Restriction map for
pFN21AE6486
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I AspEI AvaI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaBI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MluNI MscI
MseI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 236 GGTACC G^GTACC
AccII 1302 CGCG CG^CG
AccIII 203 TCCGGA T^CCGGA
AcsI 1723 GAATTT [GA]^AATT[CT]
Alw44I 449 GTGCAC G^TGCAC
ApaI 920 GGGCCC GGGCC^C
ApaLI 449 GTGCAC G^TGCAC
ApoI 1723 GAATTT [GA]^AATT[CT]
Asp718I 236 GGTACC G^GTACC
AspI 66 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1594 GGCGCC GGCGC^C
BfaI 80 CTAG C^TAG
BglII 1529 AGATCT A^GATCT
BsaWI 203 TCCGGA [AT]^CCGG[AT]
BseAI 203 TCCGGA T^CCGGA
BsgI 738 CTGCAC GTGCAG(16/14)
BspEI 203 TCCGGA T^CCGGA
BsrDI 1637 CATTGC GCAATG(2/0)
BssSI 1498 CTCGTG CACGAG(-5/-1)
BstAPI 257 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1302 CGCG CG^CG
DrdI 1116 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 602 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 489 CCCGC CCCGC(4/6)
HgaI 422 GCGTC GACGC(5/10)
KasI 1594 GGCGCC G^GCGCC
KpnI 236 GGTACC GGTAC^C
MaeI 80 CTAG C^TAG
MroI 203 TCCGGA T^CCGGA
MvnI 1302 CGCG CG^CG
NarI 1594 GGCGCC GG^CGCC
NotI 1303 GCGGCCGC GC^GGCCGC
PflFI 66 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 408 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1120 CTGCAG CTGCA^G
SacI 98 GAGCTC GAGCT^C
SapI 657 GCTCTTC GCTCTTC(1/4)
SfoI 1594 GGCGCC GGC^GCC
SmlI 979 CTCAAG C^T[CT][GA]AG
Tsp509I 1724 AATT ^AATT
Tth111I 66 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 408 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 90 GTCTAC GT^[AC][GT]AC
151 GTAGAC
AcyI 421 GGCGTC G[GA]^CG[CT]C
1594 GGCGCC
AfaI 237 GTAC GT^AC
870 GTAC
AlwNI 68 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
907 CAGATCCTG
AspHI 98 GAGCTC G[AT]GC[AT]^C
449 GTGCAC
BanI 236 GGTACC G^G[CT][GA]CC
1594 GGCGCC
BbsI 1348 GTCTTC GAAGAC(2/6)
1615 GTCTTC
BpuAI 1348 GTCTTC GAAGAC(2/6)
1615 GTCTTC
BsaHI 421 GGCGTC G[GA]^CG[CT]C
1594 GGCGCC
BsaI 895 GAGACC GGTCTC(1/5)
1626 GGTCTC
BsiEI 874 CGGCCG CG[GA][CT]^CG
1304 CGGCCG
BsiHKAI 98 GAGCTC G[AT]GC[AT]^C
449 GTGCAC
DraIII 32 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
409 CACAACGTG
EagI 874 CGGCCG C^GGCCG
1304 CGGCCG
EarI 658 CTCTTC CTCTTC(1/4)
1684 CTCTTC
EclXI 874 CGGCCG C^GGCCG
1304 CGGCCG
Eco52I 874 CGGCCG C^GGCCG
1304 CGGCCG
EcoT14I 510 CCTTGG C^C[AT][AT]GG
1199 CCTTGG
Hin1I 421 GGCGTC G[GA]^CG[CT]C
1594 GGCGCC
Ksp632I 658 CTCTTC CTCTTC(1/4)
1684 CTCTTC
MaeII 308 ACGT A^CGT
413 ACGT
MspA1I 189 CCGCTG C[AC]G^C[GT]G
877 CCGCTG
NaeI 4 GCCGGC GCC^GGC
499 GCCGGC
NgoMIV 4 GCCGGC G^CCGGC
499 GCCGGC
PpuMI 1129 GGGACCT [GA]G^G[AT]CC[CT]
1583 GGGTCCT
RsaI 237 GTAC GT^AC
870 GTAC
SfcI 92 CTACAG C^T[GA][CT]AG
1120 CTGCAG
StyI 510 CCTTGG C^C[AT][AT]GG
1199 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoO65I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp45I TspRI XhoII