT[CT] Alw44I 449 GTGCAC G^TGCAC ApaI 920 GGGCCC GGGCC^C ApaLI 449 GTGCAC G^TGCAC ApoI 1723 GAATTT [GA]^AATT[CT] Asp718I 236 GGTACC G^GTACC AspI 66 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1594 GGCGCC GGCGC^C BfaI 80 CTAG C^TAG BglII 1529 AGATCT A^GATCT BsaWI 203 TCCGGA [AT]^CCGG[AT] BseAI 203 TCCGGA T^CCGGA BsgI 738 CTGCAC GTGCAG(16/14) BspEI 203 TCCGGA T^CCGGA BsrDI 1637 CATTGC GCAATG(2/0) BssSI 1498 CTCGTG CACGAG(-5/-1) BstAPI 257 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1302 CGCG CG^CG DrdI 1116 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 602 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 489 CCCGC CCCGC(4/6) HgaI 422 GCGTC GACGC(5/10) KasI 1594 GGCGCC G^GCGCC KpnI 236 GGTACC GGTAC^C MaeI 80 CTAG C^TAG MroI 203 TCCGGA T^CCGGA MvnI 1302 CGCG CG^CG NarI 1594 GGCGCC GG^CGCC NotI 1303 GCGGCCGC GC^GGCCGC PflFI 66 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 408 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1120 CTGCAG CTGCA^G SacI 98 GAGCTC GAGCT^C SapI 657 GCTCTTC GCTCTTC(1/4) SfoI 1594 GGCGCC GGC^GCC SmlI 979 CTCAAG C^T[CT][GA]AG Tsp509I 1724 AATT ^AATT Tth111I 66 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 408 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 90 GTCTAC GT^[AC][GT]AC 151 GTAGAC AcyI 421 GGCGTC G[GA]^CG[CT]C 1594 GGCGCC AfaI 237 GTAC GT^AC 870 GTAC AlwNI 68 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 907 CAGATCCTG AspHI 98 GAGCTC G[AT]GC[AT]^C 449 GTGCAC BanI 236 GGTACC G^G[CT][GA]CC 1594 GGCGCC BbsI 1348 GTCTTC GAAGAC(2/6) 1615 GTCTTC BpuAI 1348 GTCTTC GAAGAC(2/6) 1615 GTCTTC BsaHI 421 GGCGTC G[GA]^CG[CT]C 1594 GGCGCC BsaI 895 GAGACC GGTCTC(1/5) 1626 GGTCTC BsiEI 874 CGGCCG CG[GA][CT]^CG 1304 CGGCCG BsiHKAI 98 GAGCTC G[AT]GC[AT]^C 449 GTGCAC DraIII 32 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 409 CACAACGTG EagI 874 CGGCCG C^GGCCG 1304 CGGCCG EarI 658 CTCTTC CTCTTC(1/4) 1684 CTCTTC EclXI 874 CGGCCG C^GGCCG 1304 CGGCCG Eco52I 874 CGGCCG C^GGCCG 1304 CGGCCG EcoT14I 510 CCTTGG C^C[AT][AT]GG 1199 CCTTGG Hin1I 421 GGCGTC G[GA]^CG[CT]C 1594 GGCGCC Ksp632I 658 CTCTTC CTCTTC(1/4) 1684 CTCTTC MaeII 308 ACGT A^CGT 413 ACGT MspA1I 189 CCGCTG C[AC]G^C[GT]G 877 CCGCTG NaeI 4 GCCGGC GCC^GGC 499 GCCGGC NgoMIV 4 GCCGGC G^CCGGC 499 GCCGGC PpuMI 1129 GGGACCT [GA]G^G[AT]CC[CT] 1583 GGGTCCT RsaI 237 GTAC GT^AC 870 GTAC SfcI 92 CTACAG C^T[GA][CT]AG 1120 CTGCAG StyI 510 CCTTGG C^C[AT][AT]GG 1199 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoO65I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp45I TspRI XhoII